##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2226704
Sequences flagged as poor quality	0
Sequence length	52
%GC	61
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.253551437460928	31.0	31.0	33.0	30.0	34.0
2	31.857983818235383	31.0	31.0	34.0	30.0	34.0
3	32.023577449000854	31.0	31.0	34.0	30.0	34.0
4	35.816692744073755	37.0	35.0	37.0	35.0	37.0
5	35.828687602842585	37.0	35.0	37.0	35.0	37.0
6	35.784230863195106	37.0	35.0	37.0	35.0	37.0
7	34.95794591467927	35.0	35.0	36.0	33.0	37.0
8	35.40844809188828	35.0	35.0	37.0	35.0	37.0
9	37.219372220106486	39.0	37.0	39.0	35.0	39.0
10	37.262058630154705	39.0	37.0	39.0	35.0	39.0
11	38.013780008478896	39.0	37.0	39.0	35.0	39.0
12	37.985344257701065	39.0	37.0	39.0	35.0	39.0
13	37.60024143307777	39.0	37.0	39.0	35.0	39.0
14	37.316631218159216	40.0	37.0	40.0	31.0	40.0
15	34.50564511493221	37.0	32.0	39.0	25.0	40.0
16	33.510480063807314	36.0	31.0	39.0	22.0	40.0
17	33.216417179831716	35.0	31.0	39.0	22.0	40.0
18	33.1811794472907	35.0	31.0	39.0	22.0	40.0
19	33.40514365627403	35.0	31.0	39.0	23.0	40.0
20	33.36696525447478	35.0	31.0	39.0	23.0	40.0
21	32.95098495354569	35.0	30.0	38.0	23.0	40.0
22	32.8309115176512	35.0	30.0	38.0	23.0	40.0
23	32.47520236187657	34.0	30.0	38.0	22.0	40.0
24	32.258846707959385	34.0	29.0	37.0	22.0	40.0
25	32.949768806271514	35.0	30.0	38.0	23.0	40.0
26	32.96413353548563	35.0	31.0	38.0	23.0	40.0
27	32.99869897390942	35.0	31.0	37.0	24.0	40.0
28	32.95461273703195	35.0	31.0	37.0	24.0	40.0
29	32.80405478231503	35.0	31.0	37.0	23.0	40.0
30	32.677851658774586	35.0	31.0	37.0	23.0	40.0
31	32.45714832326165	34.0	31.0	36.0	23.0	39.0
32	32.176329678304796	34.0	31.0	36.0	22.0	39.0
33	31.94446859573612	34.0	30.0	36.0	22.0	39.0
34	31.76128888258161	34.0	30.0	35.0	21.0	39.0
35	31.667289410716467	34.0	30.0	35.0	21.0	39.0
36	31.551444646437066	34.0	30.0	35.0	21.0	38.0
37	31.45884949234384	34.0	30.0	35.0	21.0	37.0
38	31.178592215220345	34.0	30.0	35.0	21.0	37.0
39	31.070959588701506	34.0	30.0	35.0	21.0	37.0
40	30.85302222477707	33.0	30.0	35.0	20.0	36.0
41	30.663212982057786	33.0	29.0	35.0	20.0	36.0
42	30.61068107840108	33.0	29.0	35.0	20.0	36.0
43	30.318900491488765	33.0	29.0	35.0	18.0	36.0
44	30.153467636470765	33.0	29.0	35.0	18.0	35.0
45	30.063343848127097	33.0	29.0	35.0	20.0	35.0
46	29.964058536743096	33.0	28.0	35.0	18.0	35.0
47	29.95256217261028	33.0	29.0	35.0	18.0	35.0
48	29.82548151887274	33.0	28.0	35.0	18.0	35.0
49	29.808001422730637	33.0	28.0	35.0	18.0	35.0
50	29.647368487234946	33.0	27.0	35.0	18.0	35.0
51	29.44448476312972	33.0	27.0	35.0	17.0	35.0
52	28.918989232515862	33.0	26.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	5.0
13	15.0
14	36.0
15	181.0
16	519.0
17	1109.0
18	2314.0
19	4304.0
20	7740.0
21	12389.0
22	18466.0
23	26242.0
24	35840.0
25	47584.0
26	60241.0
27	75397.0
28	92357.0
29	110014.0
30	132375.0
31	155606.0
32	182118.0
33	209004.0
34	259591.0
35	330352.0
36	270969.0
37	136949.0
38	50158.0
39	4826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.639325208918653	21.223386673756366	33.92197615848357	16.215311958841408
2	23.132486401425606	21.67409767979938	34.02598639064735	21.16742952812767
3	21.586793754356215	19.899771141561697	40.59304694292551	17.92038816115658
4	31.977891987439733	17.85500003592754	30.910350006107684	19.256757970525047
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	0.0	100.0
11	99.06206662403265	0.6532525203170246	0.15574589168564837	0.12893496396467605
12	99.01549554857763	0.6994194109320322	0.15489261257895076	0.13019242791138833
13	96.5331269894876	2.0567619225545917	0.594466080808226	0.8156450071495807
14	59.782350954594776	25.747607225747117	4.889693466217333	9.58034835344078
15	34.69679849679167	27.185113063972583	18.54157535083244	19.576513088403306
16	32.38364865738778	18.245083315968355	24.099116901033995	25.27215112560987
17	23.934209486308013	18.06558033757518	29.305287096982806	28.694923079134004
18	22.761265080585474	20.82822862850204	30.139928791613073	26.270577499299414
19	25.201418778607305	20.887284524570845	27.368792619045905	26.54250407777594
20	27.524628329584893	19.637994093512205	26.79305376915836	26.04432380774454
21	32.16893668848666	19.626227823725113	21.149959761153706	27.054875726634524
22	30.85277612111893	20.056864316047395	21.955634875582923	27.134724687250756
23	31.24276958230641	20.313431870603367	20.703110965804168	27.74068758128606
24	29.524445099124087	22.23838462588651	20.268073349668388	27.96909692532101
25	32.339906875812865	18.617157915915183	22.747118611184963	26.295816597086997
26	30.979779979736865	20.14205749843715	21.315271360719702	27.56289116110628
27	31.360342461324002	22.845155889601852	19.0561026521711	26.738398996903044
28	31.346106173070154	22.711235979276996	19.247237172071365	26.695420675581488
29	32.28210844369077	20.972747163520612	19.50479273401404	27.240351658774582
30	31.197366151944756	20.993809684628044	19.29511062089977	28.51371354252743
31	30.92921196530837	21.18512384223498	19.835550661426034	28.050113531030618
32	31.426763503366413	23.028476169261832	19.41735408029087	26.127406247080888
33	30.95566361761599	23.214580833375248	18.699746351558176	27.130009197450583
34	29.794575300533882	24.990973205239673	19.127463731147024	26.08698776307942
35	30.642914370297987	24.620200978666226	19.19051656618931	25.54636808484648
36	32.04202264872206	23.457765378784067	19.74532762324943	24.754884349244442
37	32.284892828144194	24.46467963411392	18.786466454454658	24.463961083287224
38	30.183131660067975	26.061479208731832	20.861551423089914	22.893837708110283
39	31.31749886828245	25.836348252843667	20.744427638338998	22.10172524053489
40	31.831711803634434	25.830510027376786	18.14012998584455	24.197648183144235
41	31.46363414266108	26.63735278689938	17.82787474221989	24.071138328219646
42	33.08356207201316	25.86666211584476	17.55783435966343	23.491941452478642
43	31.882145089782927	24.596758257945375	19.03423176138364	24.486864890888057
44	31.63195467381385	25.42524736112209	19.46078149587911	23.482016469184945
45	28.70974318993454	25.928232939807	19.1286313762404	26.233392494018066
46	29.521256529830637	24.246330001652666	20.329239988790608	25.90317347972609
47	29.76776437281291	23.809316370743485	21.43854773692417	24.98437151951943
48	29.271021204434895	23.587239255868763	21.52338164390058	25.618357895795757
49	28.509132781007263	23.22971531016246	20.894649670544446	27.366502238285822
50	29.26612607692805	23.8391811394779	19.93161192506952	26.96308085852453
51	27.204693573999954	25.455291767563175	21.76081778269586	25.579196875741005
52	27.660614073536493	25.8401206446838	21.75232990105555	24.746935380724157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	6.5
12	11.0
13	59.0
14	191.0
15	275.0
16	528.5
17	782.0
18	1276.5
19	1771.0
20	1898.5
21	2026.0
22	2242.5
23	2459.0
24	2955.5
25	3452.0
26	4286.5
27	5121.0
28	7056.0
29	8991.0
30	11278.0
31	13565.0
32	15881.0
33	18197.0
34	19094.0
35	19991.0
36	20436.0
37	20881.0
38	20505.0
39	22669.5
40	25210.0
41	27212.5
42	29215.0
43	30964.5
44	32714.0
45	34269.5
46	35825.0
47	39324.5
48	42824.0
49	45278.0
50	47732.0
51	54073.5
52	60415.0
53	73427.0
54	86439.0
55	93475.5
56	100512.0
57	114933.0
58	129354.0
59	152495.0
60	175636.0
61	187577.0
62	199518.0
63	211304.0
64	216507.5
65	209925.0
66	194040.0
67	178155.0
68	168198.5
69	158242.0
70	140205.5
71	122169.0
72	103876.5
73	85584.0
74	77375.0
75	69166.0
76	56810.5
77	44455.0
78	37749.5
79	31044.0
80	21512.5
81	11981.0
82	9162.0
83	6343.0
84	4028.0
85	1713.0
86	1307.0
87	901.0
88	620.5
89	271.0
90	202.0
91	157.5
92	113.0
93	98.0
94	83.0
95	63.0
96	43.0
97	22.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2226704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.643529303404277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3808345372718	11.331285180684173
2	8.33445918149669	2.4409179450454723
3	3.738964819959038	1.6425492271640358
4	2.123618127852726	1.2438905713780768
5	1.3998797126949496	1.0249589797044827
6	0.9281914899172284	0.8155199569064046
7	0.6657631520641855	0.6824385558465089
8	0.4887943447528865	0.5726139448581749
9	0.36740740780968906	0.4842127028294088
>10	2.539063731131199	7.385801449384756
>50	0.4415464775970471	4.74069786825404
>100	1.4226233087278097	49.53097513073561
>500	0.15036607638269306	14.516185730081268
>1k	0.01848763234213439	3.587952757127594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.003952029546809993	0.0	0.0	0.0	0.0
12	0.01585302761390827	0.0	0.0	0.0	0.0
13	0.01809849894732304	0.0	0.0	0.0	0.0
14	0.0427537741882172	0.0	0.0	0.0	0.0
15	0.07163053553593113	0.0	0.0	0.0	0.0
16	0.09430979600342031	0.0	0.0	0.0	0.0
17	0.11249811380407994	0.0	0.0	0.0	0.0
18	0.13378518204485193	0.0	0.0	0.0	0.0
19	0.15893446097909736	0.0	0.0	0.0	0.0
20	0.18623939239342094	0.0	0.0	0.0	0.0
21	0.21331977667440308	0.0	0.0	0.0	0.0
22	0.2430498171288146	0.0	0.0	0.0	0.0
23	0.2678398206497137	0.0	0.0	0.0	0.0
24	0.2936627409839835	0.0	0.0	0.0	0.0
25	0.32177604207833643	0.0	0.0	0.0	0.0
26	0.3542904669861823	0.0	0.0	0.0	0.0
27	0.3930922116275895	0.0	0.0	0.0	0.0
28	0.43045685461561123	0.0	0.0	0.0	0.0
29	0.47707283949730184	0.0	0.0	0.0	0.0
30	0.5373412900861543	0.0	0.0	0.0	0.0
31	0.5883134893546695	0.0	0.0	0.0	0.0
32	0.6225344724759105	0.0	0.0	0.0	0.0
33	0.6515010526769611	0.0	0.0	0.0	0.0
34	0.6834316550381191	0.0	0.0	0.0	0.0
35	0.7144640688659112	0.0	0.0	0.0	0.0
36	0.7445982941603374	0.0	0.0	0.0	0.0
37	0.7800767412282907	0.0	0.0	0.0	0.0
38	0.819327580136381	0.0	0.0	0.0	0.0
39	0.8598807924178516	0.0	0.0	0.0	0.0
40	0.9026345666060689	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	105	0.0	46.000004	23
CATATAG	105	0.0	46.000004	42
ATCCGAT	105	0.0	46.000004	25
TCGGACA	105	0.0	46.000004	17
CCATATA	105	0.0	46.000004	41
AACGACC	55	1.8189894E-12	46.000004	35
TCCCGAT	105	0.0	46.000004	40
ATTGATC	55	1.8189894E-12	46.000004	14
ACCTTAT	55	1.8189894E-12	46.000004	45
TCACGAA	30	1.862194E-6	46.0	38
CGGAATA	75	0.0	46.0	13
ACTATTG	40	5.6152203E-9	46.0	18
AACCGTC	80	0.0	46.0	34
TAATAGC	30	1.862194E-6	46.0	41
TCGCATA	25	3.418195E-5	46.0	31
CATATGG	30	1.862194E-6	46.0	44
ATTAGCA	40	5.6152203E-9	46.0	26
CTCAAAC	25	3.418195E-5	46.0	15
TCACATA	40	5.6152203E-9	46.0	29
TAGGTTA	20	6.3127367E-4	46.0	22
>>END_MODULE