##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527949_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1107348
Sequences flagged as poor quality	0
Sequence length	59
%GC	46
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.443570584856793	31.0	31.0	34.0	26.0	34.0
2	31.33344079729227	31.0	31.0	34.0	28.0	34.0
3	31.601463135346794	31.0	31.0	34.0	30.0	34.0
4	35.195013672305365	37.0	35.0	37.0	33.0	37.0
5	35.339663773267304	37.0	35.0	37.0	33.0	37.0
6	35.02054277426789	37.0	35.0	37.0	32.0	37.0
7	35.67882725213754	37.0	35.0	37.0	33.0	37.0
8	35.52820071016519	37.0	35.0	37.0	33.0	37.0
9	36.8966928192402	39.0	37.0	39.0	33.0	39.0
10	37.1371321391288	39.0	37.0	39.0	33.0	39.0
11	37.916009240094354	39.0	37.0	39.0	35.0	39.0
12	37.93954565321832	39.0	37.0	39.0	35.0	39.0
13	37.67912255226	39.0	37.0	39.0	35.0	39.0
14	37.51058836065988	40.0	37.0	40.0	32.0	40.0
15	36.53127833345976	38.0	35.0	40.0	31.0	40.0
16	36.77080827346056	39.0	36.0	40.0	31.0	40.0
17	36.31352203643299	38.0	35.0	40.0	30.0	40.0
18	36.490337274280535	38.0	35.0	40.0	30.0	40.0
19	36.7040867008384	39.0	35.0	40.0	31.0	40.0
20	36.43048978279637	39.0	35.0	40.0	30.0	40.0
21	36.66254691388796	39.0	35.0	40.0	31.0	40.0
22	36.77292684865101	39.0	35.0	40.0	31.0	40.0
23	36.26979142961382	39.0	35.0	40.0	30.0	40.0
24	36.71956602621759	39.0	35.0	40.0	31.0	40.0
25	36.764708113438594	39.0	35.0	40.0	31.0	40.0
26	36.512996817621925	39.0	35.0	40.0	30.0	40.0
27	36.394598626628664	39.0	35.0	40.0	30.0	40.0
28	36.05262121753956	38.0	35.0	40.0	30.0	40.0
29	35.93312400437803	38.0	35.0	40.0	30.0	40.0
30	35.6155210466809	38.0	35.0	40.0	28.0	40.0
31	35.41492737603716	38.0	34.0	40.0	27.0	40.0
32	35.24775680273952	38.0	34.0	40.0	26.0	40.0
33	35.115560781253954	38.0	34.0	40.0	25.0	40.0
34	34.83319426232765	38.0	33.0	40.0	25.0	40.0
35	34.759939964672355	38.0	33.0	40.0	24.0	40.0
36	34.03301491491383	37.0	33.0	40.0	22.0	40.0
37	33.803502602614536	37.0	33.0	40.0	21.0	40.0
38	33.5162460220274	37.0	32.0	40.0	19.0	40.0
39	33.13087936222398	37.0	32.0	40.0	16.0	40.0
40	32.57650801735317	35.0	31.0	40.0	15.0	40.0
41	32.26556060064225	35.0	31.0	39.0	15.0	40.0
42	31.863321196227382	35.0	30.0	39.0	13.0	40.0
43	31.45985453534029	35.0	30.0	39.0	12.0	40.0
44	31.15119998410617	35.0	29.0	38.0	10.0	40.0
45	30.666999895245215	35.0	27.0	38.0	10.0	40.0
46	30.295582779758487	35.0	26.0	38.0	9.0	40.0
47	29.838017497661077	35.0	24.0	37.0	9.0	40.0
48	29.245819742303233	34.0	23.0	37.0	9.0	40.0
49	28.83100976386827	33.0	23.0	36.0	8.0	40.0
50	28.145815046399143	33.0	20.0	36.0	8.0	39.0
51	27.750212218742437	33.0	20.0	35.0	8.0	39.0
52	27.339720665951443	33.0	19.0	35.0	8.0	39.0
53	26.762356549160696	33.0	17.0	35.0	8.0	39.0
54	26.201780289484425	32.0	14.0	35.0	8.0	39.0
55	25.571707358481707	31.0	12.0	35.0	8.0	38.0
56	24.715382156286914	30.0	10.0	35.0	8.0	37.0
57	24.024360905514797	28.0	10.0	35.0	8.0	37.0
58	23.34791140635103	27.0	9.0	35.0	8.0	36.0
59	22.54767336013611	26.0	9.0	34.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	7.0
14	18.0
15	50.0
16	175.0
17	519.0
18	1282.0
19	2687.0
20	5234.0
21	8274.0
22	12552.0
23	18227.0
24	24736.0
25	31247.0
26	39025.0
27	44774.0
28	45431.0
29	45049.0
30	48942.0
31	56526.0
32	66102.0
33	77665.0
34	93211.0
35	104940.0
36	109527.0
37	137748.0
38	118318.0
39	15079.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.58306873719915	23.00911727839848	38.3472043115624	8.060609672839975
2	26.705425936561948	21.355255980956304	33.9336866098101	18.00563147267164
3	26.345376521202006	22.30057759620282	32.623258451724304	18.730787430870873
4	25.761820132424496	21.392732907812178	38.16496711061021	14.68047984915311
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	100.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	95.87437734117911	3.6976632458811505	0.27046601429722184	0.1574933986425225
12	96.7591037325213	1.5984135068650505	1.012057636804329	0.6304251238093174
13	91.4779274446696	5.093972265268009	1.6423021489179555	1.785798141144428
14	36.57025614350683	46.365279930067146	7.886499998193883	9.177963928232137
15	21.850854473932312	57.3903596701308	11.81715233151638	8.941633524420507
16	48.96401131351662	28.333911290759545	12.52813027160387	10.17394712411997
17	17.671590141491205	27.23082535932697	25.55122689524883	29.546357603933
18	19.00856821884358	43.10406484682322	23.750347677514206	14.137019256818995
19	42.69543088532241	28.217145829495333	20.470439283766257	8.616984001415997
20	20.571491527505355	34.58786217160279	33.877967901689445	10.962678399202419
21	36.31360692392997	25.864407575576966	22.628478129729768	15.193507370763301
22	43.720673175912175	28.06705751037614	14.940921914339485	13.271347399372194
23	17.3557002857277	26.680320910860907	28.269703832941406	27.69427497046999
24	19.644050470132242	40.399585315546695	13.537388427124986	26.418975787196075
25	18.610319429845	27.05111672211446	36.337086444369795	18.00147740367075
26	16.45869229907852	39.37009865010819	14.102522422942021	30.068686627871273
27	30.979962938480043	42.766140364185425	13.603582613595727	12.650314083738806
28	32.07275400325823	42.00910644169674	12.751456633325747	13.166682921719278
29	33.7758319877762	28.934083955540622	11.136246238761437	26.153837817921737
30	42.64838153859492	31.105849290376646	12.703684839815487	13.542084331212953
31	22.876909517152693	39.55540624988711	13.532782828884867	24.034901404075324
32	21.79938014066039	54.17800005057127	12.346434905738755	11.676184903029581
33	31.93070290459729	45.21433189927647	10.806268670734042	12.048696525392199
34	18.20620076073646	56.09663809389641	11.928409136062015	13.768752009305116
35	27.277694094358772	45.70676968757789	13.64250443401713	13.373031784046209
36	16.999353410129427	46.174915202808876	23.842730559860133	12.983000827201566
37	18.99059735512233	49.684651979323576	17.27975306768965	14.044997597864448
38	21.170490216264444	47.809089825420735	16.63424686729014	14.386173091024682
39	20.548373230456914	48.38587327560984	16.078324067953346	14.98742942597991
40	22.537540140949368	47.38555539902542	15.854004341905165	14.22290011812005
41	28.940315059041964	43.83707741378501	12.846638996954887	14.375968530218142
42	18.333712617894285	45.59316493098827	11.963176887482526	24.10994556363492
43	17.8945552798217	45.1028041771873	14.063600602520617	22.939039940470384
44	17.795941293974433	46.70636511737954	22.646719910994555	12.85097367765147
45	19.097429173123533	54.511680158360335	13.961825911998757	12.429064756517374
46	28.914939115797385	45.46583368552614	13.221227653817952	12.397999544858527
47	30.183104137091497	45.50782590477429	12.523795590907286	11.785274367226924
48	27.8529423451345	46.22097118521007	12.752359691804202	13.173726777851227
49	25.743849268703244	48.40601147967938	12.983994191527866	12.866145060089512
50	19.54679107200266	53.27548340720352	14.303091711006838	12.874633809786987
51	19.252394008026382	53.49384294729389	13.938617309102469	13.315145735577252
52	23.39174315571979	48.647218399274664	12.957263660565605	15.00377478443994
53	18.59668324682033	48.64459952968714	14.113630042227015	18.64508718126551
54	18.98030248846794	50.66808266236088	14.708203744441676	15.643411104729498
55	23.07531146486922	45.49870501414189	16.344636013249676	15.081347507739212
56	20.797165841271216	44.45242146100413	18.50935749195375	16.241055205770905
57	25.361945838164697	41.96557902303522	16.654475377207525	16.01799976159256
58	25.826027590242635	40.843799781098625	16.65041161405448	16.67976101460426
59	22.206478902747826	40.65641514681925	19.059229799484896	18.07787615094803
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	3.0
9	93.0
10	180.0
11	624.5
12	1069.0
13	1947.0
14	3393.0
15	3961.0
16	3409.0
17	2857.0
18	2540.0
19	2254.0
20	2285.0
21	2516.0
22	2747.0
23	3461.5
24	4176.0
25	5364.0
26	9044.0
27	11536.0
28	16424.5
29	21313.0
30	28284.5
31	41848.5
32	48441.0
33	52127.5
34	55814.0
35	55856.5
36	53472.5
37	51046.0
38	49190.0
39	47334.0
40	44205.5
41	41077.0
42	41422.0
43	43139.5
44	44512.0
45	44942.0
46	45372.0
47	45872.5
48	48711.5
49	51050.0
50	52743.5
51	54437.0
52	59655.5
53	69017.0
54	73160.0
55	74903.0
56	76646.0
57	71411.0
58	58039.0
59	49902.0
60	40966.5
61	32031.0
62	27652.5
63	23274.0
64	18491.0
65	12039.0
66	10370.0
67	7773.0
68	5176.0
69	4924.5
70	3583.0
71	2493.0
72	1964.0
73	1435.0
74	1324.0
75	842.5
76	472.0
77	919.0
78	1366.0
79	780.0
80	194.0
81	115.5
82	24.5
83	12.0
84	15.5
85	19.0
86	11.0
87	2.0
88	1.0
89	1.5
90	2.0
91	2.0
92	1.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1107348.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.30897648984956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.42033139103867	53.59651041988048
2	5.9337000507681	7.275781538207212
3	2.1387138655354403	3.933670743018828
4	1.1682227144381911	2.864901557375972
5	0.687140343533343	2.106393558345644
6	0.4712647731415314	1.7335656538217064
7	0.33117127791017925	1.4212640464056032
8	0.24466163024939275	1.1999963309542627
9	0.1968608795828211	1.086240513430286
>10	1.314461478569707	14.310548143663615
>50	0.045855861546340956	1.9060758745718258
>100	0.040919772254222365	5.736551305836563
>500	0.005951965717394891	2.257052936002171
>1k	7.439957146743614E-4	0.5714473784858171
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CACTGCCTAGGGGGTGGGTGGATCACAAGGTCAGAAGTTCAAGATCAGCTTGGTCAACA	1464	0.13220776124578723	No Hit
GGTGGCCTAGGGGGATCACGAGGTCAAGAGGTCAAGACCATCCTGGCCAACGTGGTGAA	1241	0.1120695571762445	No Hit
TTGGGCCTAGAGGGGGCCCCTCGGAGCTGGAAATGCAACTCTCGGGATCCTCGGAGGCT	1234	0.11143741624132612	No Hit
GAATGCCTAGGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCAGG	1168	0.10547723028352424	No Hit
GTTGGCCTAGGGAAGCAGTGGGGGAAAGGTTAACACTTCGGAGGCTGAGGAGGGTAGAT	1157	0.10448386595722392	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.6302445121136264	0.0	0.0	0.0	0.0
10	0.6302445121136264	0.0	0.0	0.0	0.0
11	0.6397266261374022	0.0	0.0	0.0	0.0
12	0.6883111722782721	0.0	0.0	0.0	0.0
13	0.8004710353023621	0.0	0.0	0.0	0.0
14	1.4345986988733443	0.0	0.0	0.0	0.0
15	1.8373627802641987	0.0	0.0	0.0	0.0
16	2.081278875294849	0.0	0.0	0.0	0.0
17	2.3480423498304055	0.0	0.0	0.0	0.0
18	2.993097020990691	0.0	0.0	0.0	0.0
19	3.217055523647489	0.0	0.0	0.0	0.0
20	3.360912739265344	0.0	0.0	0.0	0.0
21	3.519218890538476	0.0	0.0	0.0	0.0
22	3.672558220180106	0.0	0.0	0.0	0.0
23	3.808649132883249	0.0	0.0	0.0	0.0
24	4.01635258292786	0.0	0.0	0.0	0.0
25	4.197957642945126	0.0	0.0	0.0	0.0
26	4.473661396417387	0.0	0.0	0.0	0.0
27	4.6955428645737385	0.0	0.0	0.0	0.0
28	4.9699823361761615	0.0	0.0	0.0	0.0
29	5.147794550583917	0.0	0.0	0.0	0.0
30	5.409952426879355	0.0	0.0	0.0	0.0
31	5.6904423902874255	0.0	0.0	0.0	0.0
32	6.283119669697331	0.0	0.0	0.0	0.0
33	6.506536337267056	0.0	0.0	0.0	0.0
34	6.812582855615398	0.0	0.0	0.0	0.0
35	6.994368527328356	0.0	0.0	0.0	0.0
36	7.203787788482031	0.0	0.0	0.0	0.0
37	7.404266770699003	0.0	0.0	0.0	0.0
38	7.598424343566792	0.0	0.0	0.0	0.0
39	7.823466516397736	0.0	0.0	0.0	0.0
40	8.086617757019473	0.0	0.0	0.0	0.0
41	8.358709276577914	0.0	0.0	0.0	0.0
42	8.651932364532197	0.0	0.0	0.0	0.0
43	8.95436664896672	0.0	0.0	0.0	0.0
44	9.279558007058306	0.0	0.0	0.0	0.0
45	9.645477302528203	0.0	0.0	0.0	0.0
46	9.936803967677731	0.0	0.0	0.0	0.0
47	10.189208812405855	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGT	15	0.006696201	53.000004	52
CTCGTCG	15	0.006696201	53.000004	42
CAAGTAA	15	0.006696201	53.000004	26
CTATATT	30	7.0102396E-7	53.000004	48
CTCCCGA	15	0.006696201	53.000004	18
ACCGACT	30	7.0102396E-7	53.000004	46
TAAACAC	75	0.0	53.000004	41
CAACATA	15	0.006696201	53.000004	26
TACATGA	15	0.006696201	53.000004	43
CTGCGAC	30	7.0102396E-7	53.000004	48
CTAACTA	15	0.006696201	53.000004	28
ACGATGC	15	0.006696201	53.000004	31
CCACTAT	90	0.0	53.000004	17
CACTCAC	60	0.0	53.000004	48
TCTAATA	15	0.006696201	53.000004	53
CGTACTA	15	0.006696201	53.000004	49
CGAGCTA	45	7.6397555E-11	53.000004	21
TACTCGA	15	0.006696201	53.000004	16
CCCCACG	15	0.006696201	53.000004	28
TCCCGGA	30	7.0102396E-7	53.000004	32
>>END_MODULE