##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527952_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13995547
Sequences flagged as poor quality	0
Sequence length	59
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.020612341911324	31.0	31.0	33.0	30.0	33.0
2	31.598719506997476	31.0	31.0	34.0	30.0	34.0
3	32.00044892850562	31.0	31.0	34.0	30.0	34.0
4	35.792515933818095	37.0	35.0	37.0	35.0	37.0
5	35.872716157503525	37.0	35.0	37.0	35.0	37.0
6	35.84543598045864	37.0	35.0	37.0	35.0	37.0
7	33.92465832167903	35.0	33.0	35.0	31.0	37.0
8	34.95453282390463	35.0	35.0	37.0	33.0	37.0
9	37.665148564754205	39.0	37.0	39.0	35.0	39.0
10	37.88334110842541	39.0	37.0	39.0	35.0	39.0
11	36.824395502369434	37.0	37.0	39.0	34.0	39.0
12	37.6449777204135	39.0	37.0	39.0	35.0	39.0
13	37.84502284905334	39.0	37.0	39.0	35.0	39.0
14	38.29527470416126	40.0	38.0	40.0	35.0	40.0
15	37.57582794012981	40.0	37.0	40.0	33.0	40.0
16	36.666411252093255	38.0	35.0	40.0	31.0	40.0
17	36.548177002299376	39.0	35.0	40.0	31.0	40.0
18	36.50992997987146	39.0	35.0	40.0	31.0	40.0
19	36.77203527664906	39.0	35.0	40.0	31.0	40.0
20	36.90033222710052	39.0	35.0	40.0	31.0	40.0
21	36.82001067911101	39.0	35.0	40.0	31.0	40.0
22	36.79199734029688	39.0	35.0	40.0	31.0	40.0
23	36.743177526394646	39.0	35.0	40.0	31.0	40.0
24	36.67708321796926	39.0	35.0	40.0	31.0	40.0
25	36.64259860654249	39.0	35.0	40.0	31.0	40.0
26	36.51502002744159	39.0	35.0	40.0	30.0	40.0
27	36.41505258779811	39.0	35.0	40.0	30.0	40.0
28	36.42866870441005	39.0	35.0	40.0	30.0	40.0
29	36.39636864496972	39.0	35.0	40.0	30.0	40.0
30	36.35223896572246	39.0	35.0	40.0	30.0	40.0
31	36.33509186886372	39.0	35.0	40.0	30.0	40.0
32	36.18960902349869	38.0	35.0	40.0	30.0	40.0
33	36.02693549598312	38.0	35.0	40.0	30.0	40.0
34	35.92081974359416	38.0	35.0	40.0	30.0	40.0
35	35.76231732850456	38.0	34.0	40.0	29.0	40.0
36	35.57139838835881	38.0	34.0	40.0	29.0	40.0
37	35.374282548584915	38.0	34.0	40.0	28.0	40.0
38	35.03138141010137	37.0	33.0	40.0	26.0	40.0
39	34.9198499351258	37.0	33.0	40.0	26.0	40.0
40	34.78976227224273	37.0	33.0	40.0	26.0	40.0
41	34.68195798277838	37.0	33.0	40.0	26.0	40.0
42	34.56329831195594	36.0	33.0	40.0	26.0	40.0
43	34.317120224025544	36.0	33.0	40.0	26.0	40.0
44	34.12652653018849	35.0	33.0	39.0	25.0	40.0
45	33.923242728562165	35.0	33.0	39.0	24.0	40.0
46	33.73440838003688	35.0	33.0	39.0	24.0	40.0
47	33.58244411597489	35.0	33.0	39.0	23.0	40.0
48	33.36838724488582	35.0	32.0	38.0	23.0	40.0
49	33.229262636179925	35.0	32.0	38.0	23.0	40.0
50	33.00634015948073	35.0	32.0	38.0	23.0	40.0
51	32.8389684233135	35.0	32.0	37.0	23.0	40.0
52	32.6268900386673	35.0	31.0	37.0	23.0	40.0
53	32.415976095825336	35.0	31.0	37.0	23.0	39.0
54	32.26162021391519	35.0	31.0	36.0	22.0	39.0
55	32.10421443334798	35.0	31.0	36.0	22.0	39.0
56	31.903170344110166	34.0	31.0	36.0	21.0	39.0
57	31.74905539597702	34.0	31.0	35.0	21.0	39.0
58	31.53314779336599	34.0	31.0	35.0	20.0	38.0
59	31.006750575736696	34.0	30.0	35.0	20.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	6.0
13	19.0
14	75.0
15	310.0
16	1261.0
17	3469.0
18	7910.0
19	15754.0
20	27745.0
21	42709.0
22	61547.0
23	84449.0
24	112896.0
25	147900.0
26	187508.0
27	234710.0
28	286118.0
29	340448.0
30	407861.0
31	499943.0
32	620598.0
33	799701.0
34	1203220.0
35	1612546.0
36	1863169.0
37	2589849.0
38	2525513.0
39	318309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.563106536671985	19.654480099991805	33.225489507484056	18.55692385585215
2	28.164594066955722	20.301721683332563	33.823029567904705	17.710654681807007
3	22.652019245835834	22.020897075334034	35.20887036426657	20.118213314563555
4	22.350887750225127	25.52139619837653	39.35789004888483	12.769826002513513
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.55155021808008	0.32841874633410184	0.10412597664099875	0.01590505894482009
12	99.73283645147988	0.20032085919900094	0.04534299373936581	0.02149969558174468
13	99.2382648566719	0.3986267917931325	0.105512131823072	0.25759621971188407
14	67.2522267261151	20.503142892521456	4.517093901367343	7.727536479996101
15	38.405772921915805	23.91121261641292	16.005176503640765	21.67783795803051
16	31.283064534740944	16.70739271569736	25.93684977085926	26.072692978702438
17	25.371505665337697	15.275230042812904	28.387250601923597	30.966013689925802
18	23.99398894519807	16.258985804556264	31.133609854620186	28.61341539562548
19	27.466564900964567	15.306204180515417	28.50303743040554	28.72419348811447
20	32.610179509239615	15.564815008659542	24.309682215350353	27.515323266750492
21	33.281678808266655	17.624448690715695	21.51903030299566	27.574842198021987
22	32.82703419880623	18.290217595639525	21.49879529538931	27.383952910164925
23	32.19156064425349	19.710576514086945	20.51730453979398	27.580558301865587
24	31.53725252753608	21.795546826429863	20.161720009943163	26.505480636090894
25	32.94215653021636	19.603170922865683	21.990744627559035	25.46392791935892
26	31.694995558230055	18.63704934147983	22.27538516358096	27.392569936709155
27	32.713240861539745	19.720201003933607	20.83248336060034	26.73407477392631
28	34.05718976185783	17.58022748235564	20.154617750917488	28.207965004869052
29	31.480305842994206	17.380363911464126	20.108738872442785	31.030591373098886
30	29.68796432179464	16.912486521605764	21.968137436857596	31.431411719742
31	28.987820197381353	17.20189285920729	21.228487889755222	32.581799053656134
32	31.030855742901654	20.77778739194688	19.740071609919926	28.45128525523154
33	32.17080404217141	20.518254842057978	19.378413719735285	27.932527396035322
34	32.03023075839766	19.504711034159648	21.10936428565457	27.35569392178812
35	35.028298643847215	19.486698161922504	19.46250475240446	26.02249844182582
36	37.71955465549149	18.714674031675933	18.887871978137046	24.677899334695528
37	36.73355532298952	18.760124202362366	20.752665115554255	23.753655359093862
38	31.797399558588168	21.725846085186955	24.039503422052743	22.437250934172134
39	32.333091375421056	23.649422205505793	22.307602553869454	21.709883865203697
40	31.58973350595014	25.181702437210923	18.965318040088036	24.2632460167509
41	33.501327243586836	23.895900603241873	17.988471618865628	24.61430053430566
42	35.13027393641706	23.171563069310547	17.538221264234974	24.159941730037417
43	34.20215730046136	21.856566234960304	17.804677444904442	26.136599019673902
44	31.78417392332004	21.95176794447548	18.42981199663007	27.834246135574407
45	29.020266231823594	21.759914064094815	19.79516056071263	29.42465914336896
46	28.392116435320464	19.606479117965165	21.263370413460798	30.738034033253577
47	29.139111175861864	17.216690423032414	23.199178996004942	30.44501940510078
48	30.34836723423529	17.181221998682865	22.118056550415645	30.3523542166662
49	31.596099816605953	18.787711548537544	21.531212749312335	28.084975885544168
50	29.892779467640672	21.49423670257404	20.90185542587224	27.711128403913044
51	29.384367756401375	20.67678383703045	21.919614860355228	28.019233546212952
52	29.948904462255033	19.622434192818616	23.92678185425693	26.501879490669427
53	30.55970588359283	19.56663072904546	22.34229215907031	27.531371228291402
54	28.473785268985914	19.969144471452243	22.314383282053928	29.242686977507915
55	28.223141260573808	20.333317447328067	23.04582307501093	28.397718217087192
56	28.651377470276795	19.734069700884145	22.476427680890215	29.138125147948845
57	28.09999494839323	19.273873325565624	23.765694902814445	28.860436823226703
58	27.423522639022252	19.24835806703375	24.948814076363004	28.379305217580992
59	27.414891322218416	19.481532233073846	22.742012155723533	30.361564288984205
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	2.0
5	3.0
6	5.5
7	8.0
8	212.0
9	734.0
10	1052.0
11	1523.5
12	1995.0
13	2117.0
14	2414.0
15	2589.0
16	2949.0
17	3309.0
18	3512.0
19	4729.0
20	5743.0
21	7820.0
22	9897.0
23	13960.5
24	18024.0
25	22711.0
26	31149.5
27	34901.0
28	39680.5
29	44460.0
30	49243.0
31	56344.5
32	58663.0
33	62624.0
34	66585.0
35	71082.5
36	80389.0
37	85198.0
38	94530.5
39	103863.0
40	119975.5
41	136088.0
42	151282.5
43	188720.5
44	210964.0
45	235807.5
46	260651.0
47	305355.5
48	392997.5
49	435935.0
50	511748.0
51	587561.0
52	666190.0
53	868139.0
54	991459.0
55	1127382.0
56	1263305.0
57	1357015.5
58	1465993.0
59	1481260.0
60	1378238.5
61	1275217.0
62	1152637.0
63	1030057.0
64	950618.5
65	752194.0
66	633208.0
67	556151.5
68	479095.0
69	429658.0
70	332470.5
71	284720.0
72	235822.0
73	186924.0
74	141202.5
75	71446.5
76	47412.0
77	33032.5
78	18653.0
79	13486.5
80	8320.0
81	6014.0
82	2641.5
83	1575.0
84	1038.5
85	502.0
86	346.5
87	134.5
88	78.0
89	54.0
90	30.0
91	17.5
92	2.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1.3995547E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.155208944799243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.35680183511057	8.852417056616863
2	7.782017827268009	1.7362006975065452
3	3.3812682659369244	1.131562620148362
4	1.8881597782258785	0.842512673891019
5	1.0868672341171461	0.60621155459164
6	0.7414222019259932	0.4962431747278554
7	0.5068874670561946	0.39581049245583255
8	0.3311424302966464	0.2955170400358168
9	0.24278042923797932	0.24374397742719356
>10	1.4562493789889381	3.397174130857254
>50	0.587019436735015	4.93476917778021
>100	2.444892546275975	62.18378885010652
>500	0.18160713145951504	12.9960190628851
>1k	0.012819293458857635	1.8464289390993007
>5k	6.474390635641565E-5	0.041600551870559936
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	6.216262929916209E-4	0.0	0.0	0.0	0.0
12	0.002865197051605057	0.0	0.0	0.0	0.0
13	0.0037369028877542263	0.0	0.0	0.0	0.0
14	0.017862824511253473	0.0	0.0	0.0	0.0
15	0.034875378575771276	0.0	0.0	0.0	0.0
16	0.04571454048919989	0.0	0.0	0.0	0.0
17	0.05409577774988002	0.0	0.0	0.0	0.0
18	0.06129092346301292	0.0	0.0	0.0	0.0
19	0.06917914676718245	0.0	0.0	0.0	0.0
20	0.07833920317655323	0.0	0.0	0.0	0.0
21	0.08829951412402816	0.0	0.0	0.0	0.0
22	0.09909580525862977	0.0	0.0	0.0	0.0
23	0.11177841066161973	0.0	0.0	0.0	0.0
24	0.12459677353089522	0.0	0.0	0.0	0.0
25	0.13752945847704273	0.0	0.0	0.0	0.0
26	0.15119809179305388	0.0	0.0	0.0	0.0
27	0.16570270529619172	0.0	0.0	0.0	0.0
28	0.18065031684720861	0.0	0.0	0.0	0.0
29	0.1974628072772004	0.0	0.0	0.0	0.0
30	0.21592582269203198	0.0	0.0	0.0	0.0
31	0.23268829721339224	0.0	0.0	0.0	0.0
32	0.2475287318173416	0.0	0.0	0.0	0.0
33	0.26047570702309814	0.0	0.0	0.0	0.0
34	0.27297968418097557	0.0	0.0	0.0	0.0
35	0.28468340680074883	0.0	0.0	0.0	0.0
36	0.29565832618046295	0.0	0.0	0.0	0.0
37	0.30629742445936553	0.0	0.0	0.0	0.0
38	0.3194587535592571	0.0	0.0	0.0	0.0
39	0.33717153034461605	0.0	0.0	0.0	0.0
40	0.3657520495626216	0.0	0.0	0.0	0.0
41	0.3889808665570556	0.0	0.0	0.0	0.0
42	0.4059362595831374	0.0	0.0	0.0	0.0
43	0.42014077763448615	0.0	0.0	0.0	0.0
44	0.4332592359555507	0.0	0.0	0.0	0.0
45	0.44952155139059585	0.0	0.0	0.0	0.0
46	0.4665055249358957	0.0	0.0	0.0	7.145129804501389E-6
47	0.47960254786754675	0.0	0.0	0.0	7.145129804501389E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	14640	0.0	51.660522	2
AAATCGT	6200	0.0	51.204838	1
TTTCGTA	79970	0.0	51.164185	2
CGCGTAT	78345	0.0	51.11258	3
GACGTAT	74650	0.0	51.100803	3
ATTACGT	8575	0.0	51.083965	1
CCGTATT	185995	0.0	51.07941	4
ACACGTA	18170	0.0	51.06026	2
ACCGTAT	52400	0.0	51.05296	3
TTACGTA	44775	0.0	51.05282	2
TTGGGGC	217140	0.0	51.042465	9
ATACGTA	26075	0.0	50.947075	2
TTCGTAT	219395	0.0	50.9116	3
AACGTAT	76260	0.0	50.911552	3
ATTCGTA	42465	0.0	50.89073	2
ACGTATT	371440	0.0	50.88679	4
TCGTATT	572150	0.0	50.830997	4
ATTGGGG	972095	0.0	50.830322	8
CGTATTG	1453950	0.0	50.797546	5
TCCGTAT	69420	0.0	50.79739	3
>>END_MODULE