FastQCFastQC Report
Fri 17 Jun 2016
SRR1527944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233856
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA1400.046.00000423
CATATAG700.046.00000442
TCACATA351.0201438E-746.00000429
TATGGAC351.0201438E-746.00000414
GAATACC351.0201438E-746.00000425
GCTAGTT351.0201438E-746.00000428
TCATATC351.0201438E-746.00000417
CGCGTTA700.046.00000444
AATAGGT351.0201438E-746.00000439
CCTATAT351.0201438E-746.00000417
TGCGTAT1400.046.00000421
TCACGAA551.8189894E-1246.038
ATTTACG405.6152203E-946.030
ACTATTA800.046.024
GTACCTA206.31274E-446.044
CTACTTA405.6152203E-946.041
CATATCG301.8621977E-646.028
CATATAA206.31274E-446.032
CCGATAT1100.046.031
TAGAATC301.8621977E-646.046
Produced by FastQC (version 0.11.5)