FastQCFastQC Report
Fri 17 Jun 2016
SRR1527960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8166956
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG453.110472E-1046.044
TAACGCG650.046.038
ATTAGCG1050.043.80952519
TAGGGGC1587250.043.7612239
ACGTTAT20000.043.5851
CGTTATG148450.043.521052
TAGGGAT407650.043.5118339
CCGTTAT27400.043.4817541
ACGATAT12450.043.4136541
CTTTATG217300.043.3115542
CGGTATG120350.043.3053632
CATATGT391650.043.2927363
TCGTTAT54900.043.2349741
TAGGGCT323000.043.1873059
TCTTATG211300.043.061052
TTTATGT1193700.043.0269743
CTTATGT537500.043.0089263
TTATGTA3221400.042.9691774
CGTATGT368850.042.9632643
ATATGTA1863350.042.951194
Produced by FastQC (version 0.11.5)