##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4065015
Sequences flagged as poor quality	0
Sequence length	59
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.353706689889215	31.0	31.0	34.0	30.0	34.0
2	31.875260976896765	31.0	31.0	34.0	30.0	34.0
3	32.101098765933216	33.0	31.0	34.0	30.0	34.0
4	35.775675366511564	37.0	35.0	37.0	35.0	37.0
5	35.65529130888816	37.0	35.0	37.0	33.0	37.0
6	35.510849775462084	37.0	35.0	37.0	32.0	37.0
7	35.15220534241571	37.0	35.0	37.0	32.0	37.0
8	35.58444630585619	37.0	35.0	37.0	33.0	37.0
9	37.04670979073878	39.0	37.0	39.0	33.0	39.0
10	37.47520292053043	39.0	37.0	39.0	35.0	39.0
11	37.78077965271961	39.0	37.0	39.0	35.0	39.0
12	37.84725148615688	39.0	37.0	39.0	35.0	39.0
13	37.88166218328838	39.0	38.0	39.0	35.0	39.0
14	38.335806878941405	40.0	38.0	40.0	35.0	40.0
15	37.6283413468339	40.0	37.0	40.0	33.0	40.0
16	37.02259647258374	39.0	36.0	40.0	32.0	40.0
17	36.90268596794846	39.0	36.0	40.0	31.0	40.0
18	36.94629761513795	39.0	36.0	40.0	31.0	40.0
19	37.09293225240251	39.0	36.0	40.0	32.0	40.0
20	37.22002551036097	39.0	36.0	40.0	32.0	40.0
21	37.21472048688627	40.0	36.0	40.0	32.0	40.0
22	37.16270198264951	39.0	36.0	40.0	32.0	40.0
23	37.10248104865542	39.0	36.0	40.0	32.0	40.0
24	37.04646477319272	39.0	36.0	40.0	31.0	40.0
25	36.97357205323966	39.0	36.0	40.0	31.0	40.0
26	36.82981145211026	39.0	35.0	40.0	31.0	40.0
27	36.69083312115699	39.0	35.0	40.0	31.0	40.0
28	36.656762398170734	39.0	35.0	40.0	31.0	40.0
29	36.61438494076898	39.0	35.0	40.0	30.0	40.0
30	36.546607085090706	39.0	35.0	40.0	30.0	40.0
31	36.53320393651684	39.0	35.0	40.0	30.0	40.0
32	36.370796417725394	39.0	35.0	40.0	30.0	40.0
33	36.17935136770713	39.0	35.0	40.0	30.0	40.0
34	36.06499238010192	38.0	35.0	40.0	30.0	40.0
35	35.916430320675325	38.0	35.0	40.0	29.0	40.0
36	35.683968447840904	38.0	35.0	40.0	29.0	40.0
37	35.508694063859544	38.0	34.0	40.0	28.0	40.0
38	35.29052168319182	38.0	34.0	40.0	27.0	40.0
39	35.1059420444943	37.0	34.0	40.0	27.0	40.0
40	34.86746346569447	37.0	33.0	40.0	26.0	40.0
41	34.667594338520274	37.0	33.0	40.0	26.0	40.0
42	34.5211370683749	37.0	33.0	40.0	26.0	40.0
43	34.23067098153389	36.0	33.0	40.0	25.0	40.0
44	33.99430654007427	36.0	33.0	39.0	24.0	40.0
45	33.757427955370396	35.0	33.0	39.0	23.0	40.0
46	33.50096863111206	35.0	33.0	39.0	23.0	40.0
47	33.32195699154862	35.0	32.0	39.0	23.0	40.0
48	33.0635112047557	35.0	32.0	38.0	23.0	40.0
49	32.940163074429	35.0	32.0	38.0	22.0	40.0
50	32.698965194470375	35.0	32.0	38.0	22.0	40.0
51	32.46983811867853	35.0	31.0	37.0	21.0	40.0
52	32.2619392548367	35.0	31.0	37.0	20.0	40.0
53	31.996218956141615	35.0	31.0	37.0	20.0	39.0
54	31.80358965465072	35.0	31.0	36.0	20.0	39.0
55	31.610402913642385	34.0	30.0	36.0	20.0	39.0
56	31.372579190974694	34.0	30.0	36.0	19.0	39.0
57	31.19023595238886	34.0	30.0	35.0	18.0	39.0
58	31.00046789495242	34.0	30.0	35.0	18.0	38.0
59	30.45255700163468	34.0	29.0	35.0	17.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	8.0
14	28.0
15	96.0
16	372.0
17	1055.0
18	2431.0
19	4893.0
20	8497.0
21	12858.0
22	18967.0
23	26340.0
24	35528.0
25	46178.0
26	58974.0
27	73097.0
28	88429.0
29	103469.0
30	121361.0
31	142852.0
32	170767.0
33	215555.0
34	303522.0
35	406848.0
36	529289.0
37	783130.0
38	782450.0
39	128015.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.842771797890045	21.38528394114167	34.06285093658941	15.709093324378875
2	27.903734672565783	21.369835043659123	32.581158987113206	18.145271296661882
3	21.05419045194175	21.83020234857682	35.52872498625466	21.586882213226765
4	22.342771182886164	21.552195994356723	35.686879384208915	20.4181534385482
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.4450696983898	0.4190390441363685	0.09589140507476603	0.03999985239906864
12	99.43026040494316	0.405189156743579	0.08462453398080942	0.07992590433245635
13	98.09784219738427	1.017757622050595	0.28120437439960244	0.6031958061655369
14	67.71869230494845	20.377390981337093	4.418212478920742	7.485704234793722
15	35.43042276596765	27.115078296144045	15.569143041292591	21.88535589659571
16	28.892833113776952	18.992648243610414	27.70016838806253	24.414350254550108
17	23.257035951896857	17.816933024847387	30.228547742136254	28.697483281119503
18	24.189234234068017	18.924358212700323	30.12357887978273	26.76282867344893
19	25.18765613411021	17.772775746214958	28.09052365120424	28.949044468470596
20	28.27123639150163	18.07100342803163	24.67550550243972	28.98225467802702
21	31.820595987960683	19.156583678043006	19.47466368512785	29.548156648868456
22	31.02465796559176	20.34974040686197	20.942259745658994	27.683341881887273
23	30.15553448142258	18.985587999060275	19.972816828474187	30.88606069104296
24	30.210343627268287	22.641982870911914	20.115842130963845	27.03183137085595
25	32.81820608288039	19.435451037696048	21.56917010146334	26.177172777960227
26	32.243202054604964	20.034883020111856	19.564552652327237	28.157362272955943
27	31.876167738618435	21.95846755793029	18.542957406061234	27.622407297390044
28	31.8919118379637	21.509588525503595	18.865219439534663	27.733280196998045
29	30.26030654253428	20.444721606193337	19.734219922927714	29.56075192834467
30	31.487017883082842	19.510383110517427	19.805412772154593	29.197186234245137
31	30.651842613126888	20.26026964230144	19.40511904630118	29.682768698270483
32	31.997692505439705	21.611211766746248	18.620078892697816	27.77101683511623
33	32.54268434433821	20.845285933754244	18.90204587190945	27.709983849998093
34	31.1748665134077	21.950841509809926	18.889327591657104	27.98496438512527
35	32.63896935189661	23.131206157910857	18.076194060784523	26.153630429408008
36	33.69805523472853	21.777828618098578	18.35004298877126	26.17407315840163
37	33.30747857018978	22.224370635778712	18.674519036215116	25.793631757816392
38	31.059294984151347	23.72589031036786	20.500244156540628	24.714570548940166
39	32.961132984749135	23.28655613816923	20.320589222918	23.43172165416364
40	34.30641707349174	22.678095898785124	18.62937775137361	24.386109276349533
41	33.778596142941666	22.997159912079045	18.52320348141397	24.701040463565324
42	34.196626580713726	23.172583619002634	18.28763239496041	24.343157405323225
43	33.25139021627226	23.37078706966641	18.418677421854532	24.959145292206795
44	31.35621885774099	23.083727858322785	18.742513865262488	26.817539418673732
45	29.635216598216736	22.801834679576828	20.510256419718008	27.052692302488428
46	29.89838905883496	21.529317849995632	21.019184431053763	27.553108660115644
47	31.058532379339315	19.158158087977537	22.321664249701413	27.461645282981735
48	30.424094376035516	19.876605621381472	21.88046046570554	27.818839536877476
49	29.31866180075596	20.06142658760177	21.704766157074452	28.91514545456782
50	30.594745652845067	20.63581561199651	21.40946589372979	27.359972841428633
51	29.989040630846382	21.863978361703463	21.08981147671042	27.05716953073974
52	31.17479271294202	20.981841395419202	21.710497993242335	26.13286789839644
53	28.550398953017393	21.46466864205913	22.131677250883453	27.853255154040024
54	28.552760567919183	21.329884391570513	22.15827001868382	27.959085021826485
55	29.09278317546184	21.511704138853116	21.48892439511293	27.906588290572117
56	29.88097214893426	21.887545310410907	20.875347323441613	27.35613521721322
57	29.837921877287044	21.08164422517506	20.790550588374213	28.28988330916368
58	30.154255273350778	21.391409379793185	20.666393604943647	27.78794174191239
59	29.534651163648846	20.69121516156767	21.319453925754274	28.454679749029214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	2.5
7	4.0
8	19.0
9	134.5
10	235.0
11	347.5
12	460.0
13	634.5
14	815.0
15	821.0
16	1037.0
17	1253.0
18	1402.5
19	1825.0
20	2098.0
21	2675.5
22	3253.0
23	4251.0
24	5249.0
25	7336.5
26	12340.5
27	15257.0
28	17781.5
29	20306.0
30	22367.0
31	26104.0
32	27780.0
33	29126.5
34	30473.0
35	32146.0
36	33426.0
37	33033.0
38	33108.0
39	33183.0
40	35626.5
41	38070.0
42	41393.5
43	51929.0
44	59141.0
45	66232.0
46	73323.0
47	76505.5
48	89336.0
49	98984.0
50	113452.5
51	127921.0
52	146615.5
53	179276.5
54	193243.0
55	222189.5
56	251136.0
57	289297.0
58	340976.0
59	354494.0
60	361803.5
61	369113.0
62	364761.0
63	360409.0
64	343848.0
65	289214.5
66	251142.0
67	227737.0
68	204332.0
69	177843.5
70	138695.5
71	126036.0
72	114736.5
73	103437.0
74	80207.5
75	42333.0
76	27688.0
77	22897.5
78	18107.0
79	12469.5
80	6832.0
81	5303.0
82	2378.5
83	983.0
84	651.0
85	319.0
86	258.5
87	122.5
88	47.0
89	29.0
90	11.0
91	9.5
92	5.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	4065015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.592632274975836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.0049055848715	11.146625263592703
2	6.69046658555865	1.818821040910238
3	2.8641677019518785	1.1679473503948343
4	1.676297180746416	0.9114116464585894
5	1.0096797089688525	0.6862102499759117
6	0.6894239120851732	0.5622651431129418
7	0.47049219825191213	0.44766592073582895
8	0.3210195346273509	0.3490800389818762
9	0.2436095504090529	0.29801655336442007
>10	1.5894462605356388	4.385556726836947
>50	0.4229253694967323	4.269439361549096
>100	1.8535783921726339	58.58446567978879
>500	0.15243181754988322	13.423266634556985
>1k	0.011556202774539883	1.949228389740858
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004674029493126101	0.0	0.0	0.0	0.0
12	0.008831455726485634	0.0	0.0	0.0	0.0
13	0.013948288013697366	0.0	0.0	0.0	0.0
14	0.036432829891156614	0.0	0.0	0.0	0.0
15	0.06514121104104167	0.0	0.0	0.0	0.0
16	0.08440313258376661	0.0	0.0	0.0	0.0
17	0.09606360616135488	0.0	0.0	0.0	0.0
18	0.10986429324369036	0.0	0.0	0.0	0.0
19	0.1260265952278159	0.0	0.0	0.0	0.0
20	0.15881860214537954	0.0	0.0	0.0	0.0
21	0.20002386215057016	0.0	0.0	0.0	0.0
22	0.22543582250003996	0.0	0.0	0.0	0.0
23	0.24432874171436023	0.0	0.0	0.0	0.0
24	0.26585387753796724	0.0	0.0	0.0	0.0
25	0.2895684271767755	0.0	0.0	0.0	0.0
26	0.3170222004100846	0.0	0.0	0.0	0.0
27	0.3456567810942887	0.0	0.0	0.0	0.0
28	0.3781535861491286	0.0	0.0	0.0	1.476009313618769E-4
29	0.4150292188343708	0.0	0.0	0.0	1.476009313618769E-4
30	0.4558654765111568	0.0	0.0	0.0	1.476009313618769E-4
31	0.495201124719097	0.0	0.0	0.0	1.476009313618769E-4
32	0.5295183412607334	0.0	0.0	0.0	1.476009313618769E-4
33	0.5605145368467275	0.0	0.0	0.0	1.476009313618769E-4
34	0.5889277161338888	0.0	0.0	0.0	1.476009313618769E-4
35	0.6160862875044741	0.0	0.0	0.0	1.476009313618769E-4
36	0.642654455149612	0.0	0.0	0.0	1.476009313618769E-4
37	0.6699360272963323	0.0	0.0	0.0	1.476009313618769E-4
38	0.6962335932339733	0.0	0.0	0.0	1.476009313618769E-4
39	0.7237857670881903	0.0	0.0	0.0	1.476009313618769E-4
40	0.7531829525844308	0.0	0.0	0.0	1.476009313618769E-4
41	0.780513725041605	0.0	0.0	0.0	1.476009313618769E-4
42	0.8055566830626701	0.0	0.0	0.0	1.476009313618769E-4
43	0.831977249776446	0.0	0.0	0.0	1.476009313618769E-4
44	0.8571432085736461	0.0	0.0	0.0	1.476009313618769E-4
45	0.8823583676813	0.0	0.0	0.0	1.476009313618769E-4
46	0.9070815236844144	0.0	0.0	0.0	1.476009313618769E-4
47	0.9317554793770749	0.0	0.0	0.0	1.7220108658885637E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATTAT	25	1.4794021E-5	53.000004	36
CTACGTT	25	1.4794021E-5	53.000004	17
TATAGGT	25	1.4794021E-5	53.000004	23
TACTAAC	25	1.4794021E-5	53.000004	24
ATACGTC	45	7.6397555E-11	53.000004	33
CCGTTAT	25	1.4794021E-5	53.000004	32
ACGAATT	50	3.6379788E-12	53.000004	43
CGTATAA	15	0.0066974824	53.0	34
GACGTTA	20	3.1391936E-4	53.0	50
GACGTAA	20	3.1391936E-4	53.0	29
ACTTATA	20	3.1391936E-4	53.0	44
TAACGAC	15	0.0066974824	53.0	43
TCGATAA	15	0.0066974824	53.0	28
CGCTAAT	55	0.0	53.0	15
CGAATCA	15	0.0066974824	53.0	21
ACGTAAG	265	0.0	53.0	27
TATCGAT	30	7.0149144E-7	53.0	25
CGATACC	35	3.3449396E-8	53.0	34
TAGTAAT	35	3.3449396E-8	53.0	38
TACCGAT	35	3.3449396E-8	53.0	19
>>END_MODULE