FastQCFastQC Report
Fri 17 Jun 2016
SRR1527966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036267
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAGGACTGGGGACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCC37520.18425874406450626No Hit
GAACAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC37010.1817541609229045No Hit
ACAAAGGACTGGGGGACACAGGATCTGGAGCTGTGCTGATAACAGCGGAATC36360.17856204515419638No Hit
GGGCAGGACTGGGGAAGGAAAGAAAGAAAGAAAGAAAGGAAGTGAAAGAAAG32720.16068619684943083No Hit
GCTCAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC26720.13122051283058656No Hit
CCTGAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC22820.11206781821833778No Hit
TAATAGGACTGGGGATGTGGATTGTCTCAAAGCCTGCCAGGAGCAAATCGAA22730.11162583295805512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA750.046.00000445
ACAGTCG351.02008926E-746.00000430
CGAACAA351.02008926E-746.00000429
CGTATAG700.046.00000425
CCGATCA351.02008926E-746.00000437
CTATATG351.02008926E-746.00000415
ATAATCG700.046.00000442
CACGATT351.02008926E-746.00000424
TAAGTCG351.02008926E-746.00000428
TAAGTCC351.02008926E-746.00000422
ATGTAAT700.046.00000425
ACGATTG351.02008926E-746.00000425
ACGATAT351.02008926E-746.00000445
ATCCCGT351.02008926E-746.00000440
CGAATAA750.046.00000415
TCGTACC351.02008926E-746.00000445
CGTAATC750.046.00000428
TATAGAC351.02008926E-746.00000422
CAATGCG351.02008926E-746.00000442
CGCGTAT700.046.00000423