FastQCFastQC Report
Fri 17 Jun 2016
SRR1527945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226704
Sequences flagged as poor quality0
Sequence length52
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA1050.046.00000423
CATATAG1050.046.00000442
ATCCGAT1050.046.00000425
TCGGACA1050.046.00000417
CCATATA1050.046.00000441
AACGACC551.8189894E-1246.00000435
TCCCGAT1050.046.00000440
ATTGATC551.8189894E-1246.00000414
ACCTTAT551.8189894E-1246.00000445
TCACGAA301.862194E-646.038
CGGAATA750.046.013
ACTATTG405.6152203E-946.018
AACCGTC800.046.034
TAATAGC301.862194E-646.041
TCGCATA253.418195E-546.031
CATATGG301.862194E-646.044
ATTAGCA405.6152203E-946.026
CTCAAAC253.418195E-546.015
TCACATA405.6152203E-946.029
TAGGTTA206.3127367E-446.022
Produced by FastQC (version 0.11.5)