##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527956_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11664285
Sequences flagged as poor quality	0
Sequence length	52
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.646234038348684	31.0	28.0	34.0	10.0	34.0
2	30.816247545391768	31.0	30.0	34.0	26.0	34.0
3	31.636382941603365	31.0	31.0	34.0	28.0	34.0
4	35.447504669167465	37.0	35.0	37.0	33.0	37.0
5	35.55264501853307	37.0	35.0	37.0	33.0	37.0
6	35.95748354914168	37.0	35.0	37.0	35.0	37.0
7	36.16720184734855	37.0	35.0	37.0	35.0	37.0
8	35.994841947020326	37.0	35.0	37.0	35.0	37.0
9	37.65196237917712	39.0	37.0	39.0	35.0	39.0
10	37.542391839705566	39.0	37.0	39.0	35.0	39.0
11	38.16691550317915	39.0	39.0	39.0	37.0	39.0
12	38.145985801958716	39.0	39.0	39.0	37.0	39.0
13	38.00474199661617	39.0	38.0	39.0	35.0	39.0
14	38.45170938467296	40.0	38.0	40.0	35.0	40.0
15	37.36675509900521	40.0	36.0	40.0	32.0	40.0
16	37.36190482314175	39.0	36.0	40.0	32.0	40.0
17	37.49860535815097	39.0	37.0	40.0	33.0	40.0
18	37.11962362030763	39.0	36.0	40.0	32.0	40.0
19	37.328640203835896	39.0	37.0	40.0	32.0	40.0
20	37.24549160107113	39.0	37.0	40.0	32.0	40.0
21	37.42069188124261	39.0	37.0	40.0	32.0	40.0
22	37.36740700351543	39.0	37.0	40.0	32.0	40.0
23	37.31993019717883	39.0	37.0	40.0	32.0	40.0
24	37.74190325425005	40.0	37.0	40.0	33.0	40.0
25	37.898408775162814	40.0	38.0	40.0	33.0	40.0
26	37.871597273214775	40.0	38.0	40.0	33.0	40.0
27	37.813923185175945	40.0	38.0	40.0	33.0	40.0
28	37.35640804387067	40.0	37.0	40.0	33.0	40.0
29	37.367992380158746	40.0	37.0	40.0	33.0	40.0
30	37.316764293739396	40.0	36.0	40.0	33.0	40.0
31	37.162021246908836	40.0	36.0	40.0	32.0	40.0
32	37.04492337078526	40.0	36.0	40.0	31.0	40.0
33	36.93678883875008	40.0	36.0	40.0	31.0	40.0
34	36.82908699504513	39.0	35.0	40.0	31.0	40.0
35	36.64950213407852	39.0	35.0	40.0	31.0	40.0
36	35.98250882930244	39.0	35.0	40.0	30.0	40.0
37	36.20291479503459	39.0	35.0	40.0	30.0	40.0
38	36.210527949205634	39.0	35.0	40.0	30.0	40.0
39	36.06920295586056	39.0	35.0	40.0	30.0	40.0
40	35.77793555284357	38.0	35.0	40.0	30.0	40.0
41	35.41615023981324	38.0	35.0	40.0	28.0	40.0
42	34.85917250821632	37.0	35.0	40.0	26.0	40.0
43	34.487711762872735	37.0	34.0	40.0	24.0	40.0
44	34.17645342170566	36.0	33.0	40.0	23.0	40.0
45	33.51970489404194	35.0	33.0	40.0	20.0	40.0
46	33.253380897328896	35.0	33.0	40.0	20.0	40.0
47	32.89086660691161	35.0	33.0	40.0	19.0	40.0
48	32.39691219821875	35.0	32.0	39.0	15.0	40.0
49	31.924772842913217	35.0	31.0	39.0	13.0	40.0
50	31.383811266614284	35.0	30.0	38.0	10.0	40.0
51	30.96015392285082	35.0	28.0	38.0	10.0	40.0
52	30.206452002844582	35.0	27.0	37.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	7.0
12	14.0
13	25.0
14	53.0
15	131.0
16	353.0
17	957.0
18	2486.0
19	5348.0
20	10446.0
21	17871.0
22	28924.0
23	43481.0
24	62754.0
25	88508.0
26	121736.0
27	156341.0
28	196198.0
29	246581.0
30	305418.0
31	369690.0
32	442391.0
33	549043.0
34	719137.0
35	955660.0
36	1428433.0
37	2600222.0
38	2769930.0
39	542141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.715388470017665	19.785619092811945	39.5826919524	6.916300484770391
2	28.03654060236011	20.97120397864078	31.200163576250066	19.79209184274904
3	24.954525716749888	23.548884479417296	30.24655176035222	21.25003804348059
4	20.67631235004975	28.552080131786905	28.4039613229615	22.36764619520185
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	100.0	0.0	0.0
11	98.51816892334163	1.3717943277277604	0.06932272316734372	0.04071402576325939
12	98.33368269036636	1.3540649941252294	0.1682486324708287	0.1440036830375801
13	95.46081907292218	3.534918771274879	0.543025140417951	0.4612370153849979
14	33.16757092269265	53.77425191514096	6.097321867564108	6.9608552946022835
15	21.732442237136695	49.1286778400905	17.815914134471168	11.32296578830164
16	47.475477493905544	29.201969945007345	11.723633295997141	11.598919265089974
17	31.328641232617343	24.82435914417386	16.59819697478242	27.248802648426373
18	25.021190754512602	39.54551864945001	24.109493209399464	11.32379738663793
19	34.96576944064724	33.58150971105387	24.471521400583065	6.981199447715826
20	25.84875969680096	28.963224063883896	36.69863176354144	8.489384475773697
21	31.113222970803612	29.738419457343507	20.315758745606782	18.832598826246098
22	23.792782840954246	41.7520062309863	21.869458779513703	12.585752148545753
23	10.282396220599892	46.093489656674194	27.612896975682606	16.011217147043304
24	11.66102337177118	48.54318974544947	17.33758220070926	22.458204682070097
25	20.309911837716584	36.429399658873216	20.3268695852339	22.933818918176296
26	15.046743113701355	43.35573076275143	8.427923357496837	33.16960276605038
27	17.814876779845484	55.60630591587912	7.210514832242182	19.368302472033218
28	21.22509009339192	49.597725021293634	9.162833384129417	20.014351501185025
29	24.883119711152464	41.759919274949134	8.380299349681527	24.97666166421688
30	29.23226755862018	43.28391324457521	9.939340473933893	17.544478722870714
31	19.653772177205887	49.32626389015701	12.100578818161594	18.919385114475514
32	20.877147634852886	58.39497234506873	12.068506556552759	8.659373463525625
33	24.19328745825398	56.17066969814266	11.8017692468934	7.834273596709957
34	19.877017751195208	59.02822161838467	12.61373500390294	8.481025626517184
35	21.272105405517785	53.742788349221584	16.14456436892617	8.84054187633447
36	16.270752986573974	56.11267214407055	18.00467838363003	9.611896485725444
37	16.59036966260684	59.68258663089937	13.80521823669432	9.921825469799478
38	15.70569477683373	59.90046539500706	13.79173262656048	10.602107201598725
39	15.346058502514298	61.843173413543994	12.342822556204688	10.46794552773702
40	14.510722260301426	65.06918340901306	11.384932724123253	9.035161606562255
41	14.664207879008442	66.11209345450663	11.000785731830112	8.222912934654802
42	16.34358213983969	63.04986546539286	11.87897072130868	8.727581673458767
43	17.31251422611845	60.707098634849885	12.89800446405416	9.082382674977506
44	18.382146869696687	61.439873939980025	10.945437289983913	9.232541900339369
45	18.73236979377647	59.92210409810803	11.475448345097877	9.870077763017623
46	19.7278873072803	58.852394295921265	11.790838444019501	9.628879952778933
47	22.29303382076141	56.46987363563218	11.557613689994714	9.679478853611688
48	21.631956009305327	56.62528821955225	11.545071129520583	10.19768464162184
49	19.634739720437214	57.72244934001527	12.039794981003979	10.603015958543537
50	17.93217501115585	58.6528364147481	12.65628368991327	10.758704884182785
51	17.494891457127462	57.50287308652009	13.23930270908161	11.762932747270835
52	18.24067227438287	56.53879341939947	12.757352893897911	12.463181412319743
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	180.0
1	942.5
2	1705.0
3	4884.5
4	8064.0
5	18903.0
6	29742.0
7	37560.0
8	45378.0
9	74153.0
10	102928.0
11	106632.5
12	110337.0
13	85729.0
14	59002.0
15	56883.0
16	70817.0
17	84751.0
18	136297.5
19	187844.0
20	319422.0
21	451000.0
22	592222.5
23	733445.0
24	765128.5
25	796812.0
26	740561.0
27	684310.0
28	648184.0
29	612058.0
30	613222.0
31	614386.0
32	619876.5
33	625367.0
34	622637.5
35	619908.0
36	634727.0
37	649546.0
38	694621.5
39	780828.5
40	821960.0
41	820123.5
42	818287.0
43	786690.5
44	755094.0
45	710364.0
46	665634.0
47	604666.5
48	543699.0
49	460121.0
50	376543.0
51	293584.0
52	210625.0
53	157282.5
54	103940.0
55	80533.5
56	57127.0
57	45363.0
58	33599.0
59	28798.5
60	23998.0
61	19978.5
62	15959.0
63	11696.0
64	6201.0
65	4969.0
66	4935.0
67	4901.0
68	3760.5
69	2620.0
70	2162.0
71	1704.0
72	1132.5
73	561.0
74	321.5
75	82.0
76	70.5
77	59.0
78	32.0
79	5.0
80	6.5
81	8.0
82	6.0
83	4.0
84	8.0
85	12.0
86	6.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.1664285E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.86296877238598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60151324225787	26.394443467049328
2	10.398765843761405	7.666587611398613
3	4.766763697538112	5.271511839830717
4	2.940900721507909	4.336413258385338
5	1.9777051840784161	3.6452042220834264
6	1.4098293757579472	3.1182297751774573
7	1.0478736418381656	2.7039413335540674
8	0.8118228705498782	2.3940960900631127
9	0.6755376274308508	2.2412090218099654
>10	4.093232823974011	26.185608510533743
>50	0.16239784937763488	4.101766504755017
>100	0.10267069463328862	7.222941301314129
>500	0.00716613368678688	1.7765206250193732
>1k	0.0035859216704066067	2.075617977983892
>5k	1.406231624196956E-4	0.3388036946846153
>10k+	9.374877494646373E-5	0.5271047663574749
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25035	0.21462952937106733	No Hit
CCAAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13103	0.11233436082880346	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.12299939516224098	0.0	0.0	0.0	0.0
11	0.12954930370785694	0.0	0.0	0.0	0.0
12	0.189132895844023	0.0	0.0	0.0	0.0
13	0.3159387823599989	0.0	0.0	0.0	0.0
14	0.7007887753085594	0.0	0.0	0.0	0.0
15	1.1869737407822254	0.0	0.0	0.0	0.0
16	1.4378163770861223	0.0	0.0	0.0	0.0
17	1.6327190222118202	0.0	0.0	0.0	0.0
18	1.9384128559958882	0.0	0.0	0.0	0.0
19	2.128823155469881	0.0	0.0	0.0	0.0
20	2.280688443397945	0.0	0.0	0.0	0.0
21	2.438366346501307	0.0	0.0	0.0	0.0
22	2.574028326639824	0.0	0.0	0.0	0.0
23	2.71870929079665	0.0	0.0	0.0	0.0
24	2.8988917880521607	0.0	0.0	0.0	8.573178724628214E-6
25	3.061396390777489	0.0	0.0	0.0	8.573178724628214E-6
26	3.2443737442972287	0.0	0.0	0.0	8.573178724628214E-6
27	3.6161324933332817	0.0	0.0	0.0	8.573178724628214E-6
28	3.9702647869114993	0.0	0.0	0.0	8.573178724628214E-6
29	4.2884840348122495	0.0	0.0	0.0	8.573178724628214E-6
30	4.649654908123387	0.0	0.0	0.0	8.573178724628214E-6
31	5.086638400896412	0.0	0.0	0.0	8.573178724628214E-6
32	5.55397094635462	0.0	0.0	0.0	8.573178724628214E-6
33	5.913710098818744	0.0	0.0	0.0	8.573178724628214E-6
34	6.208198788009724	0.0	0.0	0.0	8.573178724628214E-6
35	6.491405173999092	0.0	0.0	0.0	8.573178724628214E-6
36	6.807018175567555	0.0	0.0	0.0	8.573178724628214E-6
37	7.154900621855519	0.0	0.0	0.0	8.573178724628214E-6
38	7.528956982789772	0.0	0.0	0.0	8.573178724628214E-6
39	7.959570603770398	0.0	0.0	0.0	8.573178724628214E-6
40	8.441494699417923	0.0	0.0	0.0	8.573178724628214E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTACT	45	3.110472E-10	46.0	25
TGCGACA	70	0.0	42.714283	34
GGCAAAT	22100	0.0	41.618553	1
CGCCCGA	125	0.0	40.479996	37
ACTCGTG	40	2.913639E-7	40.25	34
GCAAATA	37695	0.0	40.050934	2
CCAAATA	23035	0.0	39.629692	2
GCCAAAT	9850	0.0	39.228424	1
GGCTAAT	9285	0.0	38.915455	1
ATGCAAT	6525	0.0	38.879696	1
TTGCAAT	12755	0.0	38.84124	1
TGGGAAT	10420	0.0	38.71593	1
CCGAATA	10960	0.0	38.65511	2
GGCCAAT	5880	0.0	38.607143	1
TAAAGGA	40860	0.0	38.535976	7
GGCGAAT	6730	0.0	38.515602	1
CCCAATA	4855	0.0	38.51493	2
GTCAAAT	9320	0.0	38.497852	1
CAAATAA	105310	0.0	38.491314	3
TGGCAAT	12865	0.0	38.437622	1
>>END_MODULE