FastQCFastQC Report
Fri 17 Jun 2016
SRR1527965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020132
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAGGACTGGGGACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCC35610.17627560971263265No Hit
GAACAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC35020.17335500848459406No Hit
ACAAAGGACTGGGGGACACAGGATCTGGAGCTGTGCTGATAACAGCGGAATC34970.17310749990594673No Hit
GGGCAGGACTGGGGAAGGAAAGAAAGAAAGAAAGAAAGGAAGTGAAAGAAAG30010.14855464890413103No Hit
GCTCAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC25880.1281104403078611No Hit
TAATAGGACTGGGGATGTGGATTGTCTCAAAGCCTGCCAGGAGCAAATCGAA21790.10786423857450898No Hit
CCTGAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC21680.10731971970148485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACT351.02008926E-746.00000423
TTTAACG351.02008926E-746.00000416
CCAATTA351.02008926E-746.00000431
CGACCAA351.02008926E-746.00000433
TCGGATT351.02008926E-746.00000431
TACGCGG351.02008926E-746.00000435
CGCTCAT351.02008926E-746.00000443
TACGATT351.02008926E-746.00000431
TTAAGCG351.02008926E-746.00000429
GTATAAT351.02008926E-746.00000427
ATAACGG351.02008926E-746.00000432
TGTACGC351.02008926E-746.00000422
TCGACAA351.02008926E-746.00000417
CTAATGG351.02008926E-746.00000443
GTACGGG351.02008926E-746.00000442
GTTGCGT351.02008926E-746.00000446
CTTAAGT301.8621085E-646.034
ACAACGA301.8621085E-646.014
AACGTTG551.8189894E-1246.020
CTGCTAT253.4180823E-546.019