FastQCFastQC Report
Fri 17 Jun 2016
SRR1527954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14340460
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA270150.044.5781942
AAATCGT60250.044.5493771
TTACGTA462050.044.501682
CGACGTA146450.044.4609072
GACGTAT745250.044.4599763
CCGTATT1907050.044.418874
TTGGGGC2244900.044.399669
TTTCGTA829350.044.3970572
CGCGTAT775300.044.374313
TACTCGT83050.044.3660431
ACACGTA176950.044.362252
ACCGTAT543350.044.3491333
TCCGTAT706150.044.2769973
CTTACGT103250.044.262471
GCCGTAT536500.044.2551733
ATACGTA273750.044.2356152
TTCGTAT2254500.044.2228433
TCGTATT5988550.044.2033354
ATTCGTA431300.044.1602132
ACGTATT3730250.044.1385424
Produced by FastQC (version 0.11.5)