##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527964_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2033804
Sequences flagged as poor quality	0
Sequence length	59
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33580128665299	31.0	31.0	33.0	30.0	34.0
2	31.9190806980417	31.0	31.0	34.0	30.0	34.0
3	32.16134003079943	33.0	31.0	34.0	30.0	34.0
4	35.8413106671046	37.0	35.0	37.0	35.0	37.0
5	35.699733602648045	37.0	35.0	37.0	33.0	37.0
6	35.691941308011984	37.0	35.0	37.0	33.0	37.0
7	35.42821333815844	37.0	35.0	37.0	32.0	37.0
8	35.317787259735944	37.0	35.0	37.0	32.0	37.0
9	37.44351717274624	39.0	37.0	39.0	34.0	39.0
10	37.626064753535736	39.0	37.0	39.0	35.0	39.0
11	37.756178569813024	39.0	37.0	39.0	35.0	39.0
12	37.86371302249381	39.0	37.0	39.0	35.0	39.0
13	37.89400158520684	39.0	38.0	39.0	35.0	39.0
14	38.29821261045804	40.0	38.0	40.0	35.0	40.0
15	37.55531309801731	40.0	37.0	40.0	32.0	40.0
16	36.98846004826424	39.0	36.0	40.0	32.0	40.0
17	36.88261258213672	39.0	36.0	40.0	31.0	40.0
18	36.88335847505463	39.0	36.0	40.0	31.0	40.0
19	37.11055391768332	39.0	36.0	40.0	32.0	40.0
20	37.23628530576201	39.0	36.0	40.0	32.0	40.0
21	37.23492135918702	40.0	36.0	40.0	32.0	40.0
22	37.20858302963314	40.0	36.0	40.0	32.0	40.0
23	37.10597678045672	39.0	36.0	40.0	32.0	40.0
24	37.07456814914318	39.0	36.0	40.0	31.0	40.0
25	37.01747464357431	39.0	36.0	40.0	31.0	40.0
26	36.83851147898224	39.0	36.0	40.0	31.0	40.0
27	36.681261321149925	39.0	35.0	40.0	30.0	40.0
28	36.67157995559061	39.0	35.0	40.0	30.0	40.0
29	36.62261604363056	39.0	35.0	40.0	30.0	40.0
30	36.59713669557145	39.0	35.0	40.0	30.0	40.0
31	36.56813045898228	39.0	35.0	40.0	30.0	40.0
32	36.42417755103245	39.0	35.0	40.0	30.0	40.0
33	36.28178624882241	39.0	35.0	40.0	30.0	40.0
34	36.17886040149395	39.0	35.0	40.0	30.0	40.0
35	36.02410851783161	38.0	35.0	40.0	30.0	40.0
36	35.80799182222082	38.0	35.0	40.0	29.0	40.0
37	35.6699504967047	38.0	35.0	40.0	28.0	40.0
38	35.47289955177588	38.0	34.0	40.0	28.0	40.0
39	35.28591201512044	38.0	34.0	40.0	27.0	40.0
40	35.10706587262096	38.0	34.0	40.0	27.0	40.0
41	34.89354923089934	37.0	33.0	40.0	26.0	40.0
42	34.7704778828245	37.0	33.0	40.0	26.0	40.0
43	34.52848553744609	37.0	33.0	40.0	25.0	40.0
44	34.361469935155995	36.0	33.0	40.0	25.0	40.0
45	34.11944169644666	36.0	33.0	40.0	24.0	40.0
46	33.90611189672161	36.0	33.0	39.0	23.0	40.0
47	33.72115356248685	35.0	33.0	39.0	23.0	40.0
48	33.49400925556248	35.0	32.0	39.0	23.0	40.0
49	33.38081840728015	35.0	32.0	39.0	23.0	40.0
50	33.13839190010444	35.0	32.0	39.0	23.0	40.0
51	32.936777585254035	35.0	32.0	38.0	22.0	40.0
52	32.77974377078617	35.0	31.0	38.0	22.0	40.0
53	32.52164220347684	35.0	31.0	38.0	21.0	40.0
54	32.288107900269644	35.0	31.0	37.0	20.0	40.0
55	32.0959281228673	35.0	31.0	37.0	20.0	40.0
56	31.838420024741815	35.0	31.0	37.0	20.0	39.0
57	31.6874585751626	34.0	30.0	36.0	20.0	39.0
58	31.454976487409798	34.0	30.0	36.0	20.0	39.0
59	30.875225439619552	34.0	29.0	35.0	18.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	6.0
14	22.0
15	59.0
16	182.0
17	592.0
18	1270.0
19	2389.0
20	4105.0
21	6147.0
22	8917.0
23	12386.0
24	16683.0
25	21749.0
26	27565.0
27	34497.0
28	41683.0
29	49144.0
30	57560.0
31	68743.0
32	84099.0
33	105548.0
34	144935.0
35	194743.0
36	252975.0
37	365022.0
38	439111.0
39	93669.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.128612196652185	22.759813630025313	33.564837122947935	11.546737050374569
2	26.696328653105216	21.699534468414853	34.15343858110221	17.450698297377723
3	19.07111009713817	22.569431469305794	36.28584662042164	22.073611813134402
4	20.300677941433882	25.054479192685235	32.561446432399585	22.0833964334813
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	100.0	0.0
11	99.4794483637558	0.3809609972249047	0.09642030402142979	0.043170334997866065
12	99.36070535803843	0.4117407577131326	0.12341405563171279	0.10413982861672019
13	97.9511300007277	1.0688837272421532	0.429195733708853	0.5507905383212935
14	64.20756375737288	22.48589342925867	5.1302387054013066	8.17630410796714
15	33.73569921192013	26.711325181777596	17.238976813891604	22.313998792410676
16	27.7866500410069	18.929454362367267	28.01582650048874	25.26806909613709
17	22.80052551769984	17.45581186781027	29.815655785906607	29.928006828583285
18	24.037468703965573	19.585023925609352	30.980959817170188	25.396547553254884
19	25.61884036023137	18.746890064135975	28.981553778043505	26.65271579758915
20	28.3725472071055	19.017614283382272	25.294571158282707	27.315267351229515
21	30.351449795555517	18.856438476864042	20.279486125506686	30.51262560207375
22	30.228724105174344	20.289811604264717	21.80480518280031	27.67665910776063
23	29.96876788520428	19.661481637365252	21.382788115275613	28.986962362154863
24	29.576350523452604	21.179671197421186	20.957870079909373	28.286108199216837
25	32.045270832390926	19.448186747592196	22.10212980208516	26.404412617931715
26	32.14469044214683	18.59938322473552	20.875905446149186	28.38002088696846
27	29.839404387050077	22.158379076843197	20.687293367502473	27.31492316860425
28	31.199220770536396	20.19009698083001	20.100265315635134	28.51041693299846
29	29.876379434793126	20.62942151751103	20.95924681041044	28.534952237285406
30	31.106832320125243	19.473902106594345	20.61845684244893	28.800808730831484
31	30.897323439230135	19.325116874585753	20.397344090187648	29.380215595996468
32	31.912121325358783	21.081087459755217	19.304957606534355	27.701833608351638
33	30.666721080300757	22.04179950477037	19.845275159258218	27.446204255670654
34	30.063811458724636	22.24054038638925	20.69122688321982	27.004421271666295
35	31.769924732176747	23.045927729515725	19.183854491386583	26.00029304692094
36	32.22218070177854	23.529700993802745	19.132964631793428	25.11515367262529
37	31.636283535679937	22.633596944444992	19.007042959891905	26.723076559983166
38	30.737180180587707	23.9377049115844	21.022428906620302	24.30268600120759
39	30.66524601190675	24.107927804252522	22.272106850020947	22.954719333819778
40	31.12723743290897	24.087080170950593	20.10282210085141	24.682860295289025
41	31.202908441521405	25.089536651516077	19.062308855720612	24.64524605124191
42	33.57014736916635	23.119828656055354	17.639900403382036	25.670123571396257
43	31.99964205006972	24.126612003909916	18.519729531459276	25.354016414561087
44	31.575609055739882	25.145490912595314	18.602431699416464	24.676468332248337
45	29.299971875362623	22.622681438329355	20.531919496667328	27.54542718964069
46	30.176113332454847	20.92915541517275	21.098837449429737	27.79589380294266
47	30.18688133173108	21.14702301696722	22.39891356295887	26.267182088342828
48	28.843929896882887	21.369168317104304	23.126613970667773	26.66028781534504
49	29.963654314771727	20.921681735309793	21.89301427276178	27.221649677156694
50	30.185307925444143	22.13423712412799	20.958607614106377	26.721847336321492
51	28.828195834013503	22.289856839695467	21.56943343606365	27.312513890227375
52	29.32258959073736	22.355054862710467	21.546029017545447	26.776326529006727
53	28.089579920188967	22.074988543635474	21.968537774534813	27.86689376164075
54	27.58451650208181	22.937313526770524	22.904567008423623	26.573602962724035
55	28.537017333037006	22.877179905241608	21.44306924364393	27.142733518077456
56	29.492124118154944	22.760797008954647	21.21502366993083	26.532055202959576
57	29.450969709962216	21.96794774717721	21.718562850697513	26.862519692163062
58	28.555554025855002	23.365132529978307	20.904030083528205	27.175283360638485
59	28.91773248552958	23.25941929507465	21.99218803778535	25.83066018161042
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	11.0
9	62.0
10	104.0
11	183.5
12	263.0
13	341.5
14	525.5
15	631.0
16	763.0
17	895.0
18	974.5
19	1081.5
20	1109.0
21	1354.0
22	1599.0
23	2304.5
24	3010.0
25	4332.0
26	6975.0
27	8296.0
28	8963.5
29	9631.0
30	10478.5
31	12688.5
32	14051.0
33	14153.0
34	14255.0
35	15719.0
36	18358.0
37	19533.0
38	20527.0
39	21521.0
40	24642.5
41	27764.0
42	31100.5
43	38233.5
44	42030.0
45	43557.0
46	45084.0
47	46599.5
48	52910.0
49	57705.0
50	68078.0
51	78451.0
52	87438.0
53	102876.0
54	109327.0
55	121870.0
56	134413.0
57	150607.0
58	171776.0
59	176751.0
60	176089.5
61	175428.0
62	162242.0
63	149056.0
64	143101.5
65	122259.5
66	107372.0
67	98043.5
68	88715.0
69	79220.5
70	65017.0
71	60308.0
72	51733.5
73	43159.0
74	34980.5
75	21513.5
76	16225.0
77	10985.5
78	5746.0
79	5159.5
80	4573.0
81	2854.0
82	765.5
83	396.0
84	262.0
85	128.0
86	73.0
87	11.0
88	4.0
89	4.0
90	4.0
91	3.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	2033804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.142506864332095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.69953472106023	11.695787374696646
2	6.5634106369374665	1.856461599726368
3	2.684594390284899	1.1390068377765485
4	1.5599964162487128	0.8824904010052355
5	0.987230463628791	0.6980956804273966
6	0.6120708681918214	0.5193729872916324
7	0.43319584747694106	0.4288532672579959
8	0.31607692851313346	0.35760961049231954
9	0.22546282116871802	0.28697485464272465
>10	1.545584279561	4.42179871217754
>50	0.44949961038869546	4.780689706235975
>100	1.7715367972762273	57.555442418884404
>500	0.14136083720403858	12.877928373294647
>1k	0.010445382059411718	2.4994881760905616
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAACAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT	3875	0.19052966755891915	No Hit
GCCAAGGACTGGGGACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCTA	3776	0.1856619418587042	No Hit
ACAAAGGACTGGGGGACACAGGATCTGGAGCTGTGCTGATAACAGCGGAATCCCCTGTC	3746	0.18418687346469964	No Hit
GGGCAGGACTGGGGAAGGAAAGAAAGAAAGAAAGAAAGGAAGTGAAAGAAAGAAAGAAA	3537	0.1739105636531347	No Hit
GCTCAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT	2674	0.13147776285227092	No Hit
CCTGAGGACTGGGACACAGGATCTGGAGCTGGTGCTGATAACAGCGGAATCCCCTGTCT	2256	0.11092514322914107	No Hit
TTATAGGACTGGGGGACAACCAATGACCTTGACACTGCCTCTTAGGCACTTAGCTCTAG	2087	0.10261559127624884	No Hit
TAATAGGACTGGGGATGTGGATTGTCTCAAAGCCTGCCAGGAGCAAATCGAAGCTCTGC	2075	0.10202556391864703	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.002851798895075435	0.0	0.0	0.0	0.0
12	0.006981990398288134	0.0	0.0	0.0	0.0
13	0.009735451400429933	0.0	0.0	0.0	0.0
14	0.038745129815852465	0.0	0.0	0.0	0.0
15	0.06706644298073954	0.0	0.0	0.0	0.0
16	0.08383305372592442	0.0	0.0	0.0	0.0
17	0.09627279718202934	0.0	0.0	0.0	0.0
18	0.11264605635547968	0.0	0.0	0.0	0.0
19	0.12877347079659593	0.0	0.0	0.0	0.0
20	0.14804769781158852	0.0	0.0	0.0	0.0
21	0.16707608009424704	0.0	0.0	0.0	0.0
22	0.18821872707497872	0.0	0.0	0.0	0.0
23	0.20705043357176994	0.0	0.0	0.0	0.0
24	0.22834058739190208	0.0	0.0	0.0	0.0
25	0.24967991015850102	0.0	0.0	0.0	0.0
26	0.27628031019704946	0.0	0.0	0.0	0.0
27	0.3041591028437352	0.0	0.0	0.0	0.0
28	0.3309561786681509	0.0	0.0	0.0	0.0
29	0.36237513546044753	0.0	0.0	0.0	0.0
30	0.40185779947330225	0.0	0.0	0.0	0.0
31	0.43883284721634924	0.0	0.0	0.0	0.0
32	0.4751686986553276	0.0	0.0	0.0	0.0
33	0.5019657744797433	0.0	0.0	0.0	0.0
34	0.5278778092677564	0.0	0.0	0.0	0.0
35	0.5514789035718289	0.0	0.0	0.0	0.0
36	0.5764567283769724	0.0	0.0	0.0	0.0
37	0.6000086537345781	0.0	0.0	0.0	0.0
38	0.6246914648609208	0.0	0.0	0.0	0.0
39	0.6498167965054646	0.0	0.0	0.0	0.0
40	0.6770072239016149	0.0	0.0	0.0	0.0
41	0.7048368476018338	0.0	0.0	0.0	0.0
42	0.7274053940301032	0.0	0.0	0.0	0.0
43	0.7516456846382444	0.0	0.0	0.0	0.0
44	0.7731816831907106	0.0	0.0	0.0	0.0
45	0.7959469054048472	0.0	0.0	0.0	0.0
46	0.8184662828866498	0.0	0.0	0.0	0.0
47	0.8396580988138483	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	50	3.6379788E-12	53.0	20
AGGTATA	15	0.0066970037	53.0	30
TGATACG	20	3.13882E-4	53.0	45
GATACGC	25	1.4791383E-5	53.0	48
TCGTTAA	55	0.0	53.0	45
CGAACAA	25	1.4791383E-5	53.0	29
CGTATTA	20	3.13882E-4	53.0	51
TTAATCG	15	0.0066970037	53.0	24
GTATTAG	20	3.13882E-4	53.0	52
CGTATAG	70	0.0	53.0	25
CGTATAC	25	1.4791383E-5	53.0	42
CGACGTT	15	0.0066970037	53.0	44
TTTACGG	15	0.0066970037	53.0	35
CCGATTA	35	3.3438482E-8	53.0	33
TAGACGC	20	3.13882E-4	53.0	31
TAGACCT	35	3.3438482E-8	53.0	50
CGCATTA	30	7.013168E-7	53.0	40
ATAATCG	80	0.0	53.0	42
CGTTGTA	15	0.0066970037	53.0	17
TATGGAT	25	1.4791383E-5	53.0	17
>>END_MODULE