FastQCFastQC Report
Fri 17 Jun 2016
SRR1527959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8100837
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAT406100.043.7005659
CCGTTAT26900.043.6914521
TAGGGGC1552250.043.6588829
CGTTATG149850.043.3446772
ACGTTAT22200.043.3063051
TCTTATG205700.043.2829362
CTTTATG211150.043.2768172
CATATGT384850.043.2389263
TAGGGCT318750.043.2075279
CGTATGT361800.043.1774443
CGGTATG117750.043.128662
CTTATGT532850.043.051893
ATATGTA1843950.042.99524
TTTATGT1179400.042.977283
CGCATAT19000.042.9736821
GTATGTA2254800.042.9337434
TAATATG134950.042.9321942
TATATGT604950.042.9166073
TTATGTA3214500.042.8653564
ATGTAGG8567450.042.8504526
Produced by FastQC (version 0.11.5)