##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527966_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2036267
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79878522806685	31.0	31.0	34.0	30.0	34.0
2	32.363860927864565	34.0	31.0	34.0	30.0	34.0
3	32.50572591904696	34.0	31.0	34.0	31.0	34.0
4	36.05341981184196	37.0	35.0	37.0	35.0	37.0
5	36.02978391340625	37.0	35.0	37.0	35.0	37.0
6	35.98920131790183	37.0	35.0	37.0	35.0	37.0
7	35.72369242343956	37.0	35.0	37.0	33.0	37.0
8	35.74383614722431	37.0	35.0	37.0	33.0	37.0
9	37.72471193610661	39.0	38.0	39.0	35.0	39.0
10	37.94621432258147	39.0	38.0	39.0	35.0	39.0
11	38.1600669263903	39.0	39.0	39.0	37.0	39.0
12	38.17155215892611	39.0	39.0	39.0	37.0	39.0
13	38.15992794658068	39.0	39.0	39.0	37.0	39.0
14	38.810326445402296	40.0	39.0	40.0	36.0	40.0
15	38.166151590140196	40.0	38.0	40.0	34.0	40.0
16	37.58591972467265	40.0	37.0	40.0	33.0	40.0
17	37.56427767085554	40.0	37.0	40.0	33.0	40.0
18	37.595961629786274	40.0	37.0	40.0	33.0	40.0
19	37.760814274355965	40.0	37.0	40.0	33.0	40.0
20	37.78734664953073	40.0	37.0	40.0	33.0	40.0
21	37.635671058854264	40.0	37.0	40.0	33.0	40.0
22	37.69635268852267	40.0	37.0	40.0	33.0	40.0
23	37.59678126689673	40.0	37.0	40.0	33.0	40.0
24	37.59557366494669	40.0	37.0	40.0	33.0	40.0
25	37.704158148219264	40.0	37.0	40.0	33.0	40.0
26	37.67949880835863	40.0	37.0	40.0	33.0	40.0
27	37.624282080886246	40.0	37.0	40.0	33.0	40.0
28	37.595007923813526	40.0	37.0	40.0	33.0	40.0
29	37.58910692949402	40.0	37.0	40.0	33.0	40.0
30	37.56375367277474	40.0	37.0	40.0	33.0	40.0
31	37.50383962417502	40.0	37.0	40.0	33.0	40.0
32	37.42277265211291	40.0	36.0	40.0	33.0	40.0
33	37.35787939400874	40.0	36.0	40.0	33.0	40.0
34	37.251109505776995	40.0	36.0	40.0	33.0	40.0
35	37.1514600000884	40.0	35.0	40.0	33.0	40.0
36	37.0496693213611	39.0	35.0	40.0	32.0	40.0
37	36.91790762213403	39.0	35.0	40.0	32.0	40.0
38	36.72694297948157	39.0	35.0	40.0	32.0	40.0
39	36.572642978548494	39.0	35.0	40.0	31.0	40.0
40	36.44738632016332	39.0	35.0	40.0	31.0	40.0
41	36.22494348727353	38.0	35.0	40.0	31.0	40.0
42	36.06827591862953	38.0	35.0	40.0	30.0	40.0
43	35.868803550811364	38.0	35.0	40.0	30.0	40.0
44	35.69210815673976	38.0	35.0	40.0	30.0	40.0
45	35.51595836891724	37.0	35.0	40.0	30.0	40.0
46	35.34765971260154	37.0	35.0	40.0	29.0	40.0
47	35.140244378561356	37.0	34.0	40.0	29.0	40.0
48	34.9640867332231	36.0	34.0	40.0	28.0	40.0
49	34.797949875924914	36.0	34.0	40.0	28.0	40.0
50	34.62617574217919	35.0	34.0	39.0	27.0	40.0
51	34.43850634518951	35.0	34.0	39.0	27.0	40.0
52	33.809084466820906	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	5.0
15	10.0
16	35.0
17	118.0
18	289.0
19	612.0
20	1088.0
21	2057.0
22	2995.0
23	4377.0
24	6381.0
25	9152.0
26	12820.0
27	17295.0
28	22722.0
29	29946.0
30	37643.0
31	46190.0
32	56020.0
33	70643.0
34	101666.0
35	173386.0
36	186837.0
37	357962.0
38	604521.0
39	291491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.0480565662558	22.433747637220463	34.04833452587505	11.46986127064869
2	26.94548406471253	21.190393990571962	34.44621947907617	17.41790246563933
3	19.076771366426897	22.33449739155032	36.460002543870715	22.12872869815206
4	20.364814633837312	24.680064058397058	32.884980211337705	22.070141096427925
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.50021288956704	0.37382131125240453	0.09104896361822885	0.03491683556233048
12	99.37252825881872	0.41281423310400844	0.11481794872676324	0.09983955935051739
13	98.00748133717238	1.0344419469548936	0.42391297408443984	0.5341637417882822
14	64.51870015081519	22.21324610181278	5.075365853299199	8.192687894072831
15	33.832547499910376	26.579176502884938	17.25981907087823	22.32845692632646
16	27.843008799926533	18.72426356661479	28.10353455612648	25.329193077332196
17	22.80108649798872	17.26748014872313	30.011535815293378	29.91989753799477
18	24.115010457862354	19.358954400380696	31.108346793421493	25.41768834833546
19	25.579946048332562	18.571729542343906	29.05046342154541	26.797860987778126
20	28.429768787688452	18.854600108924814	25.252926065196757	27.462705038189984
21	30.692782429809057	18.636112061925083	20.30097231846315	30.37013318980271
22	30.247113959024034	20.126584578544954	21.78884203299469	27.83745942943632
23	30.055488793954822	19.488947176377163	21.338360833819927	29.11720319584809
24	29.75818004220468	20.963901099413782	20.911206634493414	28.366712223888126
25	32.14450757194415	19.254940535794177	22.09715130677853	26.50340058548314
26	32.258736206990534	18.466340612503174	20.808469616214374	28.46645356429191
27	29.904771820198434	21.931848819432815	20.6736150023548	27.48976435801395
28	31.25926020507134	20.008328966682658	20.02812008444865	28.70429074379735
29	30.015661011056018	20.45222949642655	20.926970775443497	28.60513871707394
30	31.042147223325824	19.271343099898	20.638845495212564	29.047664181563615
31	31.07082715577083	19.074315892758662	20.390155122093518	29.464701829376992
32	32.01937663381079	20.806259689912963	19.317997099594503	27.85636657668174
33	30.780786606078674	21.805784801305524	19.815377845832593	27.59805074678321
34	30.120313298796276	21.997213528481286	20.737359098782232	27.145114073940206
35	31.779918841684317	22.91448027198791	19.21108577607946	26.094515110248313
36	32.41495344176378	23.32022274092739	19.13481876394402	25.13000505336481
37	31.785369993227803	22.33435006313023	19.040577684557082	26.839702259084884
38	30.89201956324981	23.723607955145372	20.998326840242463	24.386045641362355
39	30.91426615468404	23.86293153108114	22.246296777387247	22.976505536847576
40	31.30311496478605	23.680440728057764	20.17333679718819	24.843107509967997
41	31.37618986115279	24.655705759608146	19.163351367968936	24.804753011270133
42	33.613028153969985	22.85338808712217	17.716635392117045	25.816948366790797
43	32.114059698458014	23.868873777358274	18.553313489832128	25.463753034351583
44	31.665837535058028	24.949625957696117	18.59643160744637	24.78810489979949
45	29.44996898736757	22.508443146208233	20.52343823280542	27.518149633618773
46	30.249323885325452	20.63580070786395	21.195697813695354	27.919177593115247
47	30.143689408117897	20.953293453166996	22.484428613732874	26.418588524982233
48	28.91993044134193	21.02302890534493	23.379792532118824	26.677248121194324
49	29.9854586849367	20.63427831418964	22.0112588378636	27.369004163010057
50	30.25452948950211	21.903905529088277	20.948628053197346	26.892936928212265
51	28.997474299784855	22.029822218795474	21.614012307816214	27.35869117360346
52	29.419766661248254	22.11497804560993	21.602815347889052	26.86243994525276
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	1.0
8	0.0
9	8.5
10	17.0
11	80.5
12	144.0
13	247.0
14	473.5
15	597.0
16	872.5
17	1148.0
18	1304.5
19	1461.0
20	1737.5
21	2014.0
22	2438.0
23	2862.0
24	4065.0
25	5268.0
26	7503.5
27	9739.0
28	10951.0
29	12163.0
30	12855.5
31	13548.0
32	13784.5
33	14021.0
34	14724.0
35	15427.0
36	16219.0
37	17011.0
38	18900.0
39	21495.5
40	22202.0
41	25678.0
42	29154.0
43	37413.0
44	45672.0
45	45655.0
46	45638.0
47	55610.0
48	65582.0
49	71119.0
50	76656.0
51	89047.5
52	101439.0
53	112597.5
54	123756.0
55	134317.5
56	144879.0
57	162893.0
58	180907.0
59	188703.5
60	196500.0
61	197445.0
62	198390.0
63	187501.0
64	157025.5
65	137439.0
66	126173.0
67	114907.0
68	101374.0
69	87841.0
70	79133.0
71	70425.0
72	59799.5
73	49174.0
74	39244.0
75	29314.0
76	21720.0
77	14126.0
78	9676.0
79	5226.0
80	3960.0
81	2694.0
82	1901.0
83	1108.0
84	570.0
85	32.0
86	24.0
87	16.0
88	15.0
89	7.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2036267.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.773796490520956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.73245237876768	6.644611248993772
2	7.797885614895033	1.3683412288289976
3	3.6412606765033892	0.9584304043373219
4	2.245436636239885	0.7880401631491474
5	1.500735239797785	0.6583572790069465
6	0.9631489989144781	0.5070283983914773
7	0.6374357482875012	0.3914912071880234
8	0.5065899760271566	0.3555773883040129
9	0.3749245059827016	0.29605601833211975
>10	2.6836431335424753	4.705843137019669
>50	0.697541507511137	4.573059687829726
>100	2.93328036195717	60.81399183205677
>500	0.26481785826351706	15.031191301776428
>1k	0.020847363310106663	2.9079807047855892
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCAAGGACTGGGGACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCC	3752	0.18425874406450626	No Hit
GAACAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC	3701	0.1817541609229045	No Hit
ACAAAGGACTGGGGGACACAGGATCTGGAGCTGTGCTGATAACAGCGGAATC	3636	0.17856204515419638	No Hit
GGGCAGGACTGGGGAAGGAAAGAAAGAAAGAAAGAAAGGAAGTGAAAGAAAG	3272	0.16068619684943083	No Hit
GCTCAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC	2672	0.13122051283058656	No Hit
CCTGAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC	2282	0.11206781821833778	No Hit
TAATAGGACTGGGGATGTGGATTGTCTCAAAGCCTGCCAGGAGCAAATCGAA	2273	0.11162583295805512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0027010210350607265	0.0	0.0	0.0	0.0
12	0.0071699831112521095	0.0	0.0	0.0	0.0
13	0.009821894672948096	0.0	0.0	0.0	0.0
14	0.04262702288059474	0.0	0.0	0.0	0.0
15	0.0721909258461685	0.0	0.0	0.0	0.0
16	0.08928102257709819	0.0	0.0	0.0	0.0
17	0.10273701827903708	0.0	0.0	0.0	0.0
18	0.12051464763707313	0.0	0.0	0.0	0.0
19	0.13750652542127334	0.0	0.0	0.0	0.0
20	0.1562663442466042	0.0	0.0	0.0	0.0
21	0.17478061570511136	0.0	0.0	0.0	0.0
22	0.19633967451223244	0.0	0.0	0.0	0.0
23	0.21627812069831706	0.0	0.0	0.0	0.0
24	0.23808272687226184	0.0	0.0	0.0	0.0
25	0.2621463688209847	0.0	0.0	0.0	0.0
26	0.2929871180940417	0.0	0.0	0.0	0.0
27	0.3223054736927918	0.0	0.0	0.0	0.0
28	0.35324444191257826	0.0	0.0	0.0	0.0
29	0.3871790880076139	0.0	0.0	0.0	0.0
30	0.4327526792900931	0.0	0.0	0.0	0.0
31	0.4754779211174173	0.0	0.0	0.0	0.0
32	0.5138324198152796	0.0	0.0	0.0	0.0
33	0.5419721480532759	0.0	0.0	0.0	0.0
34	0.5711431752319318	0.0	0.0	0.0	0.0
35	0.5951085982339251	0.0	0.0	0.0	0.0
36	0.6202526485966723	0.0	0.0	0.0	0.0
37	0.6435305389715592	0.0	0.0	0.0	0.0
38	0.669313012488048	0.0	0.0	0.0	0.0
39	0.6967652080989379	0.0	0.0	0.0	0.0
40	0.7279006142121833	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAA	75	0.0	46.000004	45
ACAGTCG	35	1.02008926E-7	46.000004	30
CGAACAA	35	1.02008926E-7	46.000004	29
CGTATAG	70	0.0	46.000004	25
CCGATCA	35	1.02008926E-7	46.000004	37
CTATATG	35	1.02008926E-7	46.000004	15
ATAATCG	70	0.0	46.000004	42
CACGATT	35	1.02008926E-7	46.000004	24
TAAGTCG	35	1.02008926E-7	46.000004	28
TAAGTCC	35	1.02008926E-7	46.000004	22
ATGTAAT	70	0.0	46.000004	25
ACGATTG	35	1.02008926E-7	46.000004	25
ACGATAT	35	1.02008926E-7	46.000004	45
ATCCCGT	35	1.02008926E-7	46.000004	40
CGAATAA	75	0.0	46.000004	15
TCGTACC	35	1.02008926E-7	46.000004	45
CGTAATC	75	0.0	46.000004	28
TATAGAC	35	1.02008926E-7	46.000004	22
CAATGCG	35	1.02008926E-7	46.000004	42
CGCGTAT	70	0.0	46.000004	23
>>END_MODULE