##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527951_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1085025
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.678904172714915	31.0	31.0	34.0	27.0	34.0
2	31.606551922766755	31.0	31.0	34.0	28.0	34.0
3	31.84479435957697	33.0	31.0	34.0	30.0	34.0
4	35.35761480150227	37.0	35.0	37.0	33.0	37.0
5	35.61115734660492	37.0	35.0	37.0	33.0	37.0
6	35.37209096564595	37.0	35.0	37.0	32.0	37.0
7	35.85051127854197	37.0	35.0	37.0	35.0	37.0
8	35.8207396142946	37.0	35.0	37.0	35.0	37.0
9	37.16376949839866	39.0	37.0	39.0	33.0	39.0
10	37.45365590654593	39.0	37.0	39.0	34.0	39.0
11	38.07946084191609	39.0	38.0	39.0	35.0	39.0
12	38.12875555862768	39.0	39.0	39.0	37.0	39.0
13	37.91585263012373	39.0	38.0	39.0	35.0	39.0
14	38.07370244925232	40.0	38.0	40.0	33.0	40.0
15	37.086646851455036	39.0	36.0	40.0	31.0	40.0
16	37.265361627612265	39.0	36.0	40.0	32.0	40.0
17	37.018705559779725	39.0	36.0	40.0	31.0	40.0
18	37.16560355752171	39.0	36.0	40.0	32.0	40.0
19	37.34798829520057	39.0	36.0	40.0	32.0	40.0
20	36.95842031289602	39.0	36.0	40.0	32.0	40.0
21	37.025105412317686	39.0	36.0	40.0	32.0	40.0
22	37.25328909472132	39.0	36.0	40.0	32.0	40.0
23	36.66790903435405	39.0	35.0	40.0	30.0	40.0
24	37.18148798414783	39.0	36.0	40.0	32.0	40.0
25	37.433225040897675	39.0	37.0	40.0	32.0	40.0
26	37.307158821225315	40.0	36.0	40.0	32.0	40.0
27	37.2391898804175	39.0	36.0	40.0	32.0	40.0
28	36.894834681228545	39.0	35.0	40.0	31.0	40.0
29	36.792219534112114	39.0	35.0	40.0	31.0	40.0
30	36.55105458399576	39.0	35.0	40.0	31.0	40.0
31	36.31031174396903	39.0	35.0	40.0	30.0	40.0
32	36.22755789037119	39.0	35.0	40.0	30.0	40.0
33	36.15770051381305	39.0	35.0	40.0	30.0	40.0
34	35.85565586046405	39.0	35.0	40.0	29.0	40.0
35	35.7619483422041	39.0	35.0	40.0	28.0	40.0
36	35.20576392249026	38.0	35.0	40.0	25.0	40.0
37	34.93562821133153	38.0	34.0	40.0	24.0	40.0
38	34.62699845625677	38.0	34.0	40.0	22.0	40.0
39	34.22787954194604	38.0	33.0	40.0	21.0	40.0
40	33.70572659616138	37.0	33.0	40.0	18.0	40.0
41	33.35211907559734	36.0	33.0	40.0	17.0	40.0
42	32.91329232045344	35.0	33.0	40.0	15.0	40.0
43	32.546758830441696	35.0	32.0	40.0	14.0	40.0
44	32.23580101840972	35.0	31.0	40.0	12.0	40.0
45	31.778426303541394	35.0	31.0	39.0	10.0	40.0
46	31.465558858090827	35.0	30.0	39.0	10.0	40.0
47	31.019472362387962	35.0	28.0	39.0	10.0	40.0
48	30.488434828690583	35.0	26.0	38.0	9.0	40.0
49	30.013347157899588	35.0	26.0	38.0	9.0	40.0
50	29.395538351650885	35.0	23.0	37.0	8.0	40.0
51	28.970693762816524	35.0	22.0	37.0	8.0	40.0
52	28.273195548489667	34.0	20.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	6.0
15	13.0
16	35.0
17	98.0
18	302.0
19	642.0
20	1468.0
21	2578.0
22	4289.0
23	6864.0
24	10235.0
25	14532.0
26	20002.0
27	25409.0
28	31759.0
29	37781.0
30	41047.0
31	42237.0
32	44033.0
33	51741.0
34	69102.0
35	97619.0
36	108903.0
37	193606.0
38	220333.0
39	60387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.507131172092812	22.533858666850996	38.92269763369507	8.03631252736112
2	27.140204142761686	20.73998294970162	34.173129651390525	17.946683256146173
3	26.381327619179277	22.152669293334252	32.72108937582083	18.74491371166563
4	25.869726503997605	21.173797838759477	38.41487523328956	14.541600423953366
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	95.74313955899633	3.8115250800672795	0.2740950669339416	0.17124029400244234
12	96.6458837353978	1.6505610469804846	1.0631091449505772	0.6404460726711366
13	91.36029123752908	5.098868689661529	1.6945231676689476	1.8463169051404345
14	36.633902444644136	46.34354047141771	7.853183106380038	9.16937397755812
15	21.998571461487064	57.211308495195965	11.800926245939033	8.989193797377942
16	48.870947674016726	28.352802930808046	12.515932812608005	10.260316582567222
17	17.78935969217299	27.235962305016017	25.409921430381786	29.564756572429207
18	19.182691643049697	42.910071196516206	23.68839427662957	14.21884288380452
19	42.72104329393332	28.111702495334207	20.475933734245753	8.691320476486716
20	20.74035160480173	34.4411419091726	33.77710191009424	11.04140457593143
21	36.48321467247298	25.774613488168473	22.53183106380037	15.210340775558167
22	43.742033593696	28.068201193520885	14.945830741227162	13.243934471555955
23	17.65231215870602	26.728785051035693	28.025529365682818	27.593373424575468
24	20.023962581507337	40.16110227874934	13.479781571853183	26.335153567890142
25	18.842883804520632	27.05016013455911	36.05612773899219	18.050828321928066
26	16.622197645215547	39.188497960876475	14.197276560447916	29.99202783346006
27	30.973111218635513	42.48086449620976	13.702080597221261	12.843943687933457
28	32.069767977696365	41.73737932305707	12.855279832261928	13.337572866984631
29	33.59876500541462	28.892698324923387	11.337618948872146	26.170917720789845
30	42.48501186608603	30.873021358954862	12.90772102025299	13.734245754706112
31	23.08306260224419	39.06343171816318	13.786779106472203	24.066726573120434
32	21.972857768254187	53.42549710836156	12.697311121863553	11.904334001520702
33	31.96000092163775	44.63482408239442	11.0226953296007	12.382479666367134
34	18.4068569848621	55.17541070482247	12.284786064837215	14.132946245478214
35	27.302228059261306	45.051404345521995	14.008340821640056	13.638026773576645
36	17.35858620769107	45.3923181493514	24.00313356835096	13.245962074606577
37	19.20287550978088	48.715467385544116	17.614248519619363	14.467408585055644
38	21.443837699592176	46.85403562129905	17.037303287942674	14.6648233911661
39	20.691228312711686	47.44286997995438	16.536024515564158	15.329877191769775
40	22.83477339231815	46.34538374691827	16.269210386857445	14.55063247390613
41	29.019700006912284	42.83910508974448	13.453791387295224	14.687403516048017
42	18.85661620699984	44.63786548697035	12.516670122808229	23.988848183221585
43	18.350821409644936	44.19704615101034	14.457547061127624	22.994585378217092
44	18.214787677703278	45.7152600170503	22.933019976498237	13.136932328748186
45	19.515402870901593	53.131126010921406	14.552475749406696	12.800995368770304
46	29.032695099191265	44.667450058754405	13.696182115619457	12.603672726434874
47	30.341697195917146	44.75472915370614	12.955185364392527	11.948388285984194
48	27.87180018893574	45.571484528006266	13.189281352964215	13.367433930093778
49	25.746687864334923	47.71779452086358	13.396557683002698	13.138959931798807
50	19.994562337273337	52.30358747494297	14.788507177253981	12.913343010529713
51	19.793737471486832	52.54219948849105	14.36943849220064	13.29462454782148
52	23.548950485011865	48.2586115527292	13.198682058017097	14.993755904241837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	4.5
10	8.0
11	129.0
12	250.0
13	865.0
14	2851.5
15	4223.0
16	4944.0
17	5665.0
18	5021.5
19	4378.0
20	4210.0
21	4042.0
22	4708.5
23	5375.0
24	6795.0
25	8215.0
26	10530.0
27	12845.0
28	15991.0
29	19137.0
30	23444.0
31	27751.0
32	33962.0
33	40173.0
34	47866.5
35	55560.0
36	58377.5
37	61195.0
38	57992.0
39	49782.5
40	44776.0
41	41397.5
42	38019.0
43	38554.0
44	39089.0
45	38949.0
46	38809.0
47	41009.0
48	43209.0
49	49632.0
50	56055.0
51	63945.0
52	71835.0
53	83423.5
54	95012.0
55	97313.5
56	99615.0
57	93764.0
58	87913.0
59	74428.0
60	60943.0
61	50038.0
62	39133.0
63	33860.0
64	21797.5
65	15008.0
66	11738.5
67	8469.0
68	5939.5
69	3410.0
70	3571.0
71	3732.0
72	3293.5
73	2855.0
74	2371.0
75	1887.0
76	1161.5
77	436.0
78	710.0
79	984.0
80	544.0
81	104.0
82	61.0
83	18.0
84	14.5
85	11.0
86	8.0
87	5.0
88	14.0
89	12.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1085025.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.417395992600014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.87377496431719	37.53950037935132
2	7.9649910439221285	7.39428286726492
3	3.0669453815432646	4.270788547883083
4	1.7463056158733787	3.242358371843835
5	1.154235190665842	2.678829595686528
6	0.8065641021285788	2.246316319315087
7	0.6039284547962243	1.9622950366232763
8	0.4535323273249164	1.6841431706308905
9	0.40196232644763424	1.679224003274394
>10	2.7312671657523815	23.08581849650419
>50	0.11171816683273172	3.507097595708106
>100	0.07579905645745688	7.659267704159649
>500	0.007779376746559799	2.349732173046945
>1k	0.0011968271917784307	0.7003457387078214
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CACTGCCTAGGGGGTGGGTGGATCACAAGGTCAGAAGTTCAAGATCAGCTTG	1392	0.12829197483928942	No Hit
GTTGGCCTAGGGAAGCAGTGGGGGAAAGGTTAACACTTCGGAGGCTGAGGAG	1279	0.11787746826109997	No Hit
CTTAGCCTAGGGGTGAGGCAGATGGACCACTTGAGGTCAGTTCAAGACCAGC	1268	0.11686366673578949	No Hit
GGTGGCCTAGGGGGATCACGAGGTCAAGAGGTCAAGACCATCCTGGCCAACG	1229	0.11326927950968872	No Hit
TTGGGCCTAGAGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTC	1223	0.11271629685951938	No Hit
GAATGCCTAGGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGG	1173	0.10810810810810811	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.676205617382088	0.0	0.0	0.0	0.0
10	0.676205617382088	0.0	0.0	0.0	0.0
11	0.6883712356858137	0.0	0.0	0.0	0.0
12	0.7395221308264787	0.0	0.0	0.0	0.0
13	0.8670767954655423	0.0	0.0	0.0	0.0
14	1.5556323587014125	0.0	0.0	0.0	0.0
15	1.9940554365106795	0.0	0.0	0.0	0.0
16	2.275523605446879	0.0	0.0	0.0	0.0
17	2.57146148706251	0.0	0.0	0.0	0.0
18	3.280753899679731	0.0	0.0	0.0	0.0
19	3.523881938204189	0.0	0.0	0.0	0.0
20	3.6800073731020024	0.0	0.0	0.0	0.0
21	3.853459597705122	0.0	0.0	0.0	0.0
22	4.014377548904403	0.0	0.0	0.0	0.0
23	4.167461579226285	0.0	0.0	0.0	0.0
24	4.386442708693348	0.0	0.0	0.0	0.0
25	4.590585470380867	0.0	0.0	0.0	0.0
26	4.884772240270961	0.0	0.0	0.0	0.0
27	5.121540978318472	0.0	0.0	0.0	0.0
28	5.425220617036474	0.0	0.0	0.0	0.0
29	5.618488053270662	0.0	0.0	0.0	0.0
30	5.899403239556692	0.0	0.0	0.0	0.0
31	6.202529895624525	0.0	0.0	0.0	0.0
32	6.825557014815327	0.0	0.0	0.0	0.0
33	7.067302596714361	0.0	0.0	0.0	0.0
34	7.388401188912698	0.0	0.0	0.0	0.0
35	7.587382779198636	0.0	0.0	0.0	0.0
36	7.822953388170779	0.0	0.0	0.0	0.0
37	8.047925163014677	0.0	0.0	0.0	0.0
38	8.25492500172807	0.0	0.0	0.0	0.0
39	8.504596668279532	0.0	0.0	0.0	0.0
40	8.790027879541945	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	105	0.0	46.000004	16
CCTTCGA	35	1.0195072E-7	46.000004	21
GTGTTCA	55	1.8189894E-12	46.000004	34
TACCCGT	35	1.0195072E-7	46.000004	39
GATGTTA	35	1.0195072E-7	46.000004	24
CATATAT	35	1.0195072E-7	46.000004	40
CCCTATT	70	0.0	46.000004	27
CTATCGC	70	0.0	46.000004	20
ATTACCC	70	0.0	46.000004	31
TATATTA	35	1.0195072E-7	46.000004	42
TACGGTT	35	1.0195072E-7	46.000004	37
CGATTCT	35	1.0195072E-7	46.000004	25
CGATTAC	35	1.0195072E-7	46.000004	29
TAACGAG	70	0.0	46.000004	20
ACTTAGG	110	0.0	46.000004	23
TCCTAGT	70	0.0	46.000004	26
ATGTAGC	35	1.0195072E-7	46.000004	34
CGTTAGG	35	1.0195072E-7	46.000004	30
ACCTATG	35	1.0195072E-7	46.000004	15
ATATATT	35	1.0195072E-7	46.000004	41
>>END_MODULE