FastQCFastQC Report
Fri 17 Jun 2016
SRR1527947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4065876
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA206.3133525E-446.00000441
CGTATAC351.0204349E-746.00000442
CTATATG206.3133525E-446.00000439
ATAGCGC351.0204349E-746.00000432
AAGGTAC351.0204349E-746.00000445
TCGATCA206.3133525E-446.00000445
CATTATC206.3133525E-446.00000429
AATATAG351.0204349E-746.00000415
GTTACAT206.3133525E-446.00000419
TACGCAT206.3133525E-446.00000424
ACTAATA206.3133525E-446.00000436
ACGCTAA551.8189894E-1246.00000419
TTACGCC700.046.00000426
ATTATCG206.3133525E-446.00000427
CGCAATT206.3133525E-446.00000445
GACCTAA206.3133525E-446.00000413
GCGTATT206.3133525E-446.00000437
TACGTAC405.6170393E-946.00000415
TACCGAT206.3133525E-446.00000419
CTACTAT301.8625778E-646.022
Produced by FastQC (version 0.11.5)