FastQCFastQC Report
Fri 17 Jun 2016
SRR1527956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11664285
Sequences flagged as poor quality0
Sequence length52
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250350.21462952937106733No Hit
CCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131030.11233436082880346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACT453.110472E-1046.025
TGCGACA700.042.71428334
GGCAAAT221000.041.6185531
CGCCCGA1250.040.47999637
ACTCGTG402.913639E-740.2534
GCAAATA376950.040.0509342
CCAAATA230350.039.6296922
GCCAAAT98500.039.2284241
GGCTAAT92850.038.9154551
ATGCAAT65250.038.8796961
TTGCAAT127550.038.841241
TGGGAAT104200.038.715931
CCGAATA109600.038.655112
GGCCAAT58800.038.6071431
TAAAGGA408600.038.5359767
GGCGAAT67300.038.5156021
CCCAATA48550.038.514932
GTCAAAT93200.038.4978521
CAAATAA1053100.038.4913143
TGGCAAT128650.038.4376221
Produced by FastQC (version 0.11.5)