FastQCFastQC Report
Fri 17 Jun 2016
SRR1527962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778416
Sequences flagged as poor quality0
Sequence length52
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGTTTGGGCCGAGGCAGGCAGATCATGAGGTCGGGAGATCGAGACC16930.2174929600624859No Hit
TTCCAAAGTTGGGTGGTATCAAGCAGAGTAGAAAAAGTTGGGACACAGGATC15630.20079237836837885No Hit
GCATAAAGTTGGGGTTTGAGACCAGCCTGGCCAACGTGGAGAAACCCCATCT12680.1628949045240591No Hit
GAAAAAAGTTGGGGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATC11100.14259727446506754No Hit
TTGCAAAGTTGGGAGCCACCACCGTGCCATGGAGCCAGGCAGAGGCTGAGCG11100.14259727446506754No Hit
GCGCAAAGTTGGGGCTTTTCCTCAGCTGCCGTGCCGGCAGTCTACAGCATGG10410.13373311956588765No Hit
ATTTAAAGTTGGGATTACTGCTTGGGCTCCAAGGGCTGGAAGTGGTGATGGG10360.1330907895007297No Hit
TAAAAAAGTTGGGCTCGTGGATGAAGTTTCCTTTGGCATGTGGAAACTCGTT10270.13193459538344535No Hit
GCACAAAGTTGGGGGCCACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTC8870.11394935355902241No Hit
CCTTAAAGTTGGGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCC8630.1108661692462642No Hit
GCCCAAAGTTGGGCAGCCAGCCCGTGCACCTACGACGCCTGCAGGAGCAGAA8480.10893917905079031No Hit
CTGCAAAGTTGGGCTAGCACGATGAAGATTAAGATCATTGCTCTCCCTGAGC8430.10829684898563235No Hit
GACGAAAGTTGGGTATATCCAGAATCTACAAGGAACTCAAACAAATTTACAA8250.1059844607510637No Hit
GTGGAAAGTTGGCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCC8100.10405747055558981No Hit
CGCCAAAGTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAG8000.10277281042527389No Hit
CTGCAAAGTTGGGCAACCTGGCCTCCAGGATCCTCAGCCTATGTGCTTTCTT7890.10135968428192636No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAC206.310192E-446.00000425
CTTAAGT206.310192E-446.00000434
ATTTACA800.046.00000445
ATCCTGT351.01899786E-746.00000425
AGGTAGA750.046.00000433
GACAGGC351.01899786E-746.00000429
CAGCGAA206.310192E-446.00000435
AGCAACT206.310192E-446.00000419
ACAGTTA700.046.00000441
GACAGAC405.6079443E-946.00000423
CGGCGAG700.046.00000419
ACCGGCT206.310192E-446.00000441
ACTGCAA351.01899786E-746.00000426
GATGTCA206.310192E-446.00000440
TACTTAT206.310192E-446.00000423
ATCTAAT405.6079443E-946.00000444
GTACAAT700.046.00000431
TTTACCG351.01899786E-746.00000415
ACCATTG750.046.00000415
TCTCTAC206.310192E-446.00000436
Produced by FastQC (version 0.11.5)