##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527955_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12430706
Sequences flagged as poor quality	0
Sequence length	59
%GC	34
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.664439815405498	31.0	30.0	33.0	16.0	34.0
2	30.870788191756766	31.0	30.0	34.0	27.0	34.0
3	31.501655336390385	31.0	31.0	34.0	28.0	34.0
4	35.33100871342303	35.0	35.0	37.0	33.0	37.0
5	35.19583095280348	37.0	35.0	37.0	33.0	37.0
6	35.69249791604757	37.0	35.0	37.0	33.0	37.0
7	35.92532934171237	37.0	35.0	37.0	35.0	37.0
8	35.79373110425104	37.0	35.0	37.0	35.0	37.0
9	37.40782277370248	39.0	37.0	39.0	34.0	39.0
10	37.264023781111064	39.0	37.0	39.0	33.0	39.0
11	37.96730330521854	39.0	37.0	39.0	35.0	39.0
12	37.9731093310388	39.0	38.0	39.0	35.0	39.0
13	37.77107905214716	39.0	37.0	39.0	35.0	39.0
14	37.938973216806836	40.0	38.0	40.0	33.0	40.0
15	36.90971470164285	39.0	36.0	40.0	31.0	40.0
16	36.927135031590325	39.0	36.0	40.0	31.0	40.0
17	36.95937318443538	39.0	36.0	40.0	32.0	40.0
18	36.43078245113351	38.0	35.0	40.0	31.0	40.0
19	36.65560379273711	39.0	36.0	40.0	31.0	40.0
20	36.684920631217565	39.0	36.0	40.0	31.0	40.0
21	36.81141682539994	39.0	36.0	40.0	31.0	40.0
22	36.77781720523355	39.0	36.0	40.0	31.0	40.0
23	36.76829892043139	39.0	36.0	40.0	31.0	40.0
24	37.17952174236926	39.0	36.0	40.0	32.0	40.0
25	37.327749284714805	39.0	37.0	40.0	32.0	40.0
26	37.210642340024776	40.0	37.0	40.0	32.0	40.0
27	37.03056230273646	39.0	36.0	40.0	31.0	40.0
28	36.761473563931126	39.0	36.0	40.0	31.0	40.0
29	36.62450137586715	39.0	35.0	40.0	30.0	40.0
30	36.50266002590681	39.0	35.0	40.0	30.0	40.0
31	36.37345996277283	39.0	35.0	40.0	30.0	40.0
32	36.167507300068074	38.0	35.0	40.0	30.0	40.0
33	35.98275817962391	38.0	35.0	40.0	30.0	40.0
34	35.85950411826971	38.0	35.0	40.0	29.0	40.0
35	35.72421180261202	38.0	35.0	40.0	29.0	40.0
36	35.32116743811655	38.0	34.0	40.0	27.0	40.0
37	35.35705172336954	38.0	35.0	40.0	27.0	40.0
38	35.27486934370421	38.0	35.0	40.0	27.0	40.0
39	35.09877411628913	38.0	34.0	40.0	26.0	40.0
40	34.75274734998961	37.0	33.0	40.0	25.0	40.0
41	34.36099775829305	37.0	33.0	40.0	24.0	40.0
42	34.027559496620704	36.0	33.0	40.0	23.0	40.0
43	33.54088174879207	35.0	33.0	40.0	22.0	40.0
44	33.2252464984692	35.0	33.0	39.0	21.0	40.0
45	32.794312969834536	35.0	32.0	39.0	20.0	40.0
46	32.2867369721398	35.0	31.0	39.0	17.0	40.0
47	31.863647487117788	35.0	31.0	38.0	15.0	40.0
48	31.32439090748345	35.0	29.0	38.0	13.0	40.0
49	30.903241537528118	35.0	28.0	38.0	11.0	40.0
50	30.38110023678462	35.0	27.0	37.0	10.0	40.0
51	29.879419077243078	34.0	26.0	37.0	9.0	40.0
52	29.349440249009188	34.0	23.0	37.0	9.0	40.0
53	28.70861260816562	33.0	22.0	36.0	8.0	40.0
54	27.962552649865582	33.0	20.0	36.0	8.0	39.0
55	27.227530761326026	33.0	18.0	35.0	8.0	39.0
56	26.403358023269153	33.0	15.0	35.0	8.0	39.0
57	25.617508530891165	31.0	10.0	35.0	8.0	39.0
58	24.849236640300237	31.0	10.0	35.0	8.0	38.0
59	24.02267200270041	29.0	9.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	8.0
13	33.0
14	98.0
15	439.0
16	1351.0
17	4238.0
18	10456.0
19	20689.0
20	36817.0
21	58668.0
22	86792.0
23	122928.0
24	165586.0
25	210942.0
26	260913.0
27	319873.0
28	376713.0
29	434290.0
30	508478.0
31	613146.0
32	748413.0
33	909056.0
34	1083859.0
35	1225016.0
36	1465472.0
37	1936847.0
38	1678836.0
39	150745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.93680133694739	20.21655085399011	39.006014622178334	6.840633186884156
2	27.62674943804479	21.305797112408577	31.122287020544125	19.945166429002505
3	24.893147661926847	23.76120069125599	30.058405371344154	21.28724627547301
4	20.705388736568946	28.661026976263454	28.180981836429886	22.452602450737714
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	100.0	0.0	0.0
10	0.0	100.0	0.0	0.0
11	98.51053512165761	1.3830831490986917	0.06332705479479604	0.04305467444890097
12	98.36359254253138	1.3205525092460557	0.1544240528253182	0.16143089539725258
13	95.49472089517683	3.50919730544669	0.515055218907116	0.4810265804693635
14	32.802988020149456	54.11185816799142	6.055416321486487	7.0297374903726295
15	21.740470734325147	49.46509071970651	17.499730103825158	11.29470844214319
16	47.45839053711028	29.155077756645518	11.702931434465588	11.68360027177861
17	31.137780911237062	24.863728576639172	16.455694471416184	27.542796040707586
18	25.114679729373375	39.78034715003315	23.742867058395557	11.362106062197915
19	35.04065658056751	33.395737941191754	24.610822587228753	6.952782891011982
20	25.817391224601405	28.94427717942971	36.81804557198923	8.420286023979653
21	31.29991973102734	29.610779950873265	20.434237604847223	18.655062713252168
22	23.80664461053137	41.52250081371082	22.031998826132643	12.638855749625161
23	10.087898466909282	46.011119561511634	27.940102517105625	15.960879454473462
24	11.33561520962687	48.886394706784955	17.51673637844866	22.261253705139513
25	19.920445387414038	36.431671700706296	20.60984307729585	23.038039834583813
26	14.89291115082281	43.463082466917	8.436962470192762	33.20704391206743
27	17.64101733240252	55.79400719476432	7.16176538967296	19.4032100831602
28	20.778095789571406	50.1590336059754	8.969627308376532	20.09324329607667
29	24.76883452959148	41.8628676440421	8.245460877282433	25.122836949083986
30	29.105088640983062	43.579431449830764	9.736896681491784	17.578583227694388
31	19.452113178446982	49.81569027535524	11.77328142102307	18.95891512517471
32	20.63946327746791	59.0990970263475	11.784294472091931	8.477145224092663
33	23.997430234453297	56.91304258985773	11.5457883084034	7.543738867285575
34	19.617759441820922	59.81370647813568	12.410614489635584	8.157919590407818
35	21.049423902391386	54.45234566725333	15.97889934811426	8.519331082241024
36	15.626900032870218	57.69444631704748	17.885299515570555	8.793354134511748
37	16.31978103254956	60.46551981842383	13.574056051200953	9.64064309782566
38	15.432542608601635	60.737950040810226	13.459726261726408	10.369781088861727
39	15.126349219424867	62.715013934043654	12.017298132543718	10.141338713987766
40	14.20090701203938	66.06121969259027	11.024989248398281	8.712884046972071
41	14.275303430070666	67.28906628473072	10.554501087870632	7.881129197327971
42	15.608035456715008	64.62142214609531	11.385419299595695	8.385123097593974
43	16.89648198581802	61.882518981625026	12.491301781250396	8.729697251306563
44	17.781854063638864	62.74642003438904	10.494126399578592	8.97759950239351
45	18.055844937528086	61.723734758106254	10.772694648236392	9.447725656129267
46	19.280280621229398	59.974791455931786	11.301329144137107	9.443598778701709
47	21.98990145853341	57.45994636185587	11.092057040042617	9.458095139568098
48	21.16292509854227	57.64041076991122	11.140083274433488	10.056580857113023
49	19.243935139323543	58.71685003249212	11.637054242936804	10.40216058524753
50	17.3314773915496	59.82573314822183	12.200867754413949	10.641921705814617
51	17.138085318726066	58.319543556094075	12.903064395537953	11.639306729641904
52	17.69868903664844	57.242484859669275	12.476041183823348	12.582784919858936
53	17.038943725320188	56.950634984046765	13.152720368416727	12.857700922216326
54	17.048991424944006	56.62600338226969	13.441030622074079	12.883974570712233
55	18.194662475325217	54.5412062677695	13.880820606649372	13.383310650255906
56	19.065176185487775	52.94517463448979	14.051366028606903	13.938283151415535
57	19.625393762832136	51.0655388358473	13.964508532339192	15.344558868981375
58	20.29024739222374	50.06330292100867	14.162912388081578	15.483537298686013
59	20.792849577489804	49.11299486931796	14.415005873359082	15.679149679833149
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	152.0
1	739.0
2	1326.0
3	3790.0
4	14298.5
5	22343.0
6	27887.5
7	33432.0
8	54681.0
9	78108.0
10	80286.0
11	62747.0
12	45208.0
13	42447.0
14	41537.0
15	43388.0
16	55553.0
17	67718.0
18	116298.0
19	267358.5
20	369839.0
21	473978.0
22	578117.0
23	641658.5
24	705200.0
25	745069.5
26	797014.0
27	809089.0
28	790715.0
29	772341.0
30	719444.5
31	603817.0
32	541086.0
33	509588.5
34	478091.0
35	491175.0
36	549784.0
37	595309.0
38	655860.5
39	716412.0
40	758293.5
41	800175.0
42	790954.5
43	736129.0
44	690524.0
45	638057.5
46	585591.0
47	536403.5
48	430955.5
49	374695.0
50	307416.5
51	240138.0
52	188931.5
53	106954.0
54	76183.0
55	61096.5
56	46010.0
57	42032.0
58	31387.5
59	24721.0
60	20568.0
61	16415.0
62	13164.0
63	9913.0
64	8394.5
65	5900.5
66	4925.0
67	3785.5
68	2646.0
69	2108.0
70	1393.5
71	1217.0
72	1613.5
73	2010.0
74	1195.5
75	244.0
76	107.0
77	62.0
78	17.0
79	15.0
80	13.0
81	10.0
82	9.5
83	12.0
84	6.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.2430706E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.907433762289934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.35855369305771	43.0447229051116
2	7.716193338442134	8.164879759013003
3	3.216490493982946	5.105287732723139
4	1.8622228058922465	3.9410171901347892
5	1.1954660525730312	3.1624520495786936
6	0.8390984566912569	2.6636727610459427
7	0.6014317738335496	2.2274148215644543
8	0.4726324784060441	2.000461723613977
9	0.3703970068816957	1.7637079596609386
>10	2.2549285693111525	20.653226448374646
>50	0.0757578236393564	2.717933382532715
>100	0.03524920402803815	3.325981666117883
>500	0.001089816400715588	0.3838018067178278
>1k	3.968983964491115E-4	0.38694673579550853
>5k	6.105897572562749E-5	0.22857216761970003
>10k+	3.0529487862813744E-5	0.22992089039521024
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAAATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17577	0.14139985291261814	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.10843310106441259	0.0	0.0	0.0	0.0
11	0.11398387187340767	0.0	0.0	0.0	0.0
12	0.16800332981891777	0.0	0.0	0.0	0.0
13	0.28098162727040604	0.0	0.0	0.0	0.0
14	0.62307000101201	0.0	0.0	0.0	0.0
15	1.0543166252986758	0.0	0.0	0.0	0.0
16	1.2765646617336135	0.0	0.0	0.0	0.0
17	1.45336877889317	0.0	0.0	0.0	0.0
18	1.7277538379557846	0.0	0.0	0.0	0.0
19	1.8979050747399222	0.0	0.0	0.0	0.0
20	2.034791909646966	0.0	0.0	0.0	0.0
21	2.1785005614323114	0.0	0.0	0.0	0.0
22	2.3010358381897213	0.0	0.0	0.0	0.0
23	2.432323634715518	0.0	0.0	0.0	0.0
24	2.5959989722224948	0.0	0.0	0.0	4.0222976876775945E-5
25	2.7402144335164875	0.0	0.0	0.0	4.0222976876775945E-5
26	2.9102047783931178	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
27	3.240202125285563	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
28	3.57204972911434	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
29	3.8614942707196196	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
30	4.193695836744912	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
31	4.602852002130852	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
32	5.043068350261039	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
33	5.380523037066439	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
34	5.655286192111695	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
35	5.913944067215491	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
36	6.211457337982251	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
37	6.535879780279576	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
38	6.8908234174309975	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
39	7.286609465303097	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
40	7.734870408808639	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
41	8.20271994205317	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
42	8.578257743365501	0.0	0.0	8.044595375355189E-6	4.0222976876775945E-5
43	8.9168225843327	0.0	0.0	8.044595375355189E-6	8.044595375355189E-5
44	9.24488922833506	0.0	0.0	8.044595375355189E-6	8.044595375355189E-5
45	9.575699079360415	0.0	0.0	8.044595375355189E-6	8.044595375355189E-5
46	9.908664881946367	0.0	0.0	8.044595375355189E-6	8.849054912890707E-5
47	10.234479039243627	0.0	0.0	8.044595375355189E-6	8.849054912890707E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCAAAT	24750	0.0	47.59293	1
CCTAACT	310	0.0	46.16129	46
GCAAATA	42270	0.0	45.696354	2
CCAAATA	24915	0.0	45.01224	2
GCCAAAT	10715	0.0	44.789078	1
GGCCAAT	6985	0.0	44.425915	1
ACCTAAT	2805	0.0	44.40285	1
TAGCAAT	8505	0.0	44.182243	1
CGGGTTA	30	4.3472595E-5	44.166668	44
CCGCGAC	120	0.0	44.166668	15
TAAAGGA	44240	0.0	44.06883	7
ACCGAAT	2765	0.0	43.990955	1
TTGCAAT	13675	0.0	43.930893	1
GTCAAAT	10035	0.0	43.915794	1
TACGAAT	5435	0.0	43.882244	1
CCGAATA	11485	0.0	43.77057	2
GCGAATA	19440	0.0	43.744083	2
CAAATAA	115965	0.0	43.733627	3
TGGCAAT	13665	0.0	43.672157	1
TTGGAAT	14675	0.0	43.66405	1
>>END_MODULE