##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527953_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14223025
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.362462767238334	31.0	31.0	33.0	30.0	34.0
2	31.97356736699823	33.0	31.0	34.0	30.0	34.0
3	32.299710364004845	34.0	31.0	34.0	30.0	34.0
4	35.967051523849534	37.0	35.0	37.0	35.0	37.0
5	36.09144911156382	37.0	35.0	37.0	35.0	37.0
6	36.07603888764872	37.0	35.0	37.0	35.0	37.0
7	34.40121472049722	35.0	35.0	35.0	32.0	37.0
8	35.32968366434004	35.0	35.0	37.0	33.0	37.0
9	37.95874218037302	39.0	38.0	39.0	35.0	39.0
10	38.1347705568963	39.0	39.0	39.0	35.0	39.0
11	37.69069125590372	39.0	37.0	39.0	35.0	39.0
12	38.00727271448936	39.0	38.0	39.0	35.0	39.0
13	38.13127116067081	39.0	39.0	39.0	37.0	39.0
14	38.843040492440956	40.0	39.0	40.0	36.0	40.0
15	38.24056324164515	40.0	38.0	40.0	34.0	40.0
16	37.38408467959523	39.0	36.0	40.0	33.0	40.0
17	37.37572647168939	40.0	36.0	40.0	33.0	40.0
18	37.42062092979517	40.0	36.0	40.0	33.0	40.0
19	37.52742654955609	40.0	36.0	40.0	33.0	40.0
20	37.52418251391669	40.0	36.0	40.0	33.0	40.0
21	37.453115494066836	40.0	36.0	40.0	33.0	40.0
22	37.40291541356357	40.0	36.0	40.0	33.0	40.0
23	37.33258206323901	40.0	36.0	40.0	32.0	40.0
24	37.28307754503701	40.0	36.0	40.0	32.0	40.0
25	37.24400203191656	40.0	36.0	40.0	32.0	40.0
26	37.313654936274105	40.0	36.0	40.0	33.0	40.0
27	37.353309158916616	40.0	36.0	40.0	33.0	40.0
28	37.214779767313914	40.0	35.0	40.0	33.0	40.0
29	37.325551210097714	40.0	35.0	40.0	33.0	40.0
30	37.354782333575315	40.0	35.0	40.0	33.0	40.0
31	37.35603157556146	40.0	35.0	40.0	33.0	40.0
32	37.270925207542	40.0	35.0	40.0	33.0	40.0
33	37.18312904603627	40.0	35.0	40.0	33.0	40.0
34	37.10244431124884	40.0	35.0	40.0	33.0	40.0
35	36.92528516261484	39.0	35.0	40.0	32.0	40.0
36	36.46313129590927	39.0	35.0	40.0	31.0	40.0
37	36.51355172334999	39.0	35.0	40.0	31.0	40.0
38	36.370893182005936	39.0	35.0	40.0	31.0	40.0
39	36.31550482404411	38.0	35.0	40.0	31.0	40.0
40	36.20887659270795	38.0	35.0	40.0	31.0	40.0
41	36.10118550730242	38.0	35.0	40.0	31.0	40.0
42	35.79997257967275	37.0	35.0	40.0	30.0	40.0
43	35.59183886690771	37.0	35.0	40.0	30.0	40.0
44	35.4121637274771	37.0	35.0	40.0	30.0	40.0
45	35.01269666614521	36.0	34.0	40.0	28.0	40.0
46	35.00981950042273	36.0	34.0	40.0	29.0	40.0
47	34.91654039840329	35.0	34.0	39.0	29.0	40.0
48	34.7370038370881	35.0	34.0	39.0	29.0	40.0
49	34.574669171994	35.0	34.0	39.0	28.0	40.0
50	34.346448382112804	35.0	34.0	39.0	28.0	40.0
51	34.27018710857922	35.0	34.0	39.0	28.0	40.0
52	33.76147781502177	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	6.0
13	8.0
14	22.0
15	58.0
16	203.0
17	548.0
18	1455.0
19	3326.0
20	6402.0
21	11794.0
22	19564.0
23	30278.0
24	45180.0
25	65605.0
26	90985.0
27	121945.0
28	161767.0
29	211467.0
30	271587.0
31	341730.0
32	426500.0
33	554216.0
34	860451.0
35	1565178.0
36	1413974.0
37	2663042.0
38	4171352.0
39	1184381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.87972846845168	19.252121120507066	33.53934201760877	18.32880839343248
2	29.100047282487374	19.642600642268434	33.77927691190868	17.478075163335507
3	22.595256634928223	21.832598902132283	35.04220796912049	20.529936493819
4	22.068568395260503	25.053587404929683	40.16814988372727	12.709694316082548
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.57385296025282	0.3081412006236367	0.10681272092258855	0.011193118200945299
12	99.74393632859395	0.19252585156814392	0.04619973599146455	0.017338083846439138
13	99.24977984641102	0.38938973952446826	0.1069533379854145	0.25387707607910415
14	67.40313681512899	20.4215207383802	4.474631803009556	7.700710643481257
15	38.293942392704786	23.848063263616563	16.073781772864773	21.784212570813875
16	31.15596014209354	16.608696110707815	26.081230961767975	26.154112785430666
17	25.358065531066703	15.216791083472044	28.51233123755319	30.91281214790806
18	24.083744491765994	16.220121950147735	31.16256211319322	28.533571444893052
19	27.386529940009247	15.26342673235827	28.507072159403503	28.84297116822898
20	32.505019150286245	15.486016511958603	24.33248904505195	27.676475292703206
21	33.27989650584176	17.565974889308006	21.47283717774524	27.68129142710499
22	32.8658214409382	18.214346104292158	21.470095145020135	27.449737309749505
23	32.2034307047903	19.53455752204612	20.503191128469506	27.758820644694076
24	31.532356865012893	21.60050340908492	20.203958018775893	26.6631817071263
25	33.02426171647733	19.397807428447887	21.979269529512884	25.598661325561896
26	31.75103045941352	18.55414723661106	22.119619419919463	27.57520288405596
27	32.77017371480399	19.618302013812112	20.74171985214116	26.869804419242744
28	34.06157972723805	17.589886820841556	20.13998428604323	28.208549165877162
29	31.50557634539769	17.34572638380373	20.098228049237065	31.050469221561517
30	29.75937256666567	16.871551586248355	21.925546780660234	31.44352906642574
31	29.097952088251265	17.136530379437566	21.180100576354185	32.58541695595698
32	31.186459983020487	20.60731103263898	19.756950437758494	28.44927854658204
33	32.19147825445009	20.356436130851208	19.409844249025788	28.042241365672915
34	31.99055756423124	19.429158002604932	21.067642080359136	27.512642352804694
35	35.0271760051044	19.443050968412134	19.47484448631708	26.054928540166383
36	37.623536483975805	18.672167137440876	18.95368952807156	24.75060685051176
37	36.657194935676486	18.679528440679814	20.776149939974093	23.887126683669614
38	31.914090005466488	21.647631217691032	23.911636237720177	22.526642539122303
39	32.446276372290704	23.47382501261159	22.280731419652287	21.799167195445413
40	31.681945296447132	24.889360737255263	19.032041355478178	24.396652610819427
41	33.527980158932436	23.678387684757638	18.05030223879941	24.743329917510515
42	35.268067095431526	22.92074295025144	17.640944876353657	24.170245077963372
43	34.145099231703526	21.742702413867654	17.900727869071453	26.211470485357367
44	32.10371914554042	21.777603568861053	18.401500384060355	27.717176901538178
45	29.163430423556168	21.66171401653305	19.88839926808819	29.28645629182259
46	28.50525819929305	19.4942566718402	21.35175182494582	30.648733303920935
47	29.249797423543868	17.159725163950707	23.230402815153596	30.360074597351826
48	30.411202961395347	17.097347434881115	22.247974674866985	30.243474928856557
49	31.656317836747107	18.64092905693409	21.62307244766848	28.079680658650325
50	30.065207647458962	21.23408346677307	21.052054678944877	27.648654206823092
51	29.500468430590537	20.605335362906274	21.93740079905646	27.956795407446727
52	30.052207600000703	19.549273097670856	23.845293107478895	26.55322619484955
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	3.0
6	4.0
7	9.0
8	14.0
9	307.5
10	601.0
11	1089.5
12	1578.0
13	2280.0
14	3461.5
15	3941.0
16	4607.0
17	5273.0
18	6663.5
19	8054.0
20	10175.5
21	12297.0
22	15669.0
23	19041.0
24	24816.5
25	30592.0
26	35972.0
27	41352.0
28	44390.5
29	47429.0
30	49726.5
31	52024.0
32	55492.0
33	58960.0
34	62619.5
35	66279.0
36	76810.0
37	87341.0
38	92933.5
39	110073.5
40	121621.0
41	150843.5
42	180066.0
43	213103.5
44	246141.0
45	296679.5
46	347218.0
47	405203.5
48	463189.0
49	538392.5
50	613596.0
51	709486.0
52	805376.0
53	944073.5
54	1082771.0
55	1214118.5
56	1345466.0
57	1475841.5
58	1606217.0
59	1650635.5
60	1695054.0
61	1582453.0
62	1469852.0
63	1293934.5
64	970771.5
65	823526.0
66	722688.5
67	621851.0
68	534785.0
69	447719.0
70	393637.5
71	339556.0
72	266977.0
73	194398.0
74	149218.5
75	104039.0
76	72299.5
77	40560.0
78	27983.5
79	15407.0
80	9449.5
81	3492.0
82	2152.5
83	813.0
84	734.5
85	656.0
86	383.0
87	110.0
88	65.5
89	12.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1.4223025E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.223889720787961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.71321455751313	4.338873594875916
2	9.60747256149939	1.1959169943653687
3	4.676049203007687	0.8730964370549484
4	2.813851447108252	0.700524043899255
5	1.7727652393714814	0.5516747675347186
6	1.1574405669653929	0.43222694682953383
7	0.798617748275093	0.3479356156029725
8	0.5151705620984541	0.2565091812717699
9	0.3679351614972183	0.20609890826030955
>10	2.5534597797242946	3.379947493837853
>50	1.0583565350105872	4.9532071149120345
>100	4.571058526045242	65.71650946471456
>500	0.36542182353142044	14.634886624706853
>1k	0.029067644903890303	2.369520555373908
>5k	1.1864344858730735E-4	0.04307225676011333
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	7.452704329775136E-4	0.0	0.0	0.0	0.0
12	0.003248254151279352	0.0	0.0	0.0	0.0
13	0.004098987381376325	0.0	0.0	0.0	0.0
14	0.019039550306633082	0.0	0.0	0.0	0.0
15	0.037685372837353515	0.0	0.0	0.0	0.0
16	0.04930737307991795	0.0	0.0	0.0	0.0
17	0.05817327889109384	0.0	0.0	0.0	0.0
18	0.0660900195281946	0.0	0.0	0.0	0.0
19	0.07447079647262098	0.0	0.0	0.0	0.0
20	0.08467256438064336	0.0	0.0	0.0	0.0
21	0.09523993665201319	0.0	0.0	0.0	0.0
22	0.10691818371970802	0.0	0.0	0.0	0.0
23	0.12053694625440088	0.0	0.0	0.0	0.0
24	0.1339939991668439	0.0	0.0	0.0	0.0
25	0.14770416279237364	0.0	0.0	0.0	0.0
26	0.1634251504163144	0.0	0.0	0.0	0.0
27	0.1798210999418197	0.0	0.0	0.0	0.0
28	0.1959147227822492	0.0	0.0	0.0	0.0
29	0.21458866872553484	0.0	0.0	0.0	0.0
30	0.2344508288496997	0.0	0.0	0.0	0.0
31	0.25259746080738804	0.0	0.0	0.0	0.0
32	0.2686207751164046	0.0	0.0	0.0	0.0
33	0.28268248139899915	0.0	0.0	0.0	0.0
34	0.2968707430381371	0.0	0.0	0.0	0.0
35	0.3096458031958743	0.0	0.0	0.0	0.0
36	0.32195682704628587	0.0	0.0	0.0	0.0
37	0.3341412955401541	0.0	0.0	0.0	0.0
38	0.3484139274169876	0.0	0.0	0.0	0.0
39	0.3681073470657613	0.0	0.0	0.0	0.0
40	0.4001610065369357	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTA	18015	0.0	44.544544	2
ACCGTAT	52790	0.0	44.52737	3
TTACGTA	44955	0.0	44.48048	2
ATTACGT	8730	0.0	44.419247	1
CCGTATT	187350	0.0	44.374596	4
TTGGGGC	222650	0.0	44.349247	9
TACTCGT	8305	0.0	44.33835	1
GACGTAT	74340	0.0	44.326202	3
ATCGTAT	124435	0.0	44.3143	3
CGCGTAT	78185	0.0	44.29379	3
TCGTATT	594605	0.0	44.27908	4
TTCGTAT	225440	0.0	44.271736	3
AAATCGT	6240	0.0	44.267628	1
TTTCGTA	82960	0.0	44.261692	2
GCGCGTA	27825	0.0	44.255886	2
CGACGTA	14390	0.0	44.241833	2
AACGTAT	74640	0.0	44.24049	3
ATTCGTA	43785	0.0	44.22976	2
TCCGTAT	69785	0.0	44.226837	3
AAACGTA	16710	0.0	44.224415	2
>>END_MODULE