FastQCFastQC Report
Fri 17 Jun 2016
SRR1527955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12430706
Sequences flagged as poor quality0
Sequence length59
%GC34

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175770.14139985291261814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAAAT247500.047.592931
CCTAACT3100.046.1612946
GCAAATA422700.045.6963542
CCAAATA249150.045.012242
GCCAAAT107150.044.7890781
GGCCAAT69850.044.4259151
ACCTAAT28050.044.402851
TAGCAAT85050.044.1822431
CGGGTTA304.3472595E-544.16666844
CCGCGAC1200.044.16666815
TAAAGGA442400.044.068837
ACCGAAT27650.043.9909551
TTGCAAT136750.043.9308931
GTCAAAT100350.043.9157941
TACGAAT54350.043.8822441
CCGAATA114850.043.770572
GCGAATA194400.043.7440832
CAAATAA1159650.043.7336273
TGGCAAT136650.043.6721571
TTGGAAT146750.043.664051
Produced by FastQC (version 0.11.5)