FastQCFastQC Report
Fri 17 Jun 2016
SRR1527952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13995547
Sequences flagged as poor quality0
Sequence length59
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA146400.051.6605222
AAATCGT62000.051.2048381
TTTCGTA799700.051.1641852
CGCGTAT783450.051.112583
GACGTAT746500.051.1008033
ATTACGT85750.051.0839651
CCGTATT1859950.051.079414
ACACGTA181700.051.060262
ACCGTAT524000.051.052963
TTACGTA447750.051.052822
TTGGGGC2171400.051.0424659
ATACGTA260750.050.9470752
TTCGTAT2193950.050.91163
AACGTAT762600.050.9115523
ATTCGTA424650.050.890732
ACGTATT3714400.050.886794
TCGTATT5721500.050.8309974
ATTGGGG9720950.050.8303228
CGTATTG14539500.050.7975465
TCCGTAT694200.050.797393
Produced by FastQC (version 0.11.5)