FastQCFastQC Report
Fri 17 Jun 2016
SRR1527948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4099716
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA351.0204349E-746.00000434
CTATACG750.046.00000431
GCGTTAG351.0204349E-746.00000444
CGAATTA351.0204349E-746.00000444
ATACGTC750.046.00000433
CGATACC351.0204349E-746.00000434
ATACCGT750.046.00000436
ACGAATT351.0204349E-746.00000443
AGCGTTA206.3133606E-446.027
TAATAGC551.8189894E-1246.016
ACTATAC551.8189894E-1246.042
CGAACTT551.8189894E-1246.043
CGTATTA206.3133606E-446.041
CTACTAT301.8625815E-646.022
CGTATAA253.4187025E-546.034
CCGATTA206.3133606E-446.016
CTATATA600.046.038
TAGGTCG206.3133606E-446.025
TTAGACG600.046.044
TAGCGAT405.6170393E-946.042
Produced by FastQC (version 0.11.5)