##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	778416
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.566983463854804	31.0	31.0	33.0	28.0	34.0
2	31.5378203942365	31.0	31.0	34.0	28.0	34.0
3	31.998663953464472	33.0	31.0	34.0	30.0	34.0
4	35.75727246099772	37.0	35.0	37.0	35.0	37.0
5	35.773305019424065	37.0	35.0	37.0	35.0	37.0
6	36.03583430967503	37.0	35.0	37.0	35.0	37.0
7	35.8123856652484	37.0	35.0	37.0	33.0	37.0
8	35.788091457524	37.0	35.0	37.0	33.0	37.0
9	36.38931239851185	37.0	35.0	39.0	32.0	39.0
10	37.42709939158496	39.0	37.0	39.0	35.0	39.0
11	37.94100326817537	39.0	37.0	39.0	35.0	39.0
12	38.19038534665269	39.0	39.0	39.0	37.0	39.0
13	38.11288308565086	39.0	39.0	39.0	37.0	39.0
14	38.56227646913732	40.0	39.0	40.0	36.0	40.0
15	37.696108507533246	40.0	37.0	40.0	33.0	40.0
16	37.2773915232986	39.0	36.0	40.0	33.0	40.0
17	37.28490293108055	39.0	36.0	40.0	33.0	40.0
18	37.129003000966065	39.0	36.0	40.0	32.0	40.0
19	37.385356929970605	39.0	37.0	40.0	32.0	40.0
20	37.29936049618713	39.0	36.0	40.0	32.0	40.0
21	37.46677483504964	39.0	37.0	40.0	33.0	40.0
22	37.465993761690406	40.0	37.0	40.0	33.0	40.0
23	37.20817275081704	39.0	36.0	40.0	32.0	40.0
24	37.453380968530965	40.0	37.0	40.0	33.0	40.0
25	37.52372381862654	40.0	37.0	40.0	33.0	40.0
26	37.44404919734435	40.0	37.0	40.0	33.0	40.0
27	37.30830558467452	40.0	37.0	40.0	32.0	40.0
28	36.90709723335594	39.0	36.0	40.0	31.0	40.0
29	36.8530875521572	39.0	35.0	40.0	32.0	40.0
30	36.77325620233911	39.0	35.0	40.0	31.0	40.0
31	36.71240056730591	39.0	35.0	40.0	31.0	40.0
32	36.57689333210006	39.0	35.0	40.0	31.0	40.0
33	36.46177622248258	39.0	35.0	40.0	31.0	40.0
34	36.31558318431276	39.0	35.0	40.0	30.0	40.0
35	36.047808626749706	39.0	35.0	40.0	30.0	40.0
36	35.351589638445255	38.0	35.0	40.0	26.0	40.0
37	35.369536340465764	38.0	35.0	40.0	27.0	40.0
38	35.20288123574027	38.0	35.0	40.0	26.0	40.0
39	34.924983556350334	38.0	35.0	40.0	24.0	40.0
40	34.50167262748967	37.0	34.0	40.0	23.0	40.0
41	34.049725339664136	37.0	33.0	40.0	21.0	40.0
42	33.38126143347516	36.0	33.0	40.0	17.0	40.0
43	32.86292804875542	35.0	33.0	40.0	15.0	40.0
44	32.41929508129329	35.0	32.0	40.0	11.0	40.0
45	31.683969753961893	35.0	31.0	40.0	10.0	40.0
46	31.468644015539248	35.0	30.0	39.0	10.0	40.0
47	31.17221382910937	35.0	29.0	39.0	9.0	40.0
48	30.78906522990278	35.0	27.0	39.0	9.0	40.0
49	30.415969096215907	35.0	26.0	39.0	8.0	40.0
50	29.90371215391256	35.0	24.0	39.0	8.0	40.0
51	29.52386384658075	35.0	23.0	39.0	8.0	40.0
52	28.814402581653	35.0	20.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	18.0
16	52.0
17	177.0
18	388.0
19	731.0
20	1326.0
21	2117.0
22	3138.0
23	4533.0
24	6425.0
25	8676.0
26	11707.0
27	15439.0
28	19681.0
29	24886.0
30	30064.0
31	32329.0
32	32163.0
33	35241.0
34	44584.0
35	60107.0
36	75369.0
37	133795.0
38	177433.0
39	58032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94378841133789	16.23039608641138	36.3439343487287	6.481881153522025
2	32.54249655711085	17.25504100635136	31.79829294361884	18.404169492918953
3	19.442303344227252	25.145295060738732	34.35887751536453	21.053524079669483
4	17.644293025837086	28.879930525580157	34.81506032763972	18.660716120943043
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	98.393789439066	0.5607541468829007	0.9634950977369426	0.08196131631415593
12	98.74437318862923	0.8684302480935644	0.2221177365316232	0.16507882674559618
13	96.39344515015108	2.0748545764732484	0.6264002795420444	0.9052999938336314
14	46.12199646461532	37.504624776469136	8.613646173768268	7.759732585147273
15	28.2689461676019	39.94881914040821	19.851981459784998	11.930253232204887
16	41.79230642741156	25.11189389735052	18.514136400073998	14.581663275163923
17	25.790579844196422	25.971716922570963	20.317156892972395	27.920546340260223
18	18.995370084890343	42.68732914020267	22.695448192226266	15.621852582680726
19	28.544377299541633	35.38313703726542	23.765184682740333	12.307300980452611
20	20.74032393989846	33.7170613142587	30.443104972148564	15.099509773694272
21	28.305687447328935	36.97611559885717	15.948413187807034	18.769783766006864
22	26.630362171383936	41.1650068857783	17.431167910217674	14.773463032620091
23	16.959954574417793	40.23953772789871	26.182015785903683	16.61849191177982
24	19.066540256109842	46.5810055291772	15.610419107520915	18.74203510719204
25	21.691871698423466	37.41803868368584	23.632350825265668	17.257738792625023
26	18.670736469959508	38.48841750426507	16.549248730755792	26.29159729501963
27	20.08835892376313	48.88748432714641	15.667714949333003	15.356441799757455
28	19.862001808801462	48.40316745801731	14.583847197385461	17.15098353579577
29	22.39843477009722	42.803205483957164	14.034141127623275	20.764218618322335
30	28.402679287167786	43.2282995210787	13.206177673634665	15.162843518118846
31	20.09246983618014	44.71221043760663	14.66375305749111	20.531566668722125
32	19.426887422663462	52.82817927689051	15.89548519043802	11.849448110008016
33	22.6484296314567	51.73416270991347	13.631400176769235	11.986007481860598
34	19.502553904339067	55.04678732194611	12.211850732770138	13.238808040944688
35	21.202930052825224	52.345403999917785	13.483535795769871	12.968130151487124
36	19.957195124457876	49.99910073790878	17.22279603708043	12.82090810055292
37	19.538781320013975	51.41274074530842	15.915525888470947	13.132952046206656
38	18.25052414133317	52.486459682226474	16.957899118209284	12.305117058231074
39	18.96787835810158	50.795975416743744	18.235493617808473	12.0006526073462
40	20.170962570142443	51.0231033277836	15.863754085219215	12.94218001685474
41	19.32758319459004	54.54769686131837	13.59478736305523	12.529932581036359
42	20.70872130069269	51.76692154323652	14.110706871390105	13.413650284680683
43	21.85951984542969	50.336580954142775	14.259470514480688	13.544428685946846
44	23.889411317341885	47.365290538735074	16.379930525580153	12.36536761834289
45	21.732210026515386	44.8840979630429	19.248062732523483	14.135629277918236
46	22.38854288709379	45.68713901050338	17.104735771104394	14.819582331298431
47	24.847253910505437	45.05932560481799	14.69214404637109	15.40127643830548
48	25.33735175022096	44.69987770035559	15.035405233191506	14.927365316231938
49	23.592397895212844	45.85658568169205	15.030523524696306	15.5204928983988
50	21.617489876878174	47.78974224579146	15.412581447452261	15.180186429878113
51	21.84538858399622	46.998520071529875	15.87159051201414	15.284500832459763
52	23.486927298513905	44.97800661856899	14.987230478304658	16.547835604612445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	53.0
4	104.0
5	84.5
6	65.0
7	114.0
8	163.0
9	427.0
10	691.0
11	1090.0
12	1489.0
13	1767.5
14	2396.0
15	2746.0
16	3573.5
17	4401.0
18	6480.0
19	8559.0
20	12811.5
21	17064.0
22	24638.5
23	32213.0
24	39800.0
25	47387.0
26	49271.0
27	51155.0
28	47315.0
29	43475.0
30	38927.5
31	34380.0
32	32914.0
33	31448.0
34	31466.0
35	31484.0
36	30679.0
37	29874.0
38	30806.0
39	34945.0
40	38152.0
41	40772.0
42	43392.0
43	44895.5
44	46399.0
45	47719.5
46	49040.0
47	47716.5
48	46393.0
49	44001.0
50	41609.0
51	35036.0
52	28463.0
53	26708.0
54	24953.0
55	22282.0
56	19611.0
57	21710.0
58	23809.0
59	18852.0
60	13895.0
61	14573.5
62	15252.0
63	10571.0
64	5112.5
65	4335.0
66	3552.5
67	2770.0
68	2515.0
69	2260.0
70	1500.5
71	741.0
72	505.5
73	270.0
74	381.5
75	493.0
76	329.0
77	165.0
78	98.5
79	32.0
80	19.0
81	6.0
82	4.0
83	2.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	778416.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.54801459634612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17186076728863	34.96249531335518
2	6.969005699502993	5.930347124489454
3	2.8468045140869056	3.6337764005494115
4	1.7070100804664046	2.905195592791782
5	1.1586861650332438	2.464989793120939
6	0.7904868586063457	2.0180187838921557
7	0.6266494710486159	1.8663883588678358
8	0.4933116335761918	1.6791544468757726
9	0.39453460862565093	1.510799785791111
>10	2.547459805214439	18.83429534159192
>50	0.11420049969856565	3.426522362953851
>100	0.16074959753335796	14.887301526133633
>500	0.01675768005173947	4.580403458461798
>1k	0.002482619266924366	1.3003117111251594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATAAAGTTTGGGCCGAGGCAGGCAGATCATGAGGTCGGGAGATCGAGACC	1693	0.2174929600624859	No Hit
TTCCAAAGTTGGGTGGTATCAAGCAGAGTAGAAAAAGTTGGGACACAGGATC	1563	0.20079237836837885	No Hit
GCATAAAGTTGGGGTTTGAGACCAGCCTGGCCAACGTGGAGAAACCCCATCT	1268	0.1628949045240591	No Hit
GAAAAAAGTTGGGGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATC	1110	0.14259727446506754	No Hit
TTGCAAAGTTGGGAGCCACCACCGTGCCATGGAGCCAGGCAGAGGCTGAGCG	1110	0.14259727446506754	No Hit
GCGCAAAGTTGGGGCTTTTCCTCAGCTGCCGTGCCGGCAGTCTACAGCATGG	1041	0.13373311956588765	No Hit
ATTTAAAGTTGGGATTACTGCTTGGGCTCCAAGGGCTGGAAGTGGTGATGGG	1036	0.1330907895007297	No Hit
TAAAAAAGTTGGGCTCGTGGATGAAGTTTCCTTTGGCATGTGGAAACTCGTT	1027	0.13193459538344535	No Hit
GCACAAAGTTGGGGGCCACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTC	887	0.11394935355902241	No Hit
CCTTAAAGTTGGGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCC	863	0.1108661692462642	No Hit
GCCCAAAGTTGGGCAGCCAGCCCGTGCACCTACGACGCCTGCAGGAGCAGAA	848	0.10893917905079031	No Hit
CTGCAAAGTTGGGCTAGCACGATGAAGATTAAGATCATTGCTCTCCCTGAGC	843	0.10829684898563235	No Hit
GACGAAAGTTGGGTATATCCAGAATCTACAAGGAACTCAAACAAATTTACAA	825	0.1059844607510637	No Hit
GTGGAAAGTTGGCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCC	810	0.10405747055558981	No Hit
CGCCAAAGTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAG	800	0.10277281042527389	No Hit
CTGCAAAGTTGGGCAACCTGGCCTCCAGGATCCTCAGCCTATGTGCTTTCTT	789	0.10135968428192636	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.011561941172843312	0.0	0.0	0.0	0.0
12	0.03481428953156153	0.0	0.0	0.0	0.0
13	0.08889848101786191	0.0	0.0	0.0	0.0
14	0.35572239008447926	0.0	0.0	0.0	0.0
15	0.6618568991387639	0.0	0.0	0.0	0.0
16	0.8155022507245483	0.0	0.0	0.0	0.0
17	0.9196881872931697	0.0	0.0	0.0	0.0
18	1.0974851493288935	0.0	0.0	0.0	0.0
19	1.2439364041849088	0.0	0.0	0.0	0.0
20	1.382551232245997	0.0	0.0	0.0	0.0
21	1.5386374380793817	0.0	0.0	0.0	0.0
22	1.67159976156708	0.0	0.0	0.0	0.0
23	1.8105999876672627	0.0	0.0	0.0	0.0
24	2.0009866189800825	0.0	0.0	0.0	0.0
25	2.1805821051982486	0.0	0.0	0.0	0.0
26	2.410793200550862	0.0	0.0	0.0	0.0
27	2.699199399806787	0.0	0.0	0.0	0.0
28	2.979769172267785	0.0	0.0	0.0	0.0
29	3.263293663028509	0.0	0.0	0.0	0.0
30	3.583816365542332	0.0	0.0	0.0	0.0
31	3.897144971326386	0.0	0.0	0.0	0.0
32	4.280487554212658	0.0	0.0	0.0	0.0
33	4.576730180263509	0.0	0.0	0.0	0.0
34	4.805656615485807	0.0	0.0	0.0	0.0
35	5.016083944831555	0.0	0.0	0.0	0.0
36	5.2391009434544	0.0	0.0	0.0	0.0
37	5.467128116585476	0.0	0.0	0.0	0.0
38	5.686034202791309	0.0	0.0	0.0	0.0
39	5.930119627551335	0.0	0.0	0.0	0.0
40	6.191034100018499	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTAC	20	6.310192E-4	46.000004	25
CTTAAGT	20	6.310192E-4	46.000004	34
ATTTACA	80	0.0	46.000004	45
ATCCTGT	35	1.01899786E-7	46.000004	25
AGGTAGA	75	0.0	46.000004	33
GACAGGC	35	1.01899786E-7	46.000004	29
CAGCGAA	20	6.310192E-4	46.000004	35
AGCAACT	20	6.310192E-4	46.000004	19
ACAGTTA	70	0.0	46.000004	41
GACAGAC	40	5.6079443E-9	46.000004	23
CGGCGAG	70	0.0	46.000004	19
ACCGGCT	20	6.310192E-4	46.000004	41
ACTGCAA	35	1.01899786E-7	46.000004	26
GATGTCA	20	6.310192E-4	46.000004	40
TACTTAT	20	6.310192E-4	46.000004	23
ATCTAAT	40	5.6079443E-9	46.000004	44
GTACAAT	70	0.0	46.000004	31
TTTACCG	35	1.01899786E-7	46.000004	15
ACCATTG	75	0.0	46.000004	15
TCTCTAC	20	6.310192E-4	46.000004	36
>>END_MODULE