##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527950_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1083175
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.591233180234035	31.0	31.0	34.0	26.0	34.0
2	31.42559050938214	31.0	31.0	34.0	28.0	34.0
3	31.771273339949683	33.0	31.0	34.0	30.0	34.0
4	35.329027165508805	37.0	35.0	37.0	33.0	37.0
5	35.60453758626261	37.0	35.0	37.0	33.0	37.0
6	35.362993052830795	37.0	35.0	37.0	32.0	37.0
7	35.85276986636509	37.0	35.0	37.0	35.0	37.0
8	35.78143790246267	37.0	35.0	37.0	35.0	37.0
9	37.158875527961776	39.0	37.0	39.0	33.0	39.0
10	37.44562789946223	39.0	37.0	39.0	34.0	39.0
11	38.07136427631731	39.0	38.0	39.0	35.0	39.0
12	38.11907124887484	39.0	39.0	39.0	37.0	39.0
13	37.92164424031205	39.0	38.0	39.0	35.0	39.0
14	38.10631430747571	40.0	38.0	40.0	33.0	40.0
15	37.131268723890415	39.0	36.0	40.0	31.0	40.0
16	37.31668474623214	39.0	36.0	40.0	32.0	40.0
17	37.09719297435779	39.0	36.0	40.0	32.0	40.0
18	37.25834883559905	39.0	36.0	40.0	32.0	40.0
19	37.440301890276274	39.0	37.0	40.0	33.0	40.0
20	37.0283813788169	39.0	36.0	40.0	32.0	40.0
21	37.289089020703024	39.0	36.0	40.0	32.0	40.0
22	37.41421930897593	39.0	36.0	40.0	32.0	40.0
23	36.79763842407736	39.0	35.0	40.0	31.0	40.0
24	37.305968103030445	39.0	36.0	40.0	32.0	40.0
25	37.38135019733653	40.0	36.0	40.0	32.0	40.0
26	37.22327601726406	39.0	36.0	40.0	32.0	40.0
27	37.22294827705588	39.0	36.0	40.0	32.0	40.0
28	36.722143697925084	39.0	35.0	40.0	31.0	40.0
29	36.71915618436541	39.0	35.0	40.0	31.0	40.0
30	36.561478985390174	39.0	35.0	40.0	31.0	40.0
31	36.363165693447506	39.0	35.0	40.0	30.0	40.0
32	36.276881390357055	39.0	35.0	40.0	30.0	40.0
33	36.19096914164378	39.0	35.0	40.0	30.0	40.0
34	35.92135527500173	39.0	35.0	40.0	30.0	40.0
35	35.77152906963325	39.0	35.0	40.0	28.0	40.0
36	34.835152214554434	38.0	34.0	40.0	24.0	40.0
37	34.79458720889976	38.0	34.0	40.0	23.0	40.0
38	34.59272139774275	38.0	34.0	40.0	22.0	40.0
39	34.23396865695756	38.0	33.0	40.0	21.0	40.0
40	33.72012094075288	37.0	33.0	40.0	18.0	40.0
41	33.405789461536685	36.0	33.0	40.0	17.0	40.0
42	32.821044614212845	35.0	33.0	40.0	15.0	40.0
43	32.47093221316962	35.0	32.0	40.0	14.0	40.0
44	32.17172756018187	35.0	31.0	40.0	12.0	40.0
45	31.50378470699564	35.0	30.0	39.0	10.0	40.0
46	31.332376578115262	35.0	30.0	39.0	10.0	40.0
47	30.986873773859255	35.0	28.0	39.0	10.0	40.0
48	30.47372954508736	35.0	26.0	38.0	9.0	40.0
49	30.01333764165532	35.0	26.0	38.0	9.0	40.0
50	29.316803840561313	35.0	23.0	37.0	8.0	40.0
51	28.98798070487225	35.0	22.0	37.0	8.0	40.0
52	28.37389526161516	34.0	20.0	36.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	6.0
15	13.0
16	31.0
17	116.0
18	273.0
19	644.0
20	1412.0
21	2481.0
22	4262.0
23	6860.0
24	10218.0
25	14545.0
26	19461.0
27	25562.0
28	32216.0
29	37995.0
30	41271.0
31	41797.0
32	44497.0
33	52148.0
34	69133.0
35	97308.0
36	110834.0
37	194062.0
38	215013.0
39	61012.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.463960117247908	22.64047822374039	38.770558773974656	8.125002885037045
2	27.33916495487802	20.834121910125326	33.92951277494403	17.897200360052622
3	26.381148013940496	22.083042906270915	32.87418930459067	18.661619775197913
4	25.844484963186925	21.173402266485102	38.37865534193459	14.60345742839338
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	95.78092182703625	3.7751979135412097	0.26773143767165974	0.17614882175087127
12	96.65695755533501	1.672952200706257	1.036028342603919	0.6340619013548133
13	91.3228241050615	5.124656680591779	1.7361922127080112	1.8163270016387012
14	36.51127472476747	46.48611720174487	7.850347358460083	9.15226071502758
15	21.975488725275234	57.15595356244374	11.849608788976852	9.018948923304174
16	48.861910586931934	28.328940383594524	12.526600041544533	10.282548987929005
17	17.828882682853646	27.186096429478155	25.38620259884137	29.598818288826827
18	19.11934821243105	42.993145151983754	23.636900777805987	14.250605857779213
19	42.74618598102799	28.095367784522352	20.472684469268586	8.685761765181065
20	20.769958686269533	34.32815565351859	33.82657465321855	11.07531100699333
21	36.24705149214116	25.96293304406029	22.555173448427077	15.234842015371477
22	43.738915687677434	28.025665289542317	14.997761211253952	13.237657811526299
23	17.675352551526764	26.687746670667252	28.09305975488725	27.543841022918734
24	19.921157707664968	40.22461744408798	13.467537563182312	26.386687285064742
25	18.883375262538372	27.07272601380202	36.02077226671591	18.023126456943707
26	16.72472130542156	39.109839130334436	14.131511528608026	30.033928035635977
27	30.963787015025275	42.63263092298105	13.610450758187733	12.793131303805941
28	32.19341288342142	41.585801001684864	12.922934890483994	13.29785122440972
29	33.640916749371065	28.860802732707086	11.360860433447966	26.137420084473884
30	42.53643224779006	30.840353590140097	12.851293650610474	13.771920511459367
31	23.135412098691347	39.00302351882198	13.739146490640941	24.122417891845732
32	22.000046160592703	53.40503612066379	12.635446719135873	11.959470999607635
33	31.921803955962798	44.686408013478896	11.079557781521915	12.312230249036398
34	18.462759941837653	55.204283703002744	12.190273963117686	14.142682392041914
35	27.476446557573798	44.94786161054308	13.948530939137257	13.627160892745863
36	17.59678722274794	44.70681099545318	24.063701617928775	13.632700163870105
37	19.250167332148546	48.57913079603942	17.707572645232762	14.46312922657927
38	21.44750386594964	46.80619475154061	17.02984282318185	14.716458559327902
39	20.778267592955892	47.30002077226671	16.61292034989729	15.308791284880098
40	22.791423361875967	46.33092528908071	16.285734068825445	14.591917280217878
41	29.076741985367093	42.87497403466661	13.379740115863086	14.668543864103215
42	19.136981558843217	44.27243981812726	12.63766242758557	23.952916195443947
43	18.30036697671198	44.133404112908806	14.5534193459044	23.012809564474807
44	18.421492371962056	45.48295520114478	22.857063724698225	13.238488702194937
45	19.81212638770282	52.642740092782795	14.688023634223462	12.857109885290926
46	29.07415699217578	44.593717543333256	13.758533939575784	12.573591524915178
47	30.262792254252545	44.7530639093406	12.974173148383224	12.009970688023634
48	28.144851939898906	45.46190597087267	13.13776628891915	13.255475800309277
49	25.838207122579455	47.65536501488679	13.503450504304476	13.002977358229279
50	20.156669051630622	52.13654303321255	14.77018948923304	12.93659842592379
51	19.664597133427193	52.641632238557946	14.402658850139636	13.291111777875228
52	23.589724652064533	48.20772266715905	13.268585408636646	14.933967272139775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	3.0
10	6.0
11	117.5
12	229.0
13	868.0
14	2744.0
15	3981.0
16	4901.0
17	5821.0
18	5157.0
19	4493.0
20	4308.0
21	4123.0
22	4735.5
23	5348.0
24	6591.5
25	7835.0
26	10196.0
27	12557.0
28	15804.5
29	19052.0
30	23252.0
31	27452.0
32	33432.5
33	39413.0
34	47243.5
35	55074.0
36	58182.5
37	61291.0
38	57844.5
39	49666.5
40	44935.0
41	41608.5
42	38282.0
43	38543.0
44	38804.0
45	38732.0
46	38660.0
47	40895.5
48	43131.0
49	49743.0
50	56355.0
51	64197.5
52	72040.0
53	83268.5
54	94497.0
55	97043.5
56	99590.0
57	93957.0
58	88324.0
59	74660.0
60	60996.0
61	50224.5
62	39453.0
63	33833.0
64	21712.0
65	15211.0
66	11866.5
67	8522.0
68	6000.0
69	3478.0
70	3586.5
71	3695.0
72	3316.0
73	2937.0
74	2405.5
75	1874.0
76	1171.0
77	468.0
78	708.0
79	948.0
80	533.5
81	119.0
82	69.0
83	19.0
84	21.0
85	23.0
86	14.0
87	5.0
88	9.5
89	7.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1083175.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.29603775192447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.82606274187334	38.22752514779057
2	8.001827925853055	7.569095113310992
3	3.1618340262726496	4.486266644157317
4	1.7642893096097683	3.3377557517048144
5	1.1521631314757212	2.7246375481325615
6	0.7969771181967032	2.261631592179075
7	0.6121008485849413	2.0264961389860874
8	0.4773855186375701	1.806275480936365
9	0.39525506709408914	1.682459871742933
>10	2.6343116640312454	22.492116432764135
>50	0.09348652410384022	2.9690591350760744
>100	0.07673252458166642	7.796438950889903
>500	0.006377768156168924	1.9374917444736393
>1k	0.0011958315292816732	0.6827504478555302
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CACTGCCTAGGGGGTGGGTGGATCACAAGGTCAGAAGTTCAAGATCAGCTTG	1397	0.12897269600941674	No Hit
GGTGGCCTAGGGGGATCACGAGGTCAAGAGGTCAAGACCATCCTGGCCAACG	1236	0.11410898515936946	No Hit
GTTGGCCTAGGGAAGCAGTGGGGGAAAGGTTAACACTTCGGAGGCTGAGGAG	1218	0.11244720382209707	No Hit
CTTAGCCTAGGGGTGAGGCAGATGGACCACTTGAGGTCAGTTCAAGACCAGC	1177	0.10866203522053224	No Hit
TTGGGCCTAGAGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTC	1145	0.10570775728760357	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.6590809425993029	0.0	0.0	0.0	0.0
10	0.6590809425993029	0.0	0.0	0.0	0.0
11	0.6693285941791492	0.0	0.0	0.0	0.0
12	0.7208438156345928	0.0	0.0	0.0	0.0
13	0.8513859717958778	0.0	0.0	0.0	0.0
14	1.5578276825074433	0.0	0.0	0.0	0.0
15	2.0072472130542156	0.0	0.0	0.0	0.0
16	2.29482770558774	0.0	0.0	0.0	0.0
17	2.6071502758095413	0.0	0.0	0.0	0.0
18	3.3195928635723684	0.0	0.0	0.0	0.0
19	3.5618436540725185	0.0	0.0	0.0	0.0
20	3.7185127057031413	0.0	0.0	0.0	0.0
21	3.8989083019825976	0.0	0.0	0.0	0.0
22	4.062132157776906	0.0	0.0	0.0	0.0
23	4.217324070441064	0.0	0.0	0.0	0.0
24	4.4477577492095	0.0	0.0	0.0	0.0
25	4.640893669074711	0.0	0.0	0.0	0.0
26	4.943614836014494	0.0	0.0	0.0	0.0
27	5.183649918064948	0.0	0.0	0.0	0.0
28	5.474830936829229	0.0	0.0	0.0	0.0
29	5.669628638031712	0.0	0.0	0.0	0.0
30	5.9570244881944285	0.0	0.0	0.0	0.0
31	6.251621390818658	0.0	0.0	0.0	0.0
32	6.875343319408222	0.0	0.0	0.0	0.0
33	7.119809818358068	0.0	0.0	0.0	0.0
34	7.429639716573961	0.0	0.0	0.0	0.0
35	7.619544394950031	0.0	0.0	0.0	0.0
36	7.845823620375286	0.0	0.0	0.0	0.0
37	8.052807718051099	0.0	0.0	0.0	0.0
38	8.25674521660858	0.0	0.0	0.0	0.0
39	8.511274724767466	0.0	0.0	0.0	0.0
40	8.79654718766589	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATAC	35	1.019489E-7	46.000004	45
TCCGCCG	35	1.019489E-7	46.000004	20
TACCATC	70	0.0	46.000004	41
CTATACA	35	1.019489E-7	46.000004	46
TAGAACA	35	1.019489E-7	46.000004	15
CCGGATA	35	1.019489E-7	46.000004	24
TTCGGTA	35	1.019489E-7	46.000004	39
CTACAAC	35	1.019489E-7	46.000004	40
GAACAAT	35	1.019489E-7	46.000004	35
TTACCCT	35	1.019489E-7	46.000004	32
TGAGAGT	35	1.019489E-7	46.000004	44
TCGGTAC	35	1.019489E-7	46.000004	40
TCAACTA	70	0.0	46.000004	25
CCCGACC	35	1.019489E-7	46.000004	34
CGGACAG	20	6.311292E-4	46.0	33
GAAATTG	20	6.311292E-4	46.0	43
TTCTAAT	65	0.0	46.0	24
ACTATAA	25	3.417024E-5	46.0	39
CTCACGC	100	0.0	46.0	39
TCGTTAG	20	6.311292E-4	46.0	29
>>END_MODULE