FastQCFastQC Report
Fri 17 Jun 2016
SRR1527943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385581
Sequences flagged as poor quality0
Sequence length59
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGCGTGGCGGATTTGCCACTGTTGGCTGGGCGCAGTGGCTCACACCTGTAATCCCAG16970.12247569792022263No Hit
TGCGCGTGGCGGGAGCTTCTGTCAACTGTGTTGCGCCAATGGTGGAGAAGAAACCCGCC16010.1155471964468335No Hit
GTTACGTGGCGGGGCATTACCATTGTTGCTATCACCCTTAGTCTTCTGGAAAGGTTGAA15910.11482547754335547No Hit
GTCGCGTGGCGGGCATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCA14950.10789697606996632No Hit
GCTGCGTGGCGGGACGCAGAGTGGTACGTGGCGGGGTCTCGCAACCCAAGATGGCGGAT14120.10190670917109862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAAGT750.053.00000444
TGCGTAT750.053.00000421
AACCGTT203.13847E-453.029
GATAATT401.6007107E-953.019
CATATAC401.6007107E-953.047
CATATAA503.6379788E-1253.032
ATTAGCG353.342757E-853.047
ATAGGTG401.6007107E-953.049
ACTGATA307.011531E-753.017
AGATAAT457.6397555E-1153.014
CCGATAA401.6007107E-953.048
CTATCAG401.6007107E-953.033
TCCGAAT550.053.052
TTATGTA307.011531E-753.023
ATAATAT150.00669655653.045
GACGTAA203.13847E-453.013
TATGGAC401.6007107E-953.014
ATTACAT307.011531E-753.048
CCAATTA150.00669655653.034
ATGCTTA150.00669655653.014