FastQCFastQC Report
Fri 17 Jun 2016
SRR1527946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4065015
Sequences flagged as poor quality0
Sequence length59
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAT251.4794021E-553.00000436
CTACGTT251.4794021E-553.00000417
TATAGGT251.4794021E-553.00000423
TACTAAC251.4794021E-553.00000424
ATACGTC457.6397555E-1153.00000433
CCGTTAT251.4794021E-553.00000432
ACGAATT503.6379788E-1253.00000443
CGTATAA150.006697482453.034
GACGTTA203.1391936E-453.050
GACGTAA203.1391936E-453.029
ACTTATA203.1391936E-453.044
TAACGAC150.006697482453.043
TCGATAA150.006697482453.028
CGCTAAT550.053.015
CGAATCA150.006697482453.021
ACGTAAG2650.053.027
TATCGAT307.0149144E-753.025
CGATACC353.3449396E-853.034
TAGTAAT353.3449396E-853.038
TACCGAT353.3449396E-853.019