##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527960_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8166956
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.400707191271753	31.0	31.0	33.0	30.0	34.0
2	32.15288364477536	33.0	31.0	34.0	30.0	34.0
3	32.37975115820387	34.0	31.0	34.0	30.0	34.0
4	35.96733274919076	37.0	35.0	37.0	35.0	37.0
5	35.569807649263694	37.0	35.0	37.0	33.0	37.0
6	35.90783077562803	37.0	35.0	37.0	35.0	37.0
7	36.26500828460445	37.0	36.0	37.0	35.0	37.0
8	36.1361715429837	37.0	36.0	37.0	35.0	37.0
9	37.18567419733864	39.0	37.0	39.0	34.0	39.0
10	37.52195432423047	39.0	37.0	39.0	35.0	39.0
11	38.0100296854789	39.0	37.0	39.0	35.0	39.0
12	38.11338226874248	39.0	39.0	39.0	37.0	39.0
13	38.11841217217284	39.0	39.0	39.0	37.0	39.0
14	38.76040424363741	40.0	39.0	40.0	36.0	40.0
15	38.18991641438989	40.0	38.0	40.0	34.0	40.0
16	37.72848659892376	40.0	37.0	40.0	33.0	40.0
17	37.708999166886656	40.0	37.0	40.0	33.0	40.0
18	37.70001356686628	40.0	37.0	40.0	33.0	40.0
19	37.843541339025215	40.0	37.0	40.0	33.0	40.0
20	37.87710696616953	40.0	37.0	40.0	33.0	40.0
21	37.680265939965885	40.0	37.0	40.0	33.0	40.0
22	37.73164420134013	40.0	37.0	40.0	33.0	40.0
23	37.66900054316443	40.0	37.0	40.0	33.0	40.0
24	37.64759758715487	40.0	37.0	40.0	33.0	40.0
25	37.76283489221688	40.0	37.0	40.0	33.0	40.0
26	37.750684832880204	40.0	37.0	40.0	33.0	40.0
27	37.74447260399101	40.0	37.0	40.0	33.0	40.0
28	37.69571404572279	40.0	37.0	40.0	33.0	40.0
29	37.68140369557519	40.0	37.0	40.0	33.0	40.0
30	37.619739349642636	40.0	37.0	40.0	33.0	40.0
31	37.57681393165336	40.0	37.0	40.0	33.0	40.0
32	37.518140908313946	40.0	36.0	40.0	33.0	40.0
33	37.44523075672258	40.0	36.0	40.0	33.0	40.0
34	37.34167479290938	40.0	36.0	40.0	33.0	40.0
35	37.242149584251464	40.0	36.0	40.0	33.0	40.0
36	37.15790191596477	40.0	35.0	40.0	33.0	40.0
37	37.04156689469124	40.0	35.0	40.0	32.0	40.0
38	36.84352713544679	39.0	35.0	40.0	32.0	40.0
39	36.72632532855571	39.0	35.0	40.0	32.0	40.0
40	36.57620072888846	39.0	35.0	40.0	31.0	40.0
41	36.40164940278851	39.0	35.0	40.0	31.0	40.0
42	36.22451008674468	39.0	35.0	40.0	31.0	40.0
43	36.03960875998352	38.0	35.0	40.0	30.0	40.0
44	35.82648124956226	38.0	35.0	40.0	30.0	40.0
45	35.628063258820056	38.0	35.0	40.0	30.0	40.0
46	35.46714614355704	38.0	35.0	40.0	29.0	40.0
47	35.31630561007063	37.0	35.0	40.0	29.0	40.0
48	35.14751750835929	37.0	34.0	40.0	28.0	40.0
49	34.99013034966761	37.0	34.0	40.0	28.0	40.0
50	34.819882952718245	36.0	34.0	40.0	28.0	40.0
51	34.63236963686348	36.0	34.0	40.0	27.0	40.0
52	33.99262822035529	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	9.0
15	35.0
16	115.0
17	410.0
18	1043.0
19	2142.0
20	4054.0
21	7082.0
22	11226.0
23	17273.0
24	25420.0
25	35872.0
26	50534.0
27	67904.0
28	90234.0
29	117885.0
30	150284.0
31	183456.0
32	219461.0
33	274513.0
34	399056.0
35	686940.0
36	712465.0
37	1347400.0
38	2647966.0
39	1114173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.764806863169095	20.047506072029776	35.49644445249858	17.691242612302553
2	27.356887927399143	20.526974799423435	34.671669591461004	17.444467681716418
3	27.80599038368763	20.722984181621648	35.07993675979153	16.39108867489919
4	25.965524976502873	21.28644993312074	36.87366504729547	15.874360043080923
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.54684462607611	0.36955017267143353	0.06882613301700169	0.014779068235460066
12	98.91795670259519	0.9422360056794723	0.08097264145906995	0.058834650266268114
13	98.25523732465315	1.1042057775259229	0.25549298906471396	0.3850639087562122
14	63.63674544101866	22.86141862402589	4.788344641504129	8.713491293451318
15	31.955161751820384	25.144876499885637	17.524019965333473	25.375941782960503
16	28.046190037022363	17.80956086943532	28.9401583650016	25.204090728540717
17	22.7759032863652	17.424937761388698	30.48196904697417	29.317189905271928
18	23.137751691082943	19.346326832175905	32.46552326227789	25.05039821446326
19	24.839952609025932	18.72675694591718	29.581070352283028	26.852220092773855
20	28.15746772726583	18.84724247320544	25.83408065379561	27.161209145733125
21	31.120640297315184	19.731500941109516	21.12619438625603	28.021664375319272
22	29.64535378909841	21.198693858519626	22.814938638092332	26.341013714289634
23	29.15662824680334	21.469100604925508	21.929480212701037	27.44479093557012
24	28.225559192433508	22.89071962674955	22.47330584369501	26.410415337121933
25	31.343342611371973	20.335238735215423	23.0021932284195	25.319225424993107
26	29.788295663647506	20.348266845076672	22.586787537486426	27.276649953789395
27	31.373121148197686	22.511496327395424	20.197549735788954	25.91783278861794
28	31.25412944553638	21.48121037997511	19.743231137770305	27.52142903671821
29	30.570998545847438	20.996635711028684	19.443841744708802	28.98852399841508
30	30.09899894158852	20.76615816223327	20.00498104801838	29.129861848159827
31	29.25978785731183	20.37611075656585	20.9470946090563	29.417006777066018
32	29.636280641159324	22.781009227917966	19.21844564853784	28.364264482384872
33	31.300131897367883	22.176683699532603	20.336903982340544	26.186280420758973
34	29.58178053120404	22.705938418181756	20.619322548082785	27.092958502531424
35	30.84811525861043	22.683592271098313	20.01860913662324	26.449683333668016
36	33.415583480552606	21.914223610363518	20.303268439305906	24.366924469777967
37	33.161449627009134	21.937108513869795	20.53199502972711	24.36944682939396
38	30.817957143395898	24.590483896325633	22.12376312545335	22.467795834825115
39	31.502655334496723	24.69713317911839	22.325013138310037	21.475198348074855
40	31.858712107669003	25.33991856941558	20.06060764867596	22.740761674239458
41	31.385928857704144	25.200294944652573	19.020073084757648	24.393703112885635
42	31.81088523067836	25.895364196892945	18.485859358125598	23.8078912143031
43	32.700482284954155	23.16040394977027	19.076177219517284	25.062936545758298
44	31.090384226387407	24.123835122902584	19.721007435328413	25.064773215381592
45	28.207400652091184	23.8958432003307	20.541153888915282	27.355602258662838
46	28.562649290629217	22.93581598823356	21.04178105036932	27.45975367076791
47	29.44366297553213	20.770333524510235	22.837150096070065	26.94885340388757
48	29.734052687439483	20.558345116589336	22.18684660478151	27.520755591189666
49	29.85500350436564	20.011654280983024	22.340269740647557	27.79307247400378
50	29.797883079081117	21.868539514600055	20.958763583396312	27.374813822922516
51	28.575395777814894	22.574702251365135	22.43329093483545	26.416611035984523
52	29.351094826517983	22.16606774910995	22.52828348775235	25.954553936619718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	5.0
4	8.0
5	30.0
6	52.0
7	383.5
8	715.0
9	1347.5
10	1980.0
11	2860.5
12	3741.0
13	4405.5
14	6104.0
15	7138.0
16	8577.0
17	10016.0
18	12502.0
19	14988.0
20	19211.5
21	23435.0
22	29752.5
23	36070.0
24	43097.5
25	50125.0
26	55097.0
27	60069.0
28	64179.0
29	68289.0
30	69974.5
31	71660.0
32	78300.5
33	84941.0
34	89613.5
35	94286.0
36	103685.5
37	113085.0
38	128804.5
39	162818.0
40	181112.0
41	209498.0
42	237884.0
43	272848.5
44	307813.0
45	328009.0
46	348205.0
47	378280.0
48	408355.0
49	439973.5
50	471592.0
51	518821.5
52	566051.0
53	615882.0
54	665713.0
55	716687.0
56	767661.0
57	784045.0
58	800429.0
59	760561.5
60	720694.0
61	638954.5
62	557215.0
63	487683.0
64	368060.5
65	317970.0
66	274082.5
67	230195.0
68	202153.5
69	174112.0
70	138801.5
71	103491.0
72	80574.0
73	57657.0
74	43263.0
75	28869.0
76	19066.5
77	9264.0
78	6235.0
79	3206.0
80	1968.0
81	730.0
82	459.5
83	189.0
84	153.0
85	117.0
86	102.0
87	87.0
88	43.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	8166956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.578106330509115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.14699832771144	6.360408357431903
2	7.7993599799364866	1.3380747843562524
3	3.695561398953626	0.9510275589344765
4	2.328604700291036	0.7990007488327927
5	1.572901202693893	0.6746256882046943
6	1.084035695434447	0.5579384076902448
7	0.7478727064937563	0.4490732118592354
8	0.5373980867657586	0.3687886344071073
9	0.40058383348634313	0.30926256461359286
>10	2.7439381934111977	4.969921767818289
>50	1.150051874347443	7.423499066351848
>100	3.597925283894341	64.68307556631812
>500	0.18415818202019285	9.975368090279863
>1k	0.010610534559963693	1.1399355529016073
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.025186862767474196	0.0	0.0	0.0	0.0
11	0.025835819367705667	0.0	0.0	0.0	0.0
12	0.031211139131887083	0.0	0.0	0.0	0.0
13	0.03972104171982805	0.0	0.0	0.0	0.0
14	0.060659075425409416	0.0	0.0	0.0	0.0
15	0.09299670525958509	0.0	0.0	0.0	0.0
16	0.11355516057635183	0.0	0.0	0.0	0.0
17	0.131334122529863	0.0	0.0	0.0	0.0
18	0.15637405172747348	0.0	0.0	0.0	0.0
19	0.18176907038558798	0.0	0.0	0.0	0.0
20	0.2051559969222315	0.0	0.0	0.0	0.0
21	0.22599607491457038	0.0	0.0	0.0	0.0
22	0.2493952459153692	0.0	0.0	0.0	0.0
23	0.27502290939243457	0.0	0.0	0.0	0.0
24	0.2997689714503176	0.0	0.0	0.0	0.0
25	0.32866590685685093	0.0	0.0	0.0	0.0
26	0.3622892054273342	0.0	0.0	0.0	0.0
27	0.39647574934896185	0.0	0.0	0.0	0.0
28	0.4318377618294993	0.0	0.0	0.0	0.0
29	0.4696976449977201	0.0	0.0	0.0	0.0
30	0.5121124688317165	0.0	0.0	0.0	0.0
31	0.5507192643134112	0.0	0.0	0.0	0.0
32	0.5856772094768234	0.0	0.0	0.0	0.0
33	0.6172556825333698	0.0	0.0	0.0	0.0
34	0.6471199306081727	0.0	0.0	0.0	0.0
35	0.6742903965688073	0.0	0.0	0.0	0.0
36	0.7008853727141422	0.0	0.0	0.0	0.0
37	0.7256926570928017	0.0	0.0	0.0	0.0
38	0.751589698781284	0.0	0.0	0.0	0.0
39	0.7792009654515097	0.0	0.0	0.0	0.0
40	0.8107427051155902	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAG	45	3.110472E-10	46.0	44
TAACGCG	65	0.0	46.0	38
ATTAGCG	105	0.0	43.809525	19
TAGGGGC	158725	0.0	43.761223	9
ACGTTAT	2000	0.0	43.585	1
CGTTATG	14845	0.0	43.52105	2
TAGGGAT	40765	0.0	43.511833	9
CCGTTAT	2740	0.0	43.481754	1
ACGATAT	1245	0.0	43.413654	1
CTTTATG	21730	0.0	43.311554	2
CGGTATG	12035	0.0	43.305363	2
CATATGT	39165	0.0	43.292736	3
TCGTTAT	5490	0.0	43.234974	1
TAGGGCT	32300	0.0	43.187305	9
TCTTATG	21130	0.0	43.06105	2
TTTATGT	119370	0.0	43.026974	3
CTTATGT	53750	0.0	43.008926	3
TTATGTA	322140	0.0	42.969177	4
CGTATGT	36885	0.0	42.963264	3
ATATGTA	186335	0.0	42.95119	4
>>END_MODULE