##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527965_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2020132
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74013579310659	31.0	31.0	34.0	30.0	34.0
2	32.202916443083915	34.0	31.0	34.0	30.0	34.0
3	32.43457160225174	34.0	31.0	34.0	30.0	34.0
4	36.01975316464468	37.0	35.0	37.0	35.0	37.0
5	36.011784378446556	37.0	35.0	37.0	35.0	37.0
6	35.9845381390919	37.0	35.0	37.0	35.0	37.0
7	35.72005146198367	37.0	35.0	37.0	33.0	37.0
8	35.701575936621964	37.0	35.0	37.0	33.0	37.0
9	37.71967277385834	39.0	38.0	39.0	35.0	39.0
10	37.9371174754917	39.0	38.0	39.0	35.0	39.0
11	38.14608401827207	39.0	39.0	39.0	37.0	39.0
12	38.15045353471951	39.0	39.0	39.0	37.0	39.0
13	38.16096819415761	39.0	39.0	39.0	37.0	39.0
14	38.83411331536751	40.0	39.0	40.0	36.0	40.0
15	38.187603087323005	40.0	38.0	40.0	34.0	40.0
16	37.61661267679538	40.0	37.0	40.0	33.0	40.0
17	37.60885130278616	40.0	37.0	40.0	33.0	40.0
18	37.66155577952332	40.0	37.0	40.0	33.0	40.0
19	37.81780200501749	40.0	37.0	40.0	33.0	40.0
20	37.822145780572754	40.0	37.0	40.0	33.0	40.0
21	37.85302396081048	40.0	37.0	40.0	34.0	40.0
22	37.80633641762024	40.0	37.0	40.0	33.0	40.0
23	37.69427393853471	40.0	37.0	40.0	33.0	40.0
24	37.69626440252419	40.0	37.0	40.0	33.0	40.0
25	37.6417531131629	40.0	37.0	40.0	33.0	40.0
26	37.599978120241644	40.0	37.0	40.0	33.0	40.0
27	37.581693176485494	40.0	37.0	40.0	33.0	40.0
28	37.4107399912481	40.0	37.0	40.0	33.0	40.0
29	37.508053929149185	40.0	37.0	40.0	33.0	40.0
30	37.54670041363634	40.0	37.0	40.0	33.0	40.0
31	37.526021071890355	40.0	37.0	40.0	33.0	40.0
32	37.443972968103076	40.0	36.0	40.0	33.0	40.0
33	37.378490118467504	40.0	36.0	40.0	33.0	40.0
34	37.292797698368226	40.0	36.0	40.0	33.0	40.0
35	37.12638679056616	40.0	35.0	40.0	33.0	40.0
36	36.65029364417771	39.0	35.0	40.0	31.0	40.0
37	36.733768882429466	39.0	35.0	40.0	31.0	40.0
38	36.66202258070265	39.0	35.0	40.0	31.0	40.0
39	36.543769417048	39.0	35.0	40.0	31.0	40.0
40	36.4284957616631	39.0	35.0	40.0	31.0	40.0
41	36.240319939489105	38.0	35.0	40.0	31.0	40.0
42	35.94138254331895	38.0	35.0	40.0	30.0	40.0
43	35.741601538909336	38.0	35.0	40.0	30.0	40.0
44	35.57502331530811	38.0	35.0	40.0	29.0	40.0
45	35.15603782327096	37.0	34.0	40.0	28.0	40.0
46	35.140383895705824	37.0	34.0	40.0	28.0	40.0
47	35.013518918565715	37.0	34.0	40.0	28.0	40.0
48	34.83504691772617	36.0	34.0	40.0	28.0	40.0
49	34.6896385978738	36.0	34.0	40.0	27.0	40.0
50	34.446331229840425	35.0	34.0	39.0	27.0	40.0
51	34.35298534947221	35.0	34.0	39.0	27.0	40.0
52	33.856836582955964	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	6.0
15	16.0
16	35.0
17	96.0
18	276.0
19	642.0
20	1149.0
21	1982.0
22	3060.0
23	4527.0
24	6548.0
25	9362.0
26	12940.0
27	17260.0
28	22926.0
29	30272.0
30	38217.0
31	46934.0
32	56866.0
33	72057.0
34	102729.0
35	173491.0
36	189014.0
37	359810.0
38	583770.0
39	286143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.051915419388436	22.488134438739646	33.953771337714564	11.506178804157353
2	27.12085150871329	21.230345343769617	34.2461284708128	17.402674676704294
3	19.071377513944636	22.370369857019245	36.44355913375958	22.114693495276548
4	20.36663940772187	24.657547130583545	32.84874453748567	22.127068924208913
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.4943399738235	0.37992566822366064	0.09083564836357229	0.034898709589274365
12	99.36429896660218	0.41784398247243254	0.11642803539570683	0.10142901552967826
13	98.00201174972725	1.039536030318811	0.4262097724307124	0.5322424475232312
14	64.50543825849005	22.266218247124446	5.077093972077072	8.151249522308444
15	33.875806135440655	26.565838271954505	17.225854548118637	22.332501044486204
16	27.9124334449432	18.770258577162284	28.015298010229035	25.30200996766548
17	22.859595313573568	17.274217724386325	29.92764829228981	29.938538669750294
18	24.089217932293533	19.375268546807835	31.14380644433136	25.391707076567272
19	25.72059647587385	18.564925460316452	28.98964028093214	26.724837782877554
20	28.474970942492867	18.798722063706727	25.313494365714718	27.412812628085693
21	30.435783404252792	18.68145249914362	20.308969908897044	30.573794187706543
22	30.289901847998053	20.167395001910766	21.78728914744185	27.75541400264933
23	30.000564319559313	19.511150756485222	21.373207295364857	29.11507762859061
24	29.720731120540638	21.00229093940396	20.933978571697295	28.342999368358107
25	32.15977965796294	19.289927588890233	22.043411024626113	26.50688172852071
26	32.197994982506096	18.52002740414983	20.84235089588205	28.43962671746203
27	29.866117659638082	22.000691043951583	20.627117435890327	27.506073860520008
28	31.338793702589733	20.028245678995233	20.094082960915426	28.53887765749961
29	30.007247051182794	20.431635160474663	20.920266596440232	28.64085119190231
30	31.127817390150742	19.282304324667894	20.60469315866488	28.985185126516484
31	31.08445388717173	19.064546277173967	20.39064773985066	29.46035209580364
32	32.004938291161174	20.949621113867806	19.215279001570195	27.830161593400828
33	30.81684761193823	21.828722083507415	19.82316007072805	27.531270233826305
34	30.127585722121133	22.061726659446016	20.705429150174346	27.10525846825851
35	31.806386909370275	22.920482423920813	19.228297952807043	26.044832713901865
36	32.49441125629414	23.226304023697462	19.191072662578485	25.08821205742991
37	31.750796482606088	22.38739844723018	19.062665211976245	26.799139858187488
38	30.83635128793564	23.71250987559229	21.075998994125136	24.375139842346936
39	30.810065876883293	23.8758160357838	22.308146200347302	23.005971886985602
40	31.367455195997096	23.74493349939509	20.144376704096565	24.743234600511254
41	31.37280138129588	24.730166147558673	19.11721610271012	24.77981636843533
42	33.74170598752953	22.8463783554738	17.728940485077214	25.68297517191946
43	32.15077034570018	23.798197345519995	18.572697229685982	25.47833507909384
44	31.802377270396192	24.921787289147442	18.499583195553555	24.776252244902807
45	29.483964414206596	22.53219096573887	20.60177255743684	27.38207206261769
46	30.38133151695038	20.662709169499816	21.157577821647298	27.798381491902507
47	30.194314034924453	20.91561343516166	22.453532739444746	26.436539790469137
48	28.95800868458101	21.114263820384014	23.297487490916435	26.630240004118544
49	30.03858163723955	20.680331780299504	22.023214324608492	27.257872257852455
50	30.34856138113747	21.865501858294408	21.008033138428576	26.777903622139544
51	28.911031556353745	22.067270851607717	21.595767009284543	27.425930582754
52	29.48530096053129	22.109198804830577	21.569085584506357	26.836414650131772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	11.5
10	20.0
11	83.0
12	146.0
13	242.5
14	477.5
15	616.0
16	845.5
17	1075.0
18	1228.0
19	1381.0
20	1662.5
21	1944.0
22	2379.0
23	2814.0
24	3964.5
25	5115.0
26	7327.0
27	9539.0
28	10755.5
29	11972.0
30	12762.0
31	13552.0
32	13812.5
33	14073.0
34	14609.5
35	15146.0
36	16011.0
37	16876.0
38	18712.0
39	21422.5
40	22297.0
41	25883.0
42	29469.0
43	37424.0
44	45379.0
45	45618.0
46	45857.0
47	55279.5
48	64702.0
49	70498.5
50	76295.0
51	88031.5
52	99768.0
53	111558.5
54	123349.0
55	133740.0
56	144131.0
57	162046.5
58	179962.0
59	187348.5
60	194735.0
61	195443.5
62	196152.0
63	185355.5
64	155596.0
65	136633.0
66	125778.0
67	114923.0
68	100551.5
69	86180.0
70	77964.5
71	69749.0
72	59237.5
73	48726.0
74	38873.0
75	29020.0
76	21493.0
77	13966.0
78	9569.5
79	5173.0
80	3948.0
81	2723.0
82	1937.5
83	1152.0
84	600.5
85	49.0
86	30.0
87	11.0
88	10.5
89	6.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2020132.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.871193999452274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53145961400706	7.554568849111106
2	8.054260772943476	1.5901034122380697
3	3.6486604853655713	1.0804990646753772
4	2.1594939907334116	0.8526713649272359
5	1.386313077634079	0.6842282666651868
6	0.8559481643118417	0.5069538230038344
7	0.6123326639099054	0.42311181623592586
8	0.4588161773137098	0.36232507970805783
9	0.3479532207168989	0.30912423699876707
>10	2.3819288437856194	4.703769632303066
>50	0.6725545152425226	4.990770729742246
>100	2.658683990693403	60.73133479381821
>500	0.21556897221838406	13.649070580742315
>1k	0.016025511124148455	2.5614683498306055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCAAGGACTGGGGACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCC	3561	0.17627560971263265	No Hit
GAACAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC	3502	0.17335500848459406	No Hit
ACAAAGGACTGGGGGACACAGGATCTGGAGCTGTGCTGATAACAGCGGAATC	3497	0.17310749990594673	No Hit
GGGCAGGACTGGGGAAGGAAAGAAAGAAAGAAAGAAAGGAAGTGAAAGAAAG	3001	0.14855464890413103	No Hit
GCTCAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC	2588	0.1281104403078611	No Hit
TAATAGGACTGGGGATGTGGATTGTCTCAAAGCCTGCCAGGAGCAAATCGAA	2179	0.10786423857450898	No Hit
CCTGAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCC	2168	0.10731971970148485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0027720960808501624	0.0	0.0	0.0	0.0
12	0.006534226476289668	0.0	0.0	0.0	0.0
13	0.009207319125680897	0.0	0.0	0.0	0.0
14	0.03856183655325494	0.0	0.0	0.0	0.0
15	0.070143931188655	0.0	0.0	0.0	0.0
16	0.08638049394792023	0.0	0.0	0.0	0.0
17	0.10133001209821932	0.0	0.0	0.0	0.0
18	0.12004166064395792	0.0	0.0	0.0	0.0
19	0.13474367021560968	0.0	0.0	0.0	0.0
20	0.1548908685175028	0.0	0.0	0.0	0.0
21	0.1744935479463718	0.0	0.0	0.0	0.0
22	0.19800686291786873	0.0	0.0	0.0	0.0
23	0.2187480818085155	0.0	0.0	0.0	0.0
24	0.24216239334855347	0.0	0.0	0.0	0.0
25	0.26483417915264945	0.0	0.0	0.0	0.0
26	0.2965647789352379	0.0	0.0	0.0	0.0
27	0.3283943821492853	0.0	0.0	0.0	0.0
28	0.3593329544802023	0.0	0.0	0.0	0.0
29	0.3930436228919694	0.0	0.0	0.0	0.0
30	0.4372486550383836	0.0	0.0	0.0	0.0
31	0.47808757051519407	0.0	0.0	0.0	0.0
32	0.5170949225100142	0.0	0.0	0.0	0.0
33	0.5455584090544578	0.0	0.0	0.0	0.0
34	0.5732793698629595	0.0	0.0	0.0	0.0
35	0.599713286062495	0.0	0.0	0.0	0.0
36	0.625157167947441	0.0	0.0	0.0	0.0
37	0.6503535412537399	0.0	0.0	0.0	0.0
38	0.6763419420117102	0.0	0.0	0.0	0.0
39	0.7040629028202118	0.0	0.0	0.0	0.0
40	0.7359420077499886	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACT	35	1.02008926E-7	46.000004	23
TTTAACG	35	1.02008926E-7	46.000004	16
CCAATTA	35	1.02008926E-7	46.000004	31
CGACCAA	35	1.02008926E-7	46.000004	33
TCGGATT	35	1.02008926E-7	46.000004	31
TACGCGG	35	1.02008926E-7	46.000004	35
CGCTCAT	35	1.02008926E-7	46.000004	43
TACGATT	35	1.02008926E-7	46.000004	31
TTAAGCG	35	1.02008926E-7	46.000004	29
GTATAAT	35	1.02008926E-7	46.000004	27
ATAACGG	35	1.02008926E-7	46.000004	32
TGTACGC	35	1.02008926E-7	46.000004	22
TCGACAA	35	1.02008926E-7	46.000004	17
CTAATGG	35	1.02008926E-7	46.000004	43
GTACGGG	35	1.02008926E-7	46.000004	42
GTTGCGT	35	1.02008926E-7	46.000004	46
CTTAAGT	30	1.8621085E-6	46.0	34
ACAACGA	30	1.8621085E-6	46.0	14
AACGTTG	55	1.8189894E-12	46.0	20
CTGCTAT	25	3.4180823E-5	46.0	19
>>END_MODULE