FastQCFastQC Report
Fri 17 Jun 2016
SRR1527958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8155203
Sequences flagged as poor quality0
Sequence length59
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGC1563600.050.1408589
TAGGGAT416900.050.03799
TAGGGCT322800.049.6751829
CTTTATG214750.049.56952
CATATGT391050.049.5371443
CGTTATG145400.049.5006872
TCTTATG210400.049.4985732
CGGTATG120300.049.4975052
ACGTTAT21900.049.4908641
CGTATGT378150.049.4820823
CTTATGT545800.049.4410973
TTATGTA3214250.049.325414
GTATGTA2282200.049.3109974
TAGGGGA1365450.049.279589
CTCTTAT36050.049.251041
TGTAGGG8495450.049.237487
ATGTAGG8640900.049.229976
TATGTAG8736700.049.2245985
GTAGGGG5403250.049.2216078
TTTATGT1185500.049.173093
Produced by FastQC (version 0.11.5)