FastQCFastQC Report
Fri 17 Jun 2016
SRR1527961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805854
Sequences flagged as poor quality0
Sequence length59
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGTTTGGGCCGAGGCAGGCAGATCATGAGGTCGGGAGATCGAGACCATCCTGG16730.20760584423481176No Hit
TTCCAAAGTTGGGTGGTATCAAGCAGAGTAGAAAAAGTTGGGACACAGGATCTGGAGCT15940.19780257962360429No Hit
GCATAAAGTTGGGGTTTGAGACCAGCCTGGCCAACGTGGAGAAACCCCATCTCTACTAA12680.1573486016077354No Hit
TTGCAAAGTTGGGAGCCACCACCGTGCCATGGAGCCAGGCAGAGGCTGAGCGACATGAG11830.14680078525390455No Hit
TAAAAAAGTTGGGCTCGTGGATGAAGTTTCCTTTGGCATGTGGAAACTCGTTGTTTGAA11340.14072027935581383No Hit
ATTTAAAGTTGGGATTACTGCTTGGGCTCCAAGGGCTGGAAGTGGTGATGGGCAGATGT10840.1345156815006192No Hit
GAAAAAAGTTGGGGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATT10530.13066883083039854No Hit
GCACAAAGTTGGGGGCCACTTGGAGCAGCTCTGCCGCGGGGACTGCACTGTCCGCCCTG10520.13054473887329465No Hit
GCGCAAAGTTGGGGCTTTTCCTCAGCTGCCGTGCCGGCAGTCTACAGCATGGCTTCCGT10290.12769062385990515No Hit
CTGCAAAGTTGGGCTAGCACGATGAAGATTAAGATCATTGCTCTCCCTGAGCGCAAGTA9700.12036919839077549No Hit
GACGAAAGTTGGGTATATCCAGAATCTACAAGGAACTCAAACAAATTTACAAGAAAAAA9470.11751508337738599No Hit
CCTTAAAGTTGGGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA8450.10485770375278897No Hit
CTGCAAAGTTGGGCAACCTGGCCTCCAGGATCCTCAGCCTATGTGCTTTCTTTTTTTGT8340.10349269222464615No Hit
GTGGAAAGTTGGCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCC8120.10076266916836053No Hit
GCCCAAAGTTGGGCAGCCAGCCCGTGCACCTACGACGCCTGCAGGAGCAGAAGTGAGGG8120.10076266916836053No Hit
GCCCAAAGTTGGGGTGCCCATGCTGGAGTGCAATAGTGTGATCTCGGCTCACTGCAACC8060.10001811742573717No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATG750.053.00000424
TGTTTAC457.6397555E-1153.00000448
CTCACTT457.6397555E-1153.00000426
ATCTAAT457.6397555E-1153.00000444
GTTTCGC457.6397555E-1153.00000448
CGTGCCA1750.053.00000423
GACGTAG457.6397555E-1153.00000453
CTATAAC750.053.00000416
GGACTAT900.053.00000412
GGTCCCT457.6397555E-1153.00000413
GCCGGAC750.053.00000419
CTAAGGC457.6397555E-1153.00000428
TGACGTA457.6397555E-1153.00000452
TACGGGC900.053.00000450
TGACGGT457.6397555E-1153.00000446
CATTCCG750.053.00000413
TTTCAGG457.6397555E-1153.00000426
CCCGTCA750.053.00000444
TCTACGG900.053.00000448
TGCGATT457.6397555E-1153.00000421