FastQCFastQC Report
Fri 17 Jun 2016
SRR1527963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784798
Sequences flagged as poor quality0
Sequence length52
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGTTTGGGCCGAGGCAGGCAGATCATGAGGTCGGGAGATCGAGACC17930.22846643340069675No Hit
TTCCAAAGTTGGGTGGTATCAAGCAGAGTAGAAAAAGTTGGGACACAGGATC17350.22107599662588334No Hit
GCATAAAGTTGGGGTTTGAGACCAGCCTGGCCAACGTGGAGAAACCCCATCT12880.16411866493033875No Hit
TTGCAAAGTTGGGAGCCACCACCGTGCCATGGAGCCAGGCAGAGGCTGAGCG11860.1511216899125635No Hit
TAAAAAAGTTGGGCTCGTGGATGAAGTTTCCTTTGGCATGTGGAAACTCGTT11610.14793615681997152No Hit
GAAAAAAGTTGGGGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATC11490.14640710093552736No Hit
GCGCAAAGTTGGGGCTTTTCCTCAGCTGCCGTGCCGGCAGTCTACAGCATGG10830.13799729357108453No Hit
ATTTAAAGTTGGGATTACTGCTTGGGCTCCAAGGGCTGGAAGTGGTGATGGG10600.1350666031258999No Hit
CTGCAAAGTTGGGCTAGCACGATGAAGATTAAGATCATTGCTCTCCCTGAGC9840.1253825825244203No Hit
GCCCAAAGTTGGGCAGCCAGCCCGTGCACCTACGACGCCTGCAGGAGCAGAA9470.12066799354738417No Hit
GCACAAAGTTGGGGGCCACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTC9360.1192663589866437No Hit
CCTTAAAGTTGGGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCC9140.11646308986516275No Hit
GACGAAAGTTGGGTATATCCAGAATCTACAAGGAACTCAAACAAATTTACAA9070.115571140599237No Hit
GTGGAAAGTTGGCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCC8930.1137872420673855No Hit
GCCCAAAGTTGGGGTGCCCATGCTGGAGTGCAATAGTGTGATCTCGGCTCAC8800.11213076485923766No Hit
TGCTAAAGTTGGGGGCAGTGTGACAACTAAAGATGATATCAAGGTTCAGGGC8460.10779843985331257No Hit
CTGCAAAGTTGGGCAACCTGGCCTCCAGGATCCTCAGCCTATGTGCTTTCTT8300.1057596986740537No Hit
CCTGAAAGTTGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGG8010.10206448028664701No Hit
AAGAAAAGTTGGGAGCGCCCGCGATCCAGGCGCGCCTTTGCCGGGAGGGTGG7940.10117253102072125No Hit
CGCCAAAGTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAG7870.1002805817547955No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTTC351.01901605E-746.00000422
ATTTACG750.046.00000430
CGTGGTT351.01901605E-746.00000421
TAGAGGA750.046.00000424
TAGAGAC700.046.00000442
CTGTCCG700.046.00000436
ATCTCGA351.01901605E-746.00000444
GTCGCCG750.046.00000432
CCCCTAC351.01901605E-746.00000421
TCGCACT700.046.00000425
GCGACAT700.046.00000441
TCCGCAC700.046.00000439
CGAGTCA351.01901605E-746.00000441
CAACGAC351.01901605E-746.00000436
CGCGGCA351.01901605E-746.00000435
CGTGCCA1150.046.00000423
TCACAGG700.046.00000446
TCCGACG351.01901605E-746.00000445
CCGGCAT750.046.00000443
CACATTC351.01901605E-746.00000443
Produced by FastQC (version 0.11.5)