##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2233856
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.200191507420353	31.0	31.0	33.0	30.0	34.0
2	31.722190239657348	31.0	31.0	34.0	30.0	34.0
3	31.974985406400414	31.0	31.0	34.0	30.0	34.0
4	35.80174147304034	37.0	35.0	37.0	35.0	37.0
5	35.82452181340248	37.0	35.0	37.0	35.0	37.0
6	35.788922831194135	37.0	35.0	37.0	35.0	37.0
7	34.93274006918978	35.0	35.0	36.0	33.0	37.0
8	35.343831921126515	35.0	35.0	37.0	35.0	37.0
9	37.212463113110246	39.0	37.0	39.0	35.0	39.0
10	37.247174392619755	39.0	37.0	39.0	35.0	39.0
11	38.01329539594316	39.0	37.0	39.0	35.0	39.0
12	37.97561704962182	39.0	37.0	39.0	35.0	39.0
13	37.661485789594316	39.0	37.0	39.0	35.0	39.0
14	37.439755740746044	40.0	37.0	40.0	31.0	40.0
15	34.67343776859386	37.0	32.0	39.0	25.0	40.0
16	33.7169786235102	36.0	31.0	39.0	23.0	40.0
17	33.435029384168004	35.0	31.0	39.0	23.0	40.0
18	33.43722872020399	35.0	31.0	39.0	23.0	40.0
19	33.62851455062457	35.0	31.0	39.0	24.0	40.0
20	33.586926373051796	35.0	31.0	39.0	24.0	40.0
21	33.36330721407288	35.0	31.0	39.0	24.0	40.0
22	33.166457909551916	35.0	31.0	38.0	23.0	40.0
23	32.776113142476504	35.0	30.0	38.0	23.0	40.0
24	32.54315005085377	34.0	30.0	38.0	22.0	40.0
25	32.346857183274125	34.0	30.0	37.0	22.0	40.0
26	32.705267483669495	35.0	30.0	37.0	23.0	40.0
27	32.86515827340706	35.0	31.0	37.0	23.0	40.0
28	32.77223957139583	35.0	31.0	37.0	23.0	40.0
29	32.738202462468486	35.0	31.0	37.0	23.0	40.0
30	32.70078420453243	35.0	31.0	37.0	23.0	40.0
31	32.536973735101995	34.0	31.0	36.0	23.0	39.0
32	32.27684327011231	34.0	31.0	36.0	23.0	39.0
33	32.044469294350215	34.0	31.0	36.0	22.0	39.0
34	31.873345461838184	34.0	30.0	35.0	22.0	39.0
35	31.711583915883566	34.0	30.0	35.0	22.0	39.0
36	31.26435499871075	34.0	30.0	35.0	21.0	38.0
37	31.322237870301397	34.0	30.0	35.0	21.0	37.0
38	31.162875762806554	34.0	30.0	35.0	21.0	37.0
39	31.094105439204675	34.0	30.0	35.0	21.0	37.0
40	30.887066131388952	34.0	30.0	35.0	20.0	36.0
41	30.71980960276759	33.0	29.0	35.0	20.0	36.0
42	30.5394121196711	33.0	29.0	35.0	20.0	36.0
43	30.25050182285698	33.0	29.0	35.0	18.0	36.0
44	30.09189222581939	33.0	29.0	35.0	18.0	35.0
45	29.796704890556956	33.0	27.0	35.0	18.0	35.0
46	29.817619846579188	33.0	27.0	35.0	18.0	35.0
47	29.86443620358698	33.0	28.0	35.0	18.0	35.0
48	29.728617690665825	33.0	27.0	35.0	18.0	35.0
49	29.724109790425167	33.0	27.0	35.0	18.0	35.0
50	29.514739087926884	33.0	27.0	35.0	17.0	35.0
51	29.387355765098555	33.0	27.0	35.0	17.0	35.0
52	29.001400716966536	33.0	27.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	9.0
13	4.0
14	36.0
15	171.0
16	457.0
17	1080.0
18	2180.0
19	4421.0
20	7482.0
21	11931.0
22	18234.0
23	26002.0
24	35625.0
25	46925.0
26	60394.0
27	75355.0
28	93326.0
29	110511.0
30	132347.0
31	156684.0
32	183788.0
33	211807.0
34	263386.0
35	330680.0
36	270695.0
37	135564.0
38	49918.0
39	4838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.703327340705936	21.185295739743296	33.842020255558104	16.269356663992664
2	23.250424378294753	21.767651988310796	33.8692377664451	21.112685866949345
3	21.752610732294293	19.900432257047903	40.465544779967914	17.881412230689893
4	31.884060566124226	17.812159781113913	31.081457354457942	19.222322298303922
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	0.0	100.0
11	99.07232158205363	0.6479826810680724	0.15108404480861792	0.12861169206967682
12	98.99742866147146	0.7051484070593629	0.16478233153793262	0.13264059993123997
13	96.53222947226678	2.052683789823516	0.6025903191611276	0.8124964187485675
14	59.74977796241119	25.692703558331427	4.98161027389411	9.575908205363282
15	34.52872521774009	27.240162302314918	18.669959030483614	19.56115344946138
16	32.230860001719	18.190071338528536	24.318801211895487	25.260267447856975
17	23.820380543777215	18.057833629383452	29.499081409007566	28.62270441783177
18	22.683422745244098	20.778778936511575	30.21864435308274	26.319153965161586
19	25.178256790052718	20.805772619184047	27.47092919149668	26.545041399266562
20	27.501548891244553	19.6156780025212	26.849134411528762	26.03363869470548
21	31.888044708342882	19.6149617522347	21.1774617522347	27.319531787187717
22	30.821503266101306	20.051426770570707	21.956652532661014	27.170417430666973
23	31.196191697226677	20.314603985216596	20.721255085377035	27.767949232179696
24	29.38143729944992	22.26325242092597	20.40234464531286	27.952965634311255
25	32.56843771487509	18.48221192413477	22.577731062342423	26.37161929864772
26	30.869805394797154	20.229817857552142	21.326665640041256	27.573711107609444
27	31.32149073172129	22.855098985789592	19.091293261517308	26.732117020971806
28	31.41549858182443	22.73817112651845	19.274429506646804	26.571900785010317
29	32.18707920295668	21.026601535640616	19.535368439147376	27.250950822255326
30	31.13786206451983	21.037703415081367	19.360290009741004	28.464144510657803
31	30.84442327526931	21.2883462497135	19.81161722152189	28.055613253495302
32	31.391504197226677	23.15596887176255	19.341040783291312	26.11148614771946
33	30.89836587497135	23.280730718542287	18.77887383967454	27.042029566811827
34	29.757468699862482	25.03979665654366	19.20387885342654	25.998855790167312
35	30.60734443043777	24.69138565780426	19.161351492665595	25.539918419092366
36	32.1099480002292	23.400568344602338	19.770029939261978	24.719453715906486
37	32.216803589846435	24.497953314806324	18.843023005959203	24.442220089388037
38	30.15346557701123	26.115291227366487	20.926416026816412	22.804827168805865
39	31.309941195851476	25.827269080907634	20.720538835090537	22.142250888150354
40	31.766595519138207	25.86970691038276	18.15296062055925	24.21073694991978
41	31.415946238253493	26.7062872450149	17.814980016617007	24.0627865001146
42	33.25984306956223	25.840027289135914	17.577140155856064	23.322989485445795
43	31.854425710520285	24.643755013752006	19.055435981549394	24.446383294178318
44	31.879807830048136	25.373524524409806	19.366512434104973	23.380155211437085
45	28.766984084918633	25.92678310508824	19.192732208342882	26.11350060165024
46	29.507900240660096	24.294941124226447	20.253230288792114	25.94392834632134
47	29.737816582626632	23.882560021774008	21.4550535038964	24.92456989170296
48	29.332821811253723	23.682054707196883	21.44180287359615	25.54332060795324
49	28.54955735732294	23.305262290854916	20.942173533119412	27.20300681870273
50	29.27856585205134	23.854939620100847	19.95370337210635	26.912791155741463
51	27.17238711895485	25.515744971922988	21.744866276071512	25.56700163305065
52	27.551910239514093	25.839803460921384	21.82356427630071	24.784722023263807
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	6.0
12	12.0
13	63.5
14	176.5
15	238.0
16	479.0
17	720.0
18	1240.0
19	1760.0
20	1836.0
21	1912.0
22	2175.5
23	2439.0
24	2882.0
25	3325.0
26	4231.0
27	5137.0
28	7033.5
29	8930.0
30	11318.5
31	13707.0
32	16186.5
33	18666.0
34	19428.5
35	20191.0
36	20636.5
37	21082.0
38	20990.0
39	23237.5
40	25577.0
41	27480.0
42	29383.0
43	31095.5
44	32808.0
45	34533.0
46	36258.0
47	39819.0
48	43380.0
49	46066.0
50	48752.0
51	54943.5
52	61135.0
53	73825.5
54	86516.0
55	93991.5
56	101467.0
57	116006.0
58	130545.0
59	152878.0
60	175211.0
61	187856.5
62	200502.0
63	211023.0
64	215936.5
65	210329.0
66	194537.5
67	178746.0
68	168181.0
69	157616.0
70	139961.5
71	122307.0
72	104334.5
73	86362.0
74	77812.0
75	69262.0
76	56715.5
77	44169.0
78	37591.5
79	31014.0
80	21598.5
81	12183.0
82	9270.0
83	6357.0
84	4063.0
85	1769.0
86	1304.0
87	839.0
88	579.5
89	257.5
90	195.0
91	145.0
92	95.0
93	78.0
94	61.0
95	41.5
96	22.0
97	11.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2233856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.00225582791897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08675711954331	11.56475251252043
2	8.739597168327679	2.622273451044161
3	3.762529777339562	1.6933930283943335
4	2.150770347896377	1.2906562794497494
5	1.358798873864281	1.0192524162200072
6	0.9039430137042335	0.8136710607270584
7	0.6449021724747183	0.6772491162812513
8	0.48354129831701664	0.5803368208572776
9	0.37472284706594844	0.5059519214624563
>10	2.466052219977743	7.3168407094188685
>50	0.4370212784999995	4.818721061455969
>100	1.4343336204988322	50.1003264918079
>500	0.13968569700815467	13.630278591192438
>1k	0.017344565482165546	3.3662965391681308
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0042975017190006876	0.0	0.0	0.0	0.0
12	0.01607086580334632	0.0	0.0	0.0	0.0
13	0.01844344487737795	0.0	0.0	0.0	0.0
14	0.044899939834975935	0.0	0.0	0.0	0.0
15	0.07198315379326152	0.0	0.0	0.0	0.0
16	0.09494792860417144	0.0	0.0	0.0	0.0
17	0.11200363855145543	0.0	0.0	0.0	0.0
18	0.13393880357552143	0.0	0.0	0.0	0.0
19	0.15847037588815036	0.0	0.0	0.0	0.0
20	0.1883290597066239	0.0	0.0	0.0	0.0
21	0.21330828844831537	0.0	0.0	0.0	0.0
22	0.24231642505157003	0.0	0.0	0.0	0.0
23	0.2686833887233555	0.0	0.0	0.0	0.0
24	0.29590089961035987	0.0	0.0	0.0	0.0
25	0.3264310680724272	0.0	0.0	0.0	0.0
26	0.36058725360990146	0.0	0.0	0.0	0.0
27	0.40141351994040797	0.0	0.0	0.0	0.0
28	0.44018056669722666	0.0	0.0	0.0	0.0
29	0.4876769138207655	0.0	0.0	0.0	0.0
30	0.5481105317442126	0.0	0.0	0.0	0.0
31	0.6015607093742837	0.0	0.0	0.0	0.0
32	0.6364331451982581	0.0	0.0	0.0	0.0
33	0.667231907517763	0.0	0.0	0.0	0.0
34	0.6986126231950492	0.0	0.0	0.0	0.0
35	0.7301724014439606	0.0	0.0	0.0	0.0
36	0.7606130386202155	0.0	0.0	0.0	0.0
37	0.7954407088012835	0.0	0.0	0.0	0.0
38	0.8330438488425396	0.0	0.0	0.0	0.0
39	0.8767798819619528	0.0	0.0	0.0	0.0
40	0.9173823200779281	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	140	0.0	46.000004	23
CATATAG	70	0.0	46.000004	42
TCACATA	35	1.0201438E-7	46.000004	29
TATGGAC	35	1.0201438E-7	46.000004	14
GAATACC	35	1.0201438E-7	46.000004	25
GCTAGTT	35	1.0201438E-7	46.000004	28
TCATATC	35	1.0201438E-7	46.000004	17
CGCGTTA	70	0.0	46.000004	44
AATAGGT	35	1.0201438E-7	46.000004	39
CCTATAT	35	1.0201438E-7	46.000004	17
TGCGTAT	140	0.0	46.000004	21
TCACGAA	55	1.8189894E-12	46.0	38
ATTTACG	40	5.6152203E-9	46.0	30
ACTATTA	80	0.0	46.0	24
GTACCTA	20	6.31274E-4	46.0	44
CTACTTA	40	5.6152203E-9	46.0	41
CATATCG	30	1.8621977E-6	46.0	28
CATATAA	20	6.31274E-4	46.0	32
CCGATAT	110	0.0	46.0	31
TAGAATC	30	1.8621977E-6	46.0	46
>>END_MODULE