##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527959_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8100837
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.341265229753418	31.0	31.0	33.0	30.0	34.0
2	31.982461441947294	33.0	31.0	34.0	30.0	34.0
3	32.30371935640724	34.0	31.0	34.0	30.0	34.0
4	35.93182087233702	37.0	35.0	37.0	35.0	37.0
5	35.54732257913596	37.0	35.0	37.0	33.0	37.0
6	35.89544352021896	37.0	35.0	37.0	35.0	37.0
7	36.267102522862764	37.0	36.0	37.0	35.0	37.0
8	36.085198850439774	37.0	36.0	37.0	35.0	37.0
9	37.123795726293466	39.0	37.0	39.0	34.0	39.0
10	37.49608787338889	39.0	37.0	39.0	35.0	39.0
11	37.981375381334054	39.0	37.0	39.0	35.0	39.0
12	38.07101266202493	39.0	38.0	39.0	37.0	39.0
13	38.120311765315115	39.0	39.0	39.0	37.0	39.0
14	38.78453781998083	40.0	39.0	40.0	36.0	40.0
15	38.20896692526957	40.0	38.0	40.0	34.0	40.0
16	37.763110651405526	40.0	37.0	40.0	33.0	40.0
17	37.755164188589404	40.0	37.0	40.0	33.0	40.0
18	37.76679014773412	40.0	37.0	40.0	33.0	40.0
19	37.90064384704939	40.0	38.0	40.0	33.0	40.0
20	37.911074250722486	40.0	38.0	40.0	34.0	40.0
21	37.90405176650265	40.0	37.0	40.0	34.0	40.0
22	37.852955441517956	40.0	37.0	40.0	34.0	40.0
23	37.76516352569494	40.0	37.0	40.0	33.0	40.0
24	37.74604192134714	40.0	37.0	40.0	33.0	40.0
25	37.70262961716178	40.0	37.0	40.0	33.0	40.0
26	37.68652054102557	40.0	37.0	40.0	33.0	40.0
27	37.71412052853304	40.0	37.0	40.0	33.0	40.0
28	37.51236372241535	40.0	37.0	40.0	33.0	40.0
29	37.60451407675528	40.0	37.0	40.0	33.0	40.0
30	37.60549076595419	40.0	37.0	40.0	33.0	40.0
31	37.60031191838572	40.0	37.0	40.0	33.0	40.0
32	37.53953251991121	40.0	36.0	40.0	33.0	40.0
33	37.461657604022896	40.0	36.0	40.0	33.0	40.0
34	37.37636012179976	40.0	36.0	40.0	33.0	40.0
35	37.20728709885164	40.0	36.0	40.0	33.0	40.0
36	36.75283615754767	40.0	35.0	40.0	31.0	40.0
37	36.8559115558059	39.0	35.0	40.0	32.0	40.0
38	36.77324367840014	39.0	35.0	40.0	31.0	40.0
39	36.68976168758858	39.0	35.0	40.0	31.0	40.0
40	36.55035695694161	39.0	35.0	40.0	31.0	40.0
41	36.40411626601054	39.0	35.0	40.0	31.0	40.0
42	36.08221705979271	39.0	35.0	40.0	30.0	40.0
43	35.90541644030117	38.0	35.0	40.0	30.0	40.0
44	35.7013566622807	38.0	35.0	40.0	30.0	40.0
45	35.26712227390824	38.0	35.0	40.0	28.0	40.0
46	35.262809633128036	37.0	35.0	40.0	28.0	40.0
47	35.19050809194161	37.0	35.0	40.0	28.0	40.0
48	35.01926331809911	37.0	34.0	40.0	28.0	40.0
49	34.87219012060112	37.0	34.0	40.0	27.0	40.0
50	34.63605452127972	36.0	34.0	40.0	27.0	40.0
51	34.5422677928219	36.0	34.0	40.0	27.0	40.0
52	34.04192072991964	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	19.0
15	31.0
16	116.0
17	379.0
18	963.0
19	2168.0
20	4016.0
21	6981.0
22	11225.0
23	17515.0
24	25343.0
25	36621.0
26	50993.0
27	69576.0
28	91814.0
29	120291.0
30	152915.0
31	185597.0
32	222957.0
33	282082.0
34	406676.0
35	682713.0
36	723445.0
37	1361292.0
38	2556523.0
39	1088580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.779430323064148	20.00700668338346	35.47117415151052	17.74238884204188
2	27.502071699504633	20.57919447089233	34.52028475576042	17.398449073842617
3	27.805237903194445	20.74417742265398	35.09848426773678	16.35210040641479
4	25.952737970162836	21.29703387440088	36.85921590571443	15.89101224972185
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	100.0	0.0	0.0
11	99.53641827381541	0.37523283087908077	0.07259743653649617	0.01575145876901362
12	98.91006324408205	0.9475070292119198	0.08608986947891928	0.056339857227098875
13	98.24839087615267	1.1099223450613807	0.2572450229525665	0.3844417558333787
14	63.64144840835583	22.854403810371693	4.7840118249509285	8.72013595632155
15	31.95235257788794	25.162004864435612	17.556235238408078	25.329407319268366
16	28.049817568233998	17.82054126011917	28.93050434171185	25.199136829934982
17	22.768745995012615	17.43779068755488	30.44403930112407	29.34942401630844
18	23.128795209680185	19.369912022671237	32.452807037099994	25.048485730548585
19	24.828619561163865	18.750753780134076	29.570969024558817	26.849657634143238
20	28.11550213885306	18.8744570468459	25.849304213873207	27.160736600427832
21	30.829283443180007	19.812360130193955	21.157134750396782	28.20122167622926
22	29.64150494572351	21.173923139053407	22.86035381282206	26.324218102401026
23	29.16797363037918	21.452128959019916	21.943818891800934	27.436078518799974
24	28.19040797882984	22.937913699535	22.481960814666436	26.389717506968722
25	31.40396480018052	20.30038377515805	22.99459920005797	25.30105222460346
26	29.768257280081055	20.37466005056021	22.5723465365369	27.28473613282183
27	31.350241956479312	22.499588622755894	20.213701374314777	25.936468046450017
28	31.35279724798808	21.494964038901166	19.74546580803934	27.406772905071414
29	30.600553992136863	20.98954712951267	19.434991717522525	28.97490716082795
30	30.12197381579212	20.77540876331668	19.995896720301864	29.106720700589335
31	29.28140635344224	20.352267302749087	20.933540571178018	29.432785772630655
32	29.64101116958655	22.802421527553264	19.19365863058348	28.362908672276703
33	31.314850551862726	22.212223255448787	20.329282023573615	26.14364416911487
34	29.57208002086698	22.742032706003094	20.598081408131037	27.087805864998888
35	30.847367994196155	22.701987461295666	20.02090648163887	26.42973806286931
36	33.491329352757006	21.852050596746977	20.372302269506225	24.28431778098979
37	33.18218845780997	21.953558626102463	20.522323804318	24.341929111769563
38	30.84633106430854	24.61061739669617	22.096580884172834	22.44647065482246
39	31.508583619198856	24.717976673274624	22.304337193798617	21.469102513727904
40	31.875064268050323	25.386450807490636	20.051310747272165	22.687174177186876
41	31.435208485246648	25.22204804268991	18.989568608774622	24.353174863288817
42	31.91847953489251	25.886460868179423	18.516308870305622	23.678750726622447
43	32.63847920900025	23.182456331364275	19.099668342913205	25.079396116722258
44	31.317405843371493	24.047329924055997	19.643118359251023	24.992145873321487
45	28.247599599893196	23.926144915642666	20.575157850972683	27.25109763349145
46	28.58379942714561	22.998364243102287	21.00712555998843	27.410710769763668
47	29.454215656974704	20.780099636617795	22.82244414990698	26.94324055650052
48	29.785712266522584	20.558073196633877	22.161598857006997	27.494615679836542
49	29.906156610730473	20.030584493923282	22.376379132181032	27.686879763165212
50	29.910489496332293	21.885652062867084	20.989880428405115	27.213978012395508
51	28.554086448104066	22.573346433214247	22.44279202260211	26.429775096079577
52	29.377976621428132	22.15703142774012	22.509698689160146	25.955293261671603
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.5
4	11.0
5	37.0
6	63.0
7	368.5
8	674.0
9	1313.5
10	1953.0
11	2893.0
12	3833.0
13	4342.5
14	5972.5
15	7093.0
16	8574.5
17	10056.0
18	12388.5
19	14721.0
20	19034.5
21	23348.0
22	29422.0
23	35496.0
24	42357.0
25	49218.0
26	54249.0
27	59280.0
28	63429.5
29	67579.0
30	69525.0
31	71471.0
32	78099.0
33	84727.0
34	89229.5
35	93732.0
36	102651.0
37	111570.0
38	127003.0
39	161873.0
40	181310.0
41	209137.5
42	236965.0
43	271517.0
44	306069.0
45	326638.0
46	347207.0
47	375815.5
48	404424.0
49	436621.5
50	468819.0
51	515308.5
52	561798.0
53	610505.0
54	659212.0
55	711020.5
56	762829.0
57	776495.0
58	790161.0
59	752080.5
60	714000.0
61	633356.5
62	552713.0
63	483606.0
64	365304.5
65	316110.0
66	272286.5
67	228463.0
68	200412.5
69	172362.0
70	137716.0
71	103070.0
72	80040.0
73	57010.0
74	42698.5
75	28387.0
76	18780.5
77	9174.0
78	6155.0
79	3136.0
80	1886.5
81	637.0
82	399.0
83	161.0
84	132.5
85	104.0
86	100.5
87	97.0
88	51.5
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	8100837.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.866902016616223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52062441403359	6.519001728796783
2	8.22350273813047	1.4583398602475621
3	3.9459026510394977	1.0496379652162025
4	2.322755113650842	0.8238256800534517
5	1.5512611795937938	0.6877440440819335
6	1.061741838414375	0.5648616508897342
7	0.7653729766036503	0.4750541032797328
8	0.5099849853065225	0.36175895157267185
9	0.40836098784012903	0.32588071799283297
>10	2.7586190719979347	5.189680334855543
>50	1.2116769881642702	8.114551478938832
>100	3.554127225256256	64.7047681627234
>500	0.15785436134834668	8.8134128084439
>1k	0.008215468620360019	0.9114825129074359
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.026478745344462554	0.0	0.0	0.0	0.0
11	0.02730582037387001	0.0	0.0	0.0	0.0
12	0.03225592614689075	0.0	0.0	0.0	0.0
13	0.04152657311830864	0.0	0.0	0.0	0.0
14	0.06284535783154259	0.0	0.0	0.0	0.0
15	0.09352120034016238	0.0	0.0	0.0	0.0
16	0.11366726672811711	0.0	0.0	0.0	0.0
17	0.13177650655111317	0.0	0.0	0.0	0.0
18	0.1561443589100731	0.0	0.0	0.0	0.0
19	0.18138866391213648	0.0	0.0	0.0	0.0
20	0.2041393994225535	0.0	0.0	0.0	0.0
21	0.22592727146589914	0.0	0.0	0.0	0.0
22	0.24857925174892423	0.0	0.0	0.0	0.0
23	0.27240395035722853	0.0	0.0	0.0	0.0
24	0.2977963882003798	0.0	0.0	0.0	0.0
25	0.3272131015597524	0.0	0.0	0.0	0.0
26	0.3600245258607228	0.0	0.0	0.0	0.0
27	0.3948233991129559	0.0	0.0	0.0	0.0
28	0.4299926044679087	0.0	0.0	0.0	0.0
29	0.46895154167402703	0.0	0.0	0.0	0.0
30	0.5101818491101598	0.0	0.0	0.0	0.0
31	0.5484124665142627	0.0	0.0	0.0	0.0
32	0.5821744098788805	0.0	0.0	0.0	0.0
33	0.6123317874436925	0.0	0.0	0.0	0.0
34	0.6410078365976256	0.0	0.0	0.0	0.0
35	0.6676840923968721	0.0	0.0	0.0	0.0
36	0.6930888746434474	0.0	0.0	0.0	0.0
37	0.7175184490195272	0.0	0.0	0.0	0.0
38	0.7425035215496868	0.0	0.0	0.0	0.0
39	0.7699451303612207	0.0	0.0	0.0	0.0
40	0.8014850811095199	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGAT	40610	0.0	43.700565	9
CCGTTAT	2690	0.0	43.691452	1
TAGGGGC	155225	0.0	43.658882	9
CGTTATG	14985	0.0	43.344677	2
ACGTTAT	2220	0.0	43.306305	1
TCTTATG	20570	0.0	43.282936	2
CTTTATG	21115	0.0	43.276817	2
CATATGT	38485	0.0	43.238926	3
TAGGGCT	31875	0.0	43.207527	9
CGTATGT	36180	0.0	43.177444	3
CGGTATG	11775	0.0	43.12866	2
CTTATGT	53285	0.0	43.05189	3
ATATGTA	184395	0.0	42.9952	4
TTTATGT	117940	0.0	42.97728	3
CGCATAT	1900	0.0	42.973682	1
GTATGTA	225480	0.0	42.933743	4
TAATATG	13495	0.0	42.932194	2
TATATGT	60495	0.0	42.916607	3
TTATGTA	321450	0.0	42.865356	4
ATGTAGG	856745	0.0	42.850452	6
>>END_MODULE