FastQCFastQC Report
Fri 17 Jun 2016
SRR1527950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083175
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTGCCTAGGGGGTGGGTGGATCACAAGGTCAGAAGTTCAAGATCAGCTTG13970.12897269600941674No Hit
GGTGGCCTAGGGGGATCACGAGGTCAAGAGGTCAAGACCATCCTGGCCAACG12360.11410898515936946No Hit
GTTGGCCTAGGGAAGCAGTGGGGGAAAGGTTAACACTTCGGAGGCTGAGGAG12180.11244720382209707No Hit
CTTAGCCTAGGGGTGAGGCAGATGGACCACTTGAGGTCAGTTCAAGACCAGC11770.10866203522053224No Hit
TTGGGCCTAGAGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTC11450.10570775728760357No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC351.019489E-746.00000445
TCCGCCG351.019489E-746.00000420
TACCATC700.046.00000441
CTATACA351.019489E-746.00000446
TAGAACA351.019489E-746.00000415
CCGGATA351.019489E-746.00000424
TTCGGTA351.019489E-746.00000439
CTACAAC351.019489E-746.00000440
GAACAAT351.019489E-746.00000435
TTACCCT351.019489E-746.00000432
TGAGAGT351.019489E-746.00000444
TCGGTAC351.019489E-746.00000440
TCAACTA700.046.00000425
CCCGACC351.019489E-746.00000434
CGGACAG206.311292E-446.033
GAAATTG206.311292E-446.043
TTCTAAT650.046.024
ACTATAA253.417024E-546.039
CTCACGC1000.046.039
TCGTTAG206.311292E-446.029
Produced by FastQC (version 0.11.5)