FastQCFastQC Report
Fri 17 Jun 2016
SRR1527953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14223025
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA180150.044.5445442
ACCGTAT527900.044.527373
TTACGTA449550.044.480482
ATTACGT87300.044.4192471
CCGTATT1873500.044.3745964
TTGGGGC2226500.044.3492479
TACTCGT83050.044.338351
GACGTAT743400.044.3262023
ATCGTAT1244350.044.31433
CGCGTAT781850.044.293793
TCGTATT5946050.044.279084
TTCGTAT2254400.044.2717363
AAATCGT62400.044.2676281
TTTCGTA829600.044.2616922
GCGCGTA278250.044.2558862
CGACGTA143900.044.2418332
AACGTAT746400.044.240493
ATTCGTA437850.044.229762
TCCGTAT697850.044.2268373
AAACGTA167100.044.2244152
Produced by FastQC (version 0.11.5)