FastQCFastQC Report
Fri 17 Jun 2016
SRR1527957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11727621
Sequences flagged as poor quality0
Sequence length52
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA262330.22368560511974253No Hit
CCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA142890.12184056766500212No Hit
AAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121360.10348219813720105No Hit
GGGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA119030.10149543543400662No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAAAT233150.042.152691
ACGACGT451.5905243E-840.8888916
GCAAATA394700.040.3417782
CGTTAAG402.913639E-740.2520
CCAAATA236550.039.8841672
ACCTAAT25350.039.6489141
GGCCAAT61000.039.627871
GCCAAAT99550.039.6233061
CCGTTCG1050.039.42857432
GGCTAAT94200.039.114651
AGCGAAT37050.039.0472341
GCCGAAT37300.038.9705121
GCCTAAT37600.038.8430861
TAAAGGA410550.038.817937
TGGGAAT105950.038.8145371
CCTAATA100000.038.7782
TAAAAGG125150.038.7774667
CCGACGC950.038.7368440
CAAATAA1079700.038.727423
TGCGAAT75800.038.626651