##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11727621
Sequences flagged as poor quality	0
Sequence length	52
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.846253046547123	31.0	30.0	34.0	16.0	34.0
2	31.054248512976333	31.0	30.0	34.0	27.0	34.0
3	31.733160033053593	31.0	31.0	34.0	30.0	34.0
4	35.49243695716292	37.0	35.0	37.0	33.0	37.0
5	35.57638893685258	37.0	35.0	37.0	33.0	37.0
6	35.96954343937274	37.0	35.0	37.0	35.0	37.0
7	36.15832520508635	37.0	35.0	37.0	35.0	37.0
8	36.04773201657864	37.0	35.0	37.0	35.0	37.0
9	37.66284509023612	39.0	37.0	39.0	35.0	39.0
10	37.54947742598435	39.0	37.0	39.0	35.0	39.0
11	38.17931539567999	39.0	39.0	39.0	37.0	39.0
12	38.161454057903136	39.0	39.0	39.0	37.0	39.0
13	37.99301742442052	39.0	38.0	39.0	35.0	39.0
14	38.41797078878999	40.0	38.0	40.0	34.0	40.0
15	37.33366784277903	40.0	36.0	40.0	31.0	40.0
16	37.31358013701159	39.0	36.0	40.0	32.0	40.0
17	37.4379158398792	39.0	37.0	40.0	33.0	40.0
18	37.02612806126665	39.0	36.0	40.0	32.0	40.0
19	37.24769337276503	39.0	36.0	40.0	32.0	40.0
20	37.190960383184276	39.0	36.0	40.0	32.0	40.0
21	37.16614878669766	39.0	37.0	40.0	32.0	40.0
22	37.22461921305267	39.0	36.0	40.0	32.0	40.0
23	37.20785289701978	39.0	36.0	40.0	32.0	40.0
24	37.64070513533819	39.0	37.0	40.0	33.0	40.0
25	37.94346909744099	40.0	38.0	40.0	34.0	40.0
26	37.9375412967387	40.0	38.0	40.0	34.0	40.0
27	37.83463645354842	40.0	38.0	40.0	33.0	40.0
28	37.54421651245381	40.0	37.0	40.0	33.0	40.0
29	37.44356975724232	40.0	37.0	40.0	33.0	40.0
30	37.32537110467673	40.0	36.0	40.0	33.0	40.0
31	37.122751408832194	40.0	36.0	40.0	32.0	40.0
32	37.00501644792239	40.0	36.0	40.0	31.0	40.0
33	36.90708533299294	40.0	36.0	40.0	31.0	40.0
34	36.76536878195501	39.0	35.0	40.0	31.0	40.0
35	36.64361714963333	39.0	35.0	40.0	31.0	40.0
36	36.37170769757993	39.0	35.0	40.0	30.0	40.0
37	36.33882515473513	39.0	35.0	40.0	30.0	40.0
38	36.23776672182704	39.0	35.0	40.0	30.0	40.0
39	36.057095040844175	39.0	35.0	40.0	30.0	40.0
40	35.74951910536672	38.0	35.0	40.0	30.0	40.0
41	35.35263545777954	38.0	35.0	40.0	28.0	40.0
42	34.956815538292034	37.0	35.0	40.0	26.0	40.0
43	34.54451989879277	37.0	34.0	40.0	24.0	40.0
44	34.21217235788912	35.0	33.0	40.0	23.0	40.0
45	33.82020275041289	35.0	33.0	40.0	22.0	40.0
46	33.376063397683126	35.0	33.0	40.0	20.0	40.0
47	32.93162031753925	35.0	33.0	40.0	20.0	40.0
48	32.4370546251452	35.0	32.0	39.0	17.0	40.0
49	31.938114047171204	35.0	31.0	39.0	13.0	40.0
50	31.457955624589165	35.0	30.0	38.0	11.0	40.0
51	30.937692137220328	35.0	28.0	38.0	10.0	40.0
52	30.095165677676658	35.0	27.0	37.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	7.0
13	13.0
14	32.0
15	85.0
16	320.0
17	1055.0
18	2630.0
19	5650.0
20	10883.0
21	18781.0
22	29883.0
23	44634.0
24	63795.0
25	88918.0
26	121825.0
27	156485.0
28	196720.0
29	245972.0
30	304964.0
31	369217.0
32	440630.0
33	544601.0
34	714700.0
35	944832.0
36	1408869.0
37	2606790.0
38	2864391.0
39	540935.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.75064729666827	19.765108371083954	39.78908424820345	6.695160084044326
2	27.93918732537486	20.922683296126298	31.33881969753286	19.799309680965987
3	24.940471729091517	23.586207296432924	30.212146180371963	21.261174794103596
4	20.664736692974646	28.54202058541967	28.382337730729873	22.410904990875814
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	100.0	0.0	0.0
11	98.5206377320686	1.3737312963984767	0.06566549174807064	0.039965479784860036
12	98.33911754140077	1.349335896854102	0.16692217458255174	0.1446243871625797
13	95.46292466306679	3.531457914610303	0.539649089956096	0.4659683323668116
14	33.20705026194145	53.70589653263863	6.115187385404082	6.97186582001584
15	21.72123399963215	49.125709297733955	17.823913306884663	11.329143395749233
16	47.45452636984091	29.214578131404483	11.737700254808711	11.593195243945894
17	31.335451580503836	24.839760766484524	16.616703421776677	27.208084231234963
18	24.998258385055248	39.54486591952451	24.11041420932685	11.346461486093386
19	34.970067671866275	33.61617842186407	24.41472145117923	6.999032455090423
20	25.774673311833663	29.017769247488474	36.696692364120565	8.5108650765573
21	31.36885136380175	29.590519679993072	20.298942129865896	18.741686826339286
22	23.788678027709114	41.77167730778476	21.833072538752745	12.606572125753383
23	10.302780077903268	46.06388627326889	27.590804648274357	16.04252900055348
24	11.680356996529815	48.573150513646375	17.280137207708197	22.466355282115615
25	20.314955607791212	36.433049806094516	20.343904360483684	22.908090225630588
26	15.058697752937276	43.314206692047776	8.448482433052705	33.17861312196225
27	17.845068492578335	55.5465938061948	7.232967368232654	19.37537033299422
28	21.058414148956555	49.82398390943909	9.097548428619923	20.020053512984433
29	24.831165672901605	41.79330999867748	8.409582813087155	24.96594151533376
30	29.223326708801384	43.32340719400806	9.961278591796239	17.491987505394317
31	19.657951088289774	49.304347403450365	12.127549142319657	18.910152365940203
32	20.834737070715363	58.413628816961264	12.066411423084016	8.68522268923936
33	24.139559080226075	56.21243217187868	11.806324573415187	7.841684174480059
34	19.823159360282876	59.06751249891175	12.613981983217226	8.495346157588141
35	21.201102934687267	53.851765844070165	16.089068703703845	8.858062517538723
36	15.878966416121393	57.13517686153057	17.92046315275707	9.065393569590968
37	16.55953070106887	59.76582121813111	13.808435658007706	9.866212422792312
38	15.662537184651516	59.99255944577337	13.759951826546917	10.584951543028206
39	15.391280124076316	61.860952020874485	12.336065430490976	10.411702424558229
40	14.51516893323889	65.06485842269288	11.386853309805971	9.033119334262253
41	14.670170531602276	66.13270500470641	11.001361657236366	8.19576280645495
42	16.080030212436096	63.38155027349537	11.791999417443657	8.746420096624883
43	17.354679180031482	60.71235589895001	12.86410943873442	9.06885548228409
44	18.213284689196556	61.53123468092975	11.00125933469371	9.254221295179986
45	18.48749204975161	60.51471138093566	11.282970348376708	9.714826220936027
46	19.66704074082885	58.86734402484528	11.779976518681837	9.685638715644034
47	22.31010023260472	56.49939574275123	11.553988656352383	9.63651536829166
48	21.48423793708886	56.72971525938637	11.526881709427684	10.259165094097089
49	19.558050179145457	57.800154012480455	12.041112174412866	10.600683633961228
50	17.75339602123909	58.862458123433555	12.580906221304389	10.803239634022962
51	17.504922780161465	57.46372601911334	13.265520773565246	11.76583042715995
52	18.228752446894386	56.52553062552072	12.735583798282704	12.510133129302185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	177.0
1	947.0
2	1717.0
3	4975.0
4	8233.0
5	19380.5
6	30528.0
7	38481.5
8	46435.0
9	75437.5
10	104440.0
11	108000.0
12	111560.0
13	85984.0
14	58986.5
15	57565.0
16	72037.0
17	86509.0
18	139513.0
19	192517.0
20	328166.0
21	463815.0
22	606494.0
23	749173.0
24	776241.0
25	803309.0
26	743948.5
27	684588.0
28	649145.5
29	613703.0
30	614954.0
31	616205.0
32	620692.0
33	625179.0
34	622600.5
35	620022.0
36	633603.0
37	647184.0
38	696487.0
39	786245.5
40	826701.0
41	823960.5
42	821220.0
43	788225.5
44	755231.0
45	711329.5
46	667428.0
47	605594.0
48	543760.0
49	460784.0
50	377808.0
51	293493.0
52	209178.0
53	156316.5
54	103455.0
55	80236.0
56	57017.0
57	45561.0
58	34105.0
59	29223.0
60	24341.0
61	20168.0
62	15995.0
63	11711.5
64	6152.5
65	4877.0
66	4904.0
67	4931.0
68	3798.0
69	2665.0
70	2186.5
71	1708.0
72	1126.5
73	545.0
74	312.0
75	79.0
76	69.0
77	59.0
78	33.0
79	7.0
80	9.5
81	12.0
82	9.0
83	6.0
84	7.0
85	8.0
86	4.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.1727621E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.512414631223436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85686741820103	26.23667737274206
2	10.301200920014745	7.5224343838223735
3	4.726996850108703	5.177822068549683
4	2.84301207605476	4.15220942889947
5	1.977105871836407	3.6094454681158696
6	1.3713109826724035	3.004192511261116
7	1.069385646514021	2.733209648833922
8	0.8247496372897283	2.4090880578938956
9	0.6395429703141841	2.101613229593614
>10	4.103394669578213	26.338279008584504
>50	0.16928594486196602	4.223093910480879
>100	0.10567302994760601	7.487187480436846
>500	0.00735884343361807	1.8252382638875995
>1k	0.0038813262691640604	2.2764319037860417
>5k	1.4028774181355726E-4	0.3519152504657392
>10k+	9.352516120903817E-5	0.5511620126466108
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26233	0.22368560511974253	No Hit
CCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14289	0.12184056766500212	No Hit
AAAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12136	0.10348219813720105	No Hit
GGGAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11903	0.10149543543400662	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.121414223737278	0.0	0.0	0.0	0.0
11	0.1275962106892779	0.0	0.0	0.0	0.0
12	0.18757427444150865	0.0	0.0	0.0	0.0
13	0.313251937456028	0.0	0.0	0.0	0.0
14	0.6884175400961542	0.0	0.0	0.0	0.0
15	1.1733837578823532	0.0	0.0	0.0	0.0
16	1.4208593541691021	0.0	0.0	0.0	0.0
17	1.6162698300021803	0.0	0.0	0.0	0.0
18	1.9161089874920072	0.0	0.0	0.0	0.0
19	2.1031887029773557	0.0	0.0	0.0	0.0
20	2.255768667831268	0.0	0.0	0.0	0.0
21	2.412270996820242	0.0	0.0	0.0	0.0
22	2.550261472467434	0.0	0.0	0.0	0.0
23	2.6994818471708797	0.0	0.0	0.0	0.0
24	2.8831763918700988	0.0	0.0	0.0	3.4107514217930474E-5
25	3.043481708694372	0.0	0.0	0.0	3.4107514217930474E-5
26	3.22900100540425	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
27	3.600022545066898	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
28	3.966328720888917	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
29	4.288116063777982	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
30	4.653671874287206	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
31	5.096412989471607	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
32	5.570430695193851	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
33	5.933556345315047	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
34	6.241777424423931	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
35	6.525108545032279	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
36	6.851363972283893	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
37	7.208350269845862	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
38	7.598403802442115	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
39	8.028797997479625	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
40	8.517592783736786	0.0	0.0	1.7053757108965237E-5	3.4107514217930474E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCAAAT	23315	0.0	42.15269	1
ACGACGT	45	1.5905243E-8	40.88889	16
GCAAATA	39470	0.0	40.341778	2
CGTTAAG	40	2.913639E-7	40.25	20
CCAAATA	23655	0.0	39.884167	2
ACCTAAT	2535	0.0	39.648914	1
GGCCAAT	6100	0.0	39.62787	1
GCCAAAT	9955	0.0	39.623306	1
CCGTTCG	105	0.0	39.428574	32
GGCTAAT	9420	0.0	39.11465	1
AGCGAAT	3705	0.0	39.047234	1
GCCGAAT	3730	0.0	38.970512	1
GCCTAAT	3760	0.0	38.843086	1
TAAAGGA	41055	0.0	38.81793	7
TGGGAAT	10595	0.0	38.814537	1
CCTAATA	10000	0.0	38.778	2
TAAAAGG	12515	0.0	38.777466	7
CCGACGC	95	0.0	38.73684	40
CAAATAA	107970	0.0	38.72742	3
TGCGAAT	7580	0.0	38.62665	1
>>END_MODULE