FastQCFastQC Report
Fri 17 Jun 2016
SRR1527951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085025
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTGCCTAGGGGGTGGGTGGATCACAAGGTCAGAAGTTCAAGATCAGCTTG13920.12829197483928942No Hit
GTTGGCCTAGGGAAGCAGTGGGGGAAAGGTTAACACTTCGGAGGCTGAGGAG12790.11787746826109997No Hit
CTTAGCCTAGGGGTGAGGCAGATGGACCACTTGAGGTCAGTTCAAGACCAGC12680.11686366673578949No Hit
GGTGGCCTAGGGGGATCACGAGGTCAAGAGGTCAAGACCATCCTGGCCAACG12290.11326927950968872No Hit
TTGGGCCTAGAGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTC12230.11271629685951938No Hit
GAATGCCTAGGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGG11730.10810810810810811No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG1050.046.00000416
CCTTCGA351.0195072E-746.00000421
GTGTTCA551.8189894E-1246.00000434
TACCCGT351.0195072E-746.00000439
GATGTTA351.0195072E-746.00000424
CATATAT351.0195072E-746.00000440
CCCTATT700.046.00000427
CTATCGC700.046.00000420
ATTACCC700.046.00000431
TATATTA351.0195072E-746.00000442
TACGGTT351.0195072E-746.00000437
CGATTCT351.0195072E-746.00000425
CGATTAC351.0195072E-746.00000429
TAACGAG700.046.00000420
ACTTAGG1100.046.00000423
TCCTAGT700.046.00000426
ATGTAGC351.0195072E-746.00000434
CGTTAGG351.0195072E-746.00000430
ACCTATG351.0195072E-746.00000415
ATATATT351.0195072E-746.00000441
Produced by FastQC (version 0.11.5)