##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14340460
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.405707557498154	31.0	31.0	33.0	30.0	34.0
2	32.13128623489065	33.0	31.0	34.0	30.0	34.0
3	32.36875964927206	34.0	31.0	34.0	30.0	34.0
4	35.995136766881956	37.0	35.0	37.0	35.0	37.0
5	36.10803649255324	37.0	35.0	37.0	35.0	37.0
6	36.07899900003208	37.0	35.0	37.0	35.0	37.0
7	34.432698393217514	35.0	35.0	35.0	32.0	37.0
8	35.398015335630795	35.0	35.0	37.0	33.0	37.0
9	37.9757422704711	39.0	38.0	39.0	35.0	39.0
10	38.13908291644759	39.0	39.0	39.0	35.0	39.0
11	37.700747744493555	39.0	37.0	39.0	35.0	39.0
12	38.02754890707829	39.0	38.0	39.0	37.0	39.0
13	38.13006598114705	39.0	39.0	39.0	37.0	39.0
14	38.820320408131956	40.0	39.0	40.0	36.0	40.0
15	38.2189716368931	40.0	38.0	40.0	34.0	40.0
16	37.35096168463215	39.0	36.0	40.0	33.0	40.0
17	37.32629155550101	40.0	36.0	40.0	33.0	40.0
18	37.34915023646382	40.0	36.0	40.0	33.0	40.0
19	37.46566839557448	40.0	36.0	40.0	33.0	40.0
20	37.48428530186619	40.0	36.0	40.0	33.0	40.0
21	37.22001037623619	40.0	36.0	40.0	32.0	40.0
22	37.27146067838828	40.0	36.0	40.0	32.0	40.0
23	37.22428213599843	40.0	36.0	40.0	32.0	40.0
24	37.1682114102337	40.0	35.0	40.0	32.0	40.0
25	37.37685262536906	40.0	36.0	40.0	33.0	40.0
26	37.408435015334234	40.0	36.0	40.0	33.0	40.0
27	37.40394743264861	40.0	36.0	40.0	33.0	40.0
28	37.399837173981865	40.0	36.0	40.0	33.0	40.0
29	37.40845447077709	40.0	35.0	40.0	33.0	40.0
30	37.37093454463804	40.0	35.0	40.0	33.0	40.0
31	37.33168817457739	40.0	35.0	40.0	33.0	40.0
32	37.2493705222845	40.0	35.0	40.0	33.0	40.0
33	37.165397414029954	40.0	35.0	40.0	33.0	40.0
34	37.06371573854674	40.0	35.0	40.0	33.0	40.0
35	36.95701490747159	39.0	35.0	40.0	32.0	40.0
36	36.87066168030872	39.0	35.0	40.0	32.0	40.0
37	36.70644944443902	39.0	35.0	40.0	32.0	40.0
38	36.44178289957226	39.0	35.0	40.0	31.0	40.0
39	36.348449212926226	38.0	35.0	40.0	31.0	40.0
40	36.23233968784823	38.0	35.0	40.0	31.0	40.0
41	36.094667325873786	38.0	35.0	40.0	31.0	40.0
42	35.93356161517831	38.0	35.0	40.0	31.0	40.0
43	35.72887599142566	37.0	35.0	40.0	30.0	40.0
44	35.54912666678754	37.0	35.0	40.0	30.0	40.0
45	35.38208460537528	36.0	34.0	40.0	30.0	40.0
46	35.22967861560926	36.0	34.0	40.0	30.0	40.0
47	35.055820664051225	36.0	34.0	39.0	29.0	40.0
48	34.87961836649591	35.0	34.0	39.0	29.0	40.0
49	34.7092176959456	35.0	34.0	39.0	29.0	40.0
50	34.54499088592695	35.0	34.0	39.0	29.0	40.0
51	34.384325328476216	35.0	34.0	39.0	28.0	40.0
52	33.737711272860146	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	3.0
13	12.0
14	14.0
15	41.0
16	165.0
17	544.0
18	1409.0
19	3226.0
20	6468.0
21	11646.0
22	19267.0
23	29794.0
24	44352.0
25	64466.0
26	88585.0
27	120489.0
28	159316.0
29	207453.0
30	266850.0
31	334998.0
32	418140.0
33	539829.0
34	845905.0
35	1581213.0
36	1396345.0
37	2667304.0
38	4326588.0
39	1206036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.868913549495623	19.24206754873972	33.586328472029486	18.30269042973517
2	28.9987559673818	19.607920526956597	33.92540406653622	17.467919439125385
3	22.572985803802666	21.863071338018443	35.031979448358	20.531963409820882
4	22.056544908601257	25.05757137497681	40.14553229115384	12.740351425268088
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.57866762990867	0.30630816584684173	0.10493387241413456	0.010090331830359695
12	99.74318118107787	0.1948473061533591	0.04439188143197638	0.01757963133679115
13	99.25286218154787	0.3855106461020079	0.10671205805113644	0.25491511429898345
14	67.42448986992049	20.414540398285688	4.46321805576669	7.697751676027129
15	38.29969889389881	23.854088362576935	16.046416921075057	21.799795822449212
16	31.172730860795262	16.618504566799114	26.075976642311332	26.132787930094292
17	25.384039284653355	15.219679145578317	28.495773496805544	30.90050807296279
18	24.07551082740721	16.219103152897464	31.150960289976755	28.554425729718574
19	27.366848762173596	15.264607969339897	28.50103832094647	28.867504947540034
20	32.51239499988146	15.493972996682114	24.308195134605164	27.685436868831264
21	33.540723240398144	17.525909210722666	21.430051755661953	27.503315793217233
22	32.84819315419449	18.230307814393683	21.467846917044504	27.453652114367323
23	32.219461579335665	19.523620581208693	20.515150838954956	27.741767000500683
24	31.550849833268945	21.593379849739826	20.194986771693515	26.66078354529771
25	33.02525163070083	19.422201240406515	21.991449367732972	25.561097761159683
26	31.752496084504962	18.54558361447262	22.14640255612442	27.555517744898
27	32.784792119639114	19.610577345496587	20.74807921084819	26.856551324016102
28	34.00782820076901	17.55915082221909	20.13005858947342	28.30296238753848
29	31.50090722333872	17.327665918666487	20.099703914658246	31.071722943336542
30	29.77443540862706	16.867617914627566	21.90737256684932	31.450574109896056
31	29.10910807603103	17.12810467725582	21.19249312783551	32.570294118877634
32	31.177096132202177	20.589116388177228	19.748264700016595	28.485522779604
33	32.15440787812943	20.36552523419751	19.416894576603543	28.06317231106952
34	31.994622208771546	19.428365617281454	21.072671309009614	27.504340864937387
35	34.99336144028853	19.423554056146035	19.47496802752492	26.108116476040514
36	37.659642717179224	18.658996991728298	18.88469407536439	24.796666215728084
37	36.652889795724825	18.68585108148553	20.77493330060542	23.886325822184226
38	31.8953645838418	21.636042358473855	23.943165003075215	22.525428054609126
39	32.44681132962262	23.47096257721161	22.27892271238161	21.803303380784158
40	31.674792858806484	24.89138423732572	19.03654415548734	24.397278748380458
41	33.54616936974128	23.686757607496553	18.048305284488784	24.71876773827339
42	35.18366216983277	22.947778523143608	17.587462326870966	24.281096980152657
43	34.21482992874705	21.72459600319655	17.868359871301198	26.19221419675519
44	31.861369858428528	21.842144533717885	18.480829764177717	27.815655843675863
45	29.121513535827997	21.588749593806615	19.856587585056545	29.43314928530884
46	28.458849995048972	19.423784174287295	21.364307700031937	30.753058130631793
47	29.23950138280083	17.155628201605808	23.24670198863914	30.358168426954222
48	30.394924570062603	17.112840173885637	22.224593911213447	30.26764134483831
49	31.600129981883423	18.65195398195037	21.64474500817965	28.10317102798655
50	29.94715650683451	21.288884735915026	21.0174987413235	27.746460015926967
51	29.488405532319046	20.554996143777814	21.963695725241728	27.992902598661413
52	30.024441335912517	19.533766699255114	23.90347311034653	26.538318854485837
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	7.0
6	11.0
7	11.5
8	12.0
9	311.5
10	611.0
11	1088.5
12	1566.0
13	2340.5
14	3585.0
15	4055.0
16	4715.0
17	5375.0
18	6742.0
19	8109.0
20	10313.5
21	12518.0
22	16113.0
23	19708.0
24	25396.5
25	31085.0
26	36309.5
27	41534.0
28	44759.5
29	47985.0
30	49993.5
31	52002.0
32	55688.0
33	59374.0
34	62852.0
35	66330.0
36	77177.0
37	88024.0
38	93047.0
39	110455.5
40	122841.0
41	152476.5
42	182112.0
43	215221.5
44	248331.0
45	299025.5
46	349720.0
47	408295.5
48	466871.0
49	541837.0
50	616803.0
51	713369.5
52	809936.0
53	951874.0
54	1093812.0
55	1223295.0
56	1352778.0
57	1487678.0
58	1622578.0
59	1666737.5
60	1710897.0
61	1598395.0
62	1485893.0
63	1305850.5
64	977093.5
65	828379.0
66	727889.5
67	627400.0
68	539008.0
69	450616.0
70	397022.5
71	343429.0
72	269652.0
73	195875.0
74	150590.5
75	105306.0
76	73055.0
77	40804.0
78	28168.0
79	15532.0
80	9549.5
81	3567.0
82	2192.5
83	818.0
84	756.5
85	695.0
86	419.0
87	143.0
88	84.5
89	14.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.434046E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.071698664299742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.82715886118008	4.300411658535728
2	9.018039115172618	1.0950963210039282
3	4.322207857594487	0.7872943102734687
4	2.796860490833781	0.6792677642571275
5	1.7066146296488922	0.5181024883656788
6	1.176584820004822	0.4286321088035177
7	0.7646835244734597	0.3250049553910267
8	0.5259781098686128	0.2554864469712127
9	0.3700937659653572	0.20223880420297685
>10	2.536070025762338	3.2350397280912686
>50	0.9864476179864767	4.502207175804564
>100	4.529834965031677	64.93496110739007
>500	0.40377817261302096	15.876576815222535
>1k	0.035531546988990866	2.817487847203755
>5k	1.1649687537374054E-4	0.042192468483199394
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	6.066750996829948E-4	0.0	0.0	0.0	0.0
12	0.003005482390383572	0.0	0.0	0.0	0.0
13	0.00391898167841199	0.0	0.0	0.0	0.0
14	0.018346691807654705	0.0	0.0	0.0	0.0
15	0.03759293634932213	0.0	0.0	0.0	0.0
16	0.0487153131768437	0.0	0.0	0.0	0.0
17	0.05717389818736637	0.0	0.0	0.0	0.0
18	0.06513738053033166	0.0	0.0	0.0	0.0
19	0.07337282067660313	0.0	0.0	0.0	0.0
20	0.08327487402775086	0.0	0.0	0.0	0.0
21	0.09383938869464438	0.0	0.0	0.0	0.0
22	0.10500360518421306	0.0	0.0	0.0	0.0
23	0.11908962474007109	0.0	0.0	0.0	0.0
24	0.13269448818238747	0.0	0.0	0.0	0.0
25	0.14708733192659093	0.0	0.0	0.0	0.0
26	0.16234486201976783	0.0	0.0	0.0	0.0
27	0.1792899251488446	0.0	0.0	0.0	0.0
28	0.19565620628626976	0.0	0.0	0.0	0.0
29	0.21419814985014426	0.0	0.0	0.0	0.0
30	0.23441367989590292	0.0	0.0	0.0	0.0
31	0.25179805947647427	0.0	0.0	0.0	0.0
32	0.2681922337219308	0.0	0.0	0.0	0.0
33	0.2822433868927496	0.0	0.0	0.0	0.0
34	0.2957854908419953	0.0	0.0	0.0	0.0
35	0.3088813050627386	0.0	0.0	0.0	0.0
36	0.32190738651340334	0.0	0.0	0.0	0.0
37	0.33376893070375707	0.0	0.0	0.0	0.0
38	0.34823848049504685	0.0	0.0	0.0	0.0
39	0.3679310147652167	0.0	0.0	0.0	0.0
40	0.39896907072715937	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGTA	27015	0.0	44.578194	2
AAATCGT	6025	0.0	44.549377	1
TTACGTA	46205	0.0	44.50168	2
CGACGTA	14645	0.0	44.460907	2
GACGTAT	74525	0.0	44.459976	3
CCGTATT	190705	0.0	44.41887	4
TTGGGGC	224490	0.0	44.39966	9
TTTCGTA	82935	0.0	44.397057	2
CGCGTAT	77530	0.0	44.37431	3
TACTCGT	8305	0.0	44.366043	1
ACACGTA	17695	0.0	44.36225	2
ACCGTAT	54335	0.0	44.349133	3
TCCGTAT	70615	0.0	44.276997	3
CTTACGT	10325	0.0	44.26247	1
GCCGTAT	53650	0.0	44.255173	3
ATACGTA	27375	0.0	44.235615	2
TTCGTAT	225450	0.0	44.222843	3
TCGTATT	598855	0.0	44.203335	4
ATTCGTA	43130	0.0	44.160213	2
ACGTATT	373025	0.0	44.138542	4
>>END_MODULE