##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527948_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4099716
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79449381371783	33.0	31.0	34.0	30.0	34.0
2	32.32606039052461	34.0	31.0	34.0	30.0	34.0
3	32.44608138710096	34.0	31.0	34.0	30.0	34.0
4	35.99218262923578	37.0	35.0	37.0	35.0	37.0
5	35.97590833121124	37.0	35.0	37.0	35.0	37.0
6	35.82060074405154	37.0	35.0	37.0	33.0	37.0
7	35.58736166114921	37.0	35.0	37.0	33.0	37.0
8	35.88484738942893	37.0	35.0	37.0	35.0	37.0
9	37.448355934898906	39.0	37.0	39.0	34.0	39.0
10	37.82793710588734	39.0	38.0	39.0	35.0	39.0
11	38.105228264591986	39.0	39.0	39.0	37.0	39.0
12	38.11255657708973	39.0	39.0	39.0	37.0	39.0
13	38.12889770901204	39.0	39.0	39.0	37.0	39.0
14	38.78879073574853	40.0	39.0	40.0	36.0	40.0
15	38.15041700449495	40.0	38.0	40.0	34.0	40.0
16	37.52278304155703	40.0	36.0	40.0	33.0	40.0
17	37.47473166433968	40.0	37.0	40.0	33.0	40.0
18	37.539907886302366	40.0	37.0	40.0	33.0	40.0
19	37.668055543359586	40.0	37.0	40.0	33.0	40.0
20	37.70814954011448	40.0	37.0	40.0	33.0	40.0
21	37.55308196958033	40.0	37.0	40.0	33.0	40.0
22	37.590700429005324	40.0	37.0	40.0	33.0	40.0
23	37.53846705479111	40.0	37.0	40.0	33.0	40.0
24	37.49530699199652	40.0	37.0	40.0	33.0	40.0
25	37.60999688758929	40.0	37.0	40.0	33.0	40.0
26	37.6008825977214	40.0	37.0	40.0	33.0	40.0
27	37.560708839343995	40.0	37.0	40.0	33.0	40.0
28	37.51390706087934	40.0	37.0	40.0	33.0	40.0
29	37.51041120897155	40.0	37.0	40.0	33.0	40.0
30	37.45773560900316	40.0	36.0	40.0	33.0	40.0
31	37.410771868100134	40.0	36.0	40.0	33.0	40.0
32	37.31673608610938	40.0	36.0	40.0	33.0	40.0
33	37.20957939525567	40.0	35.0	40.0	33.0	40.0
34	37.08822025720806	40.0	35.0	40.0	32.0	40.0
35	36.97847509437239	39.0	35.0	40.0	32.0	40.0
36	36.85640273618953	39.0	35.0	40.0	32.0	40.0
37	36.69713780174041	39.0	35.0	40.0	32.0	40.0
38	36.49669733220545	39.0	35.0	40.0	31.0	40.0
39	36.34141194170523	39.0	35.0	40.0	31.0	40.0
40	36.1563518058324	38.0	35.0	40.0	31.0	40.0
41	35.94542378057407	38.0	35.0	40.0	30.0	40.0
42	35.763729487603534	38.0	35.0	40.0	30.0	40.0
43	35.50694584698062	37.0	35.0	40.0	30.0	40.0
44	35.283560617369595	37.0	34.0	40.0	29.0	40.0
45	35.08173346641572	36.0	34.0	40.0	28.0	40.0
46	34.88410099626413	36.0	34.0	40.0	28.0	40.0
47	34.690877855929536	36.0	34.0	39.0	28.0	40.0
48	34.48314517395839	35.0	34.0	39.0	27.0	40.0
49	34.31089397411918	35.0	34.0	39.0	27.0	40.0
50	34.118417226949376	35.0	33.0	39.0	27.0	40.0
51	33.91477677965986	35.0	33.0	38.0	26.0	40.0
52	33.23081696390677	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	6.0
14	11.0
15	28.0
16	62.0
17	223.0
18	517.0
19	1144.0
20	2268.0
21	3882.0
22	6402.0
23	9663.0
24	14452.0
25	20490.0
26	28118.0
27	37639.0
28	50411.0
29	64473.0
30	81818.0
31	100483.0
32	121653.0
33	152145.0
34	223818.0
35	370996.0
36	390617.0
37	769281.0
38	1191425.0
39	457685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.12372466775747	20.92618122816312	34.48053474923629	15.469559354843115
2	28.867926461247563	20.648479065379163	32.59691646933592	17.886678004037353
3	20.89700847570905	21.58337309218492	35.49658073876337	22.023037693342662
4	22.61432255307441	21.323745352117072	35.674739420974525	20.387192673833994
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.46788997091505	0.41244320338286844	0.08517663174717469	0.034490193954898336
12	99.44052222153925	0.402954741255248	0.08107878692085013	0.07544425028465386
13	98.14170054706229	0.986897629006497	0.27453121143025516	0.5968706125009634
14	68.03639569179913	20.129857775514207	4.35371620863494	7.4800303240517145
15	35.48392132528204	27.00450470227694	15.569395538617798	21.94217843382322
16	28.881707903669422	18.84008550836204	27.76111808720409	24.517088500764444
17	23.358447268054665	17.66556512695026	30.285951514690286	28.690036090304794
18	24.231873622465557	18.671659207613406	30.30031836351591	26.796148806405128
19	25.17623171946545	17.668687294436978	28.167097428212102	28.98798355788547
20	28.365574591020454	17.872433114879176	24.692076231621897	29.069916062478473
21	32.11690761018568	18.90855366566855	19.455250070980526	29.519288653165244
22	31.112130693930993	20.12263776320116	20.938523546509074	27.826707996358774
23	30.234972373696127	18.761763009925566	19.98843334513903	31.014831271239274
24	30.22736209044724	22.412552479244905	20.291161631683753	27.068923798624102
25	32.91730451572743	19.229161239461465	21.529344959504513	26.32418928530659
26	32.33284939737289	19.876718289754706	19.57384365160904	28.216588661263366
27	31.984971641938127	21.804632320872958	18.43878941858412	27.7716066186048
28	31.89533128636228	21.298011862285097	18.914090634570783	27.892566216781844
29	30.328832533765755	20.308114025459325	19.722414918496796	29.640638522278127
30	31.50498717472137	19.2623342690079	19.84608202129123	29.386596534979496
31	30.6574406617434	20.05951143932897	19.470690164879713	29.812357734047918
32	32.10944367853773	21.41850801372583	18.601971453632398	27.870076854104042
33	32.6270648991296	20.618062324317098	18.916798139188177	27.838074637365125
34	31.214893909724477	21.657256258726214	18.97402161515578	28.153828216393528
35	32.76546472975201	22.90407433100244	18.02739506834132	26.30306587090423
36	33.782193693416815	21.5533466220587	18.282900571649353	26.381559112875134
37	33.366457579012796	22.03145291039672	18.690733699602607	25.911355810987885
38	31.15557272747673	23.50031075323266	20.551008899153015	24.79310762013759
39	33.06016807017852	23.01142323029205	20.507664433341237	23.420744266188194
40	34.419237820375855	22.404649492794135	18.645315919444176	24.530796767385837
41	33.82046463706266	22.737062762396224	18.58938521595154	24.853087384589568
42	34.28613103932077	22.964419974456767	18.339392289612256	24.410056696610205
43	33.38828835948636	23.113259552612913	18.417519652580815	25.08093243531991
44	31.444909842535434	22.899269120104904	18.814327626596576	26.841493410763086
45	29.73374253240956	22.4108450439006	20.611622853875733	27.243789569814105
46	29.869337290680626	21.288108737288145	21.157075270579718	27.685478701451515
47	31.132937013197985	18.932994383025555	22.466190340989474	27.467878262786982
48	30.542042424402084	19.652385677446926	21.916810822993593	27.888761075157404
49	29.412037321609596	19.764320260232658	21.74392079841628	29.079721619741466
50	30.6471716577441	20.341067527604352	21.57115273350642	27.440608081145136
51	30.080620218571237	21.605057521057557	21.16946637279265	27.144855887578558
52	31.265043724979975	20.830125794079397	21.72704158044118	26.17778890049945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	4.0
8	6.0
9	20.5
10	35.0
11	197.0
12	359.0
13	528.0
14	1000.0
15	1303.0
16	1480.0
17	1657.0
18	2201.0
19	2745.0
20	3600.5
21	4456.0
22	6164.0
23	7872.0
24	10515.0
25	13158.0
26	15954.0
27	18750.0
28	22007.0
29	25264.0
30	26091.0
31	26918.0
32	26497.5
33	26077.0
34	26923.5
35	27770.0
36	28225.5
37	28681.0
38	30605.5
39	33461.5
40	34393.0
41	38278.5
42	42164.0
43	48308.0
44	54452.0
45	65098.5
46	75745.0
47	87457.5
48	99170.0
49	115942.5
50	132715.0
51	149028.5
52	165342.0
53	197774.0
54	230206.0
55	258751.0
56	287296.0
57	324464.5
58	361633.0
59	390083.0
60	418533.0
61	426940.0
62	435347.0
63	414670.5
64	354529.5
65	315065.0
66	288107.0
67	261149.0
68	231469.5
69	201790.0
70	175752.5
71	149715.0
72	127594.5
73	105474.0
74	84967.5
75	64461.0
76	47829.5
77	31198.0
78	23556.0
79	15914.0
80	9998.0
81	4082.0
82	2709.5
83	1337.0
84	755.0
85	173.0
86	121.0
87	69.0
88	41.0
89	8.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4099716.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.26839389411703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33884800274488	7.075385127125687
2	7.861182183958126	1.4572106590867817
3	3.643479770452887	1.0130761697341337
4	2.169769505494189	0.8044111374545467
5	1.3909055312790368	0.6445730166700114
6	0.9423324245912458	0.5240344854186
7	0.6657904820147801	0.43195659067869163
8	0.49802661386678765	0.3692725461656572
9	0.36221063708590984	0.3021399771436158
>10	2.5178140056553664	4.6777765757323095
>50	0.5925268183834441	4.078149816078464
>100	2.737658502220353	60.72248310797397
>500	0.25775537753268757	15.42325905578664
>1k	0.021700144720411072	2.4762717349509207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004585683496125098	0.0	0.0	0.0	0.0
12	0.008122513852179028	0.0	0.0	0.0	0.0
13	0.014244889158175835	0.0	0.0	0.0	0.0
14	0.04073452892834528	0.0	0.0	0.0	0.0
15	0.0714195812587994	0.0	0.0	0.0	0.0
16	0.09322596979888363	0.0	0.0	0.0	0.0
17	0.10520240914248695	0.0	0.0	0.0	0.0
18	0.11981317730301319	0.0	0.0	0.0	0.0
19	0.13688753074603216	0.0	0.0	0.0	0.0
20	0.1721094827056313	0.0	0.0	0.0	0.0
21	0.21708820806124132	0.0	0.0	0.0	0.0
22	0.24313879302859026	0.0	0.0	0.0	0.0
23	0.2649451815686745	0.0	0.0	0.0	0.0
24	0.2882638699851404	0.0	0.0	0.0	0.0
25	0.3133143856793983	0.0	0.0	0.0	0.0
26	0.34541417015227394	0.0	0.0	0.0	0.0
27	0.3766602369529987	0.0	0.0	0.0	0.0
28	0.41097968737346685	0.0	0.0	0.0	7.317580047008134E-5
29	0.45110441796456147	0.0	0.0	0.0	7.317580047008134E-5
30	0.4978393625314534	0.0	0.0	0.0	7.317580047008134E-5
31	0.5385738914597987	0.0	0.0	0.0	7.317580047008134E-5
32	0.5745763852910787	0.0	0.0	0.0	7.317580047008134E-5
33	0.6063102907616039	0.0	0.0	0.0	7.317580047008134E-5
34	0.6380929800991093	0.0	0.0	0.0	7.317580047008134E-5
35	0.6686316808286232	0.0	0.0	0.0	7.317580047008134E-5
36	0.6970970672114849	0.0	0.0	0.0	7.317580047008134E-5
37	0.7253673181264263	0.0	0.0	0.0	7.317580047008134E-5
38	0.7531253384380772	0.0	0.0	0.0	7.317580047008134E-5
39	0.7810784942176483	0.0	0.0	0.0	7.317580047008134E-5
40	0.812324561018373	0.0	0.0	0.0	7.317580047008134E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	35	1.0204349E-7	46.000004	34
CTATACG	75	0.0	46.000004	31
GCGTTAG	35	1.0204349E-7	46.000004	44
CGAATTA	35	1.0204349E-7	46.000004	44
ATACGTC	75	0.0	46.000004	33
CGATACC	35	1.0204349E-7	46.000004	34
ATACCGT	75	0.0	46.000004	36
ACGAATT	35	1.0204349E-7	46.000004	43
AGCGTTA	20	6.3133606E-4	46.0	27
TAATAGC	55	1.8189894E-12	46.0	16
ACTATAC	55	1.8189894E-12	46.0	42
CGAACTT	55	1.8189894E-12	46.0	43
CGTATTA	20	6.3133606E-4	46.0	41
CTACTAT	30	1.8625815E-6	46.0	22
CGTATAA	25	3.4187025E-5	46.0	34
CCGATTA	20	6.3133606E-4	46.0	16
CTATATA	60	0.0	46.0	38
TAGGTCG	20	6.3133606E-4	46.0	25
TTAGACG	60	0.0	46.0	44
TAGCGAT	40	5.6170393E-9	46.0	42
>>END_MODULE