FastQCFastQC Report
Fri 17 Jun 2016
SRR1527949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107348
Sequences flagged as poor quality0
Sequence length59
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTGCCTAGGGGGTGGGTGGATCACAAGGTCAGAAGTTCAAGATCAGCTTGGTCAACA14640.13220776124578723No Hit
GGTGGCCTAGGGGGATCACGAGGTCAAGAGGTCAAGACCATCCTGGCCAACGTGGTGAA12410.1120695571762445No Hit
TTGGGCCTAGAGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGGAGGCT12340.11143741624132612No Hit
GAATGCCTAGGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCAGG11680.10547723028352424No Hit
GTTGGCCTAGGGAAGCAGTGGGGGAAAGGTTAACACTTCGGAGGCTGAGGAGGGTAGAT11570.10448386595722392No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGT150.00669620153.00000452
CTCGTCG150.00669620153.00000442
CAAGTAA150.00669620153.00000426
CTATATT307.0102396E-753.00000448
CTCCCGA150.00669620153.00000418
ACCGACT307.0102396E-753.00000446
TAAACAC750.053.00000441
CAACATA150.00669620153.00000426
TACATGA150.00669620153.00000443
CTGCGAC307.0102396E-753.00000448
CTAACTA150.00669620153.00000428
ACGATGC150.00669620153.00000431
CCACTAT900.053.00000417
CACTCAC600.053.00000448
TCTAATA150.00669620153.00000453
CGTACTA150.00669620153.00000449
CGAGCTA457.6397555E-1153.00000421
TACTCGA150.00669620153.00000416
CCCCACG150.00669620153.00000428
TCCCGGA307.0102396E-753.00000432