FastQCFastQC Report
Fri 17 Jun 2016
SRR1527964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033804
Sequences flagged as poor quality0
Sequence length59
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT38750.19052966755891915No Hit
GCCAAGGACTGGGGACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCTA37760.1856619418587042No Hit
ACAAAGGACTGGGGGACACAGGATCTGGAGCTGTGCTGATAACAGCGGAATCCCCTGTC37460.18418687346469964No Hit
GGGCAGGACTGGGGAAGGAAAGAAAGAAAGAAAGAAAGGAAGTGAAAGAAAGAAAGAAA35370.1739105636531347No Hit
GCTCAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT26740.13147776285227092No Hit
CCTGAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT22560.11092514322914107No Hit
TTATAGGACTGGGGGACAACCAATGACCTTGACACTGCCTCTTAGGCACTTAGCTCTAG20870.10261559127624884No Hit
TAATAGGACTGGGGATGTGGATTGTCTCAAAGCCTGCCAGGAGCAAATCGAAGCTCTGC20750.10202556391864703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG503.6379788E-1253.020
AGGTATA150.006697003753.030
TGATACG203.13882E-453.045
GATACGC251.4791383E-553.048
TCGTTAA550.053.045
CGAACAA251.4791383E-553.029
CGTATTA203.13882E-453.051
TTAATCG150.006697003753.024
GTATTAG203.13882E-453.052
CGTATAG700.053.025
CGTATAC251.4791383E-553.042
CGACGTT150.006697003753.044
TTTACGG150.006697003753.035
CCGATTA353.3438482E-853.033
TAGACGC203.13882E-453.031
TAGACCT353.3438482E-853.050
CGCATTA307.013168E-753.040
ATAATCG800.053.042
CGTTGTA150.006697003753.017
TATGGAT251.4791383E-553.017