##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527943_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1385581
Sequences flagged as poor quality	0
Sequence length	59
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.782561972197943	31.0	31.0	31.0	30.0	33.0
2	31.285549527598892	31.0	31.0	31.0	30.0	34.0
3	31.466156796318657	31.0	31.0	34.0	30.0	34.0
4	35.50140987787795	35.0	35.0	37.0	35.0	37.0
5	35.45584126803124	35.0	35.0	37.0	35.0	37.0
6	35.42742719480131	35.0	35.0	37.0	35.0	37.0
7	34.53836838120615	35.0	35.0	35.0	32.0	37.0
8	35.017908011152	35.0	35.0	37.0	35.0	37.0
9	36.803621729801435	37.0	35.0	39.0	35.0	39.0
10	36.67047253101767	37.0	35.0	39.0	35.0	39.0
11	37.414885163696674	39.0	37.0	39.0	35.0	39.0
12	37.43273399389859	39.0	37.0	39.0	35.0	39.0
13	36.36475168178548	37.0	35.0	39.0	32.0	39.0
14	35.16728578119937	38.0	33.0	40.0	25.0	40.0
15	31.156182135869358	35.0	26.0	38.0	15.0	39.0
16	29.822617371340975	32.0	25.0	37.0	15.0	39.0
17	29.44436305059033	32.0	24.0	37.0	15.0	39.0
18	29.340556777265277	32.0	24.0	37.0	15.0	39.0
19	30.753544541964708	33.0	26.0	37.0	18.0	40.0
20	31.09964123353308	33.0	27.0	38.0	20.0	40.0
21	31.094924800498852	33.0	27.0	37.0	20.0	40.0
22	31.148003617255146	33.0	28.0	37.0	20.0	40.0
23	30.837728721742	33.0	27.0	37.0	19.0	39.0
24	30.706375881308997	33.0	27.0	37.0	19.0	39.0
25	30.604313280854747	33.0	27.0	37.0	18.0	39.0
26	29.99416706782209	33.0	26.0	36.0	17.0	39.0
27	29.678811992947363	33.0	26.0	36.0	15.0	39.0
28	29.53356606362241	32.0	26.0	36.0	15.0	39.0
29	29.225574686719867	32.0	25.0	35.0	15.0	39.0
30	29.148912261354624	32.0	25.0	35.0	15.0	39.0
31	29.027612243528168	32.0	24.0	35.0	15.0	39.0
32	28.668445222617805	32.0	23.0	35.0	13.0	38.0
33	28.33145373673571	31.0	23.0	35.0	12.0	38.0
34	28.17138658800893	31.0	23.0	35.0	12.0	37.0
35	28.037688161139624	31.0	23.0	35.0	12.0	37.0
36	27.788666992402465	31.0	23.0	34.0	11.0	37.0
37	27.71885584458794	31.0	23.0	34.0	11.0	36.0
38	27.370573066460928	31.0	22.0	34.0	10.0	36.0
39	27.26058166213307	31.0	22.0	34.0	10.0	36.0
40	27.060422306599182	31.0	22.0	34.0	10.0	36.0
41	26.841460730191883	31.0	21.0	34.0	9.0	35.0
42	26.73492347253607	30.0	21.0	34.0	9.0	35.0
43	26.396726716085166	30.0	20.0	34.0	9.0	35.0
44	26.11770224909262	30.0	20.0	34.0	9.0	35.0
45	26.025537301680668	30.0	20.0	34.0	8.0	35.0
46	25.90996845366673	30.0	20.0	34.0	8.0	35.0
47	25.85362818918562	30.0	20.0	34.0	8.0	35.0
48	25.733359507672233	30.0	20.0	34.0	8.0	35.0
49	25.755646187411635	30.0	20.0	34.0	8.0	35.0
50	25.47391238765543	29.0	18.0	33.0	8.0	35.0
51	25.291770744546874	29.0	18.0	33.0	8.0	35.0
52	25.146385523473548	29.0	18.0	33.0	8.0	35.0
53	25.054739491953196	29.0	18.0	33.0	8.0	35.0
54	24.854756235831754	29.0	18.0	33.0	8.0	35.0
55	24.85424237197248	29.0	18.0	33.0	8.0	35.0
56	24.77711010760107	29.0	18.0	33.0	8.0	35.0
57	24.674137419609536	29.0	18.0	33.0	8.0	35.0
58	24.411592682059005	29.0	17.0	33.0	8.0	35.0
59	24.063589930866545	27.0	15.0	33.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	16.0
13	124.0
14	647.0
15	1986.0
16	4844.0
17	9715.0
18	16045.0
19	23444.0
20	30996.0
21	38685.0
22	46574.0
23	53944.0
24	61568.0
25	69186.0
26	76523.0
27	82993.0
28	87089.0
29	90611.0
30	94114.0
31	96553.0
32	98803.0
33	99966.0
34	102758.0
35	93578.0
36	62038.0
37	29879.0
38	12199.0
39	699.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.473398523796156	21.517038700732762	34.058059398909194	15.95150337656189
2	22.699069920849087	22.542673434465396	33.030331680356475	21.72792496432904
3	15.506419328786986	18.077182062975748	46.835443037974684	19.580955570262585
4	37.5738408653121	22.622784232751457	18.826831488018385	20.976543413918062
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	0.0	100.0
11	98.59481329492826	0.9589479070512658	0.23455864363036155	0.21168015439010784
12	98.4494591077678	1.1035082034179164	0.24127062943270727	0.20576205938158793
13	94.49227435999772	3.2782637752682806	0.9454517635562265	1.2840101011777731
14	58.93008059434995	26.97460487694332	3.906159221294172	10.189155307412557
15	36.556866758421194	26.104933598252284	17.708528047079167	19.62967159624735
16	34.435229697866816	19.05078086376762	21.559547944147617	24.95444149421795
17	24.67968310766386	18.251332834384996	28.658663766318966	28.410320291632175
18	23.274135543140385	23.613704287226803	27.84571959344131	25.266440576191503
19	26.647738385558117	22.881520459648335	24.573301741291196	25.897439413502347
20	28.88990250299333	20.7912059995049	25.033397542258445	25.285493955243325
21	31.781180602216686	21.349383399454812	20.74746983395413	26.121966164374367
22	31.256707475059198	20.918228526517034	21.769640316950074	26.055423681473695
23	30.783404218158307	21.528225343736672	20.673782333909024	27.014588104196
24	29.15953668533272	23.83072516150265	20.0798076763466	26.929930476818026
25	31.58234704430849	19.989809329082888	22.685429433573354	25.742414193035266
26	30.568620672483238	21.58372552741413	20.81985823997298	27.027795560129647
27	31.215425153780256	24.536638420994514	18.915891600707575	25.33204482451766
28	31.197021321741563	23.909609037652796	18.71222252614607	26.18114711445957
29	32.57673134952053	21.550382114073447	19.516217384620603	26.35666915178542
30	31.028066926437358	22.038047577153556	19.545519172101812	27.388366324307277
31	31.04228478883587	21.698623176847835	19.8219375121339	27.43715452218239
32	31.474738755799912	23.597465611898546	19.11811723746212	25.80967839483942
33	31.17269939469435	23.679308535552956	18.278325121375076	26.869666948377613
34	30.051436906250878	25.18871145028692	18.670146314073303	26.089705329388902
35	30.91879868445078	24.249610813081297	19.532600403729557	25.29899009873836
36	32.29143586697566	23.593496157929415	20.046608606786613	24.06845936830831
37	32.9536851328071	24.854916457428327	18.56311540068751	23.62828300907706
38	30.18517141906536	25.924431700492427	20.978780742518843	22.911616137923367
39	31.48202811672504	25.972786867025455	20.160640193536143	22.38454482271336
40	32.41420025245727	26.197602305458865	17.708455875188818	23.679741566895043
41	31.971209189502453	26.548285520658844	17.2243990066261	24.256106283212603
42	33.32262783626508	25.754827758175093	17.484795187000977	23.437749218558857
43	32.127028300763364	24.723274929433934	18.470951896713363	24.67874487308934
44	32.270866878226535	25.410856528777458	19.068318633122132	23.249957959873875
45	29.408096675690558	25.936123546728773	18.289511764378986	26.366268013201683
46	30.07135634798687	24.027970937823195	20.404292495350326	25.496380218839604
47	30.73988456827858	23.918125320713838	20.775328183628382	24.5666619273792
48	30.846410278431936	23.20001501175319	20.7469646307217	25.206610079093174
49	29.45659618600428	22.829917558049655	20.76103814933952	26.952448106606543
50	30.202131813297093	23.307118097029335	19.777696143350695	26.71305394632288
51	28.253562945796745	24.985547578957853	21.172129236760608	25.58876023848479
52	28.112972103399226	25.617628994623914	21.66600148241063	24.603397419566235
53	29.196055661848714	25.282679251519756	20.659275783949116	24.861989302682414
54	28.206218185728588	24.974288764063594	20.33782218434	26.48167086586782
55	28.269729449234653	26.054701962570213	20.136246094598583	25.53932249359655
56	29.498239366734964	24.451836449835845	20.35976243900573	25.690161744423456
57	29.394095328963083	24.216916946753745	19.917204407393	26.471783316890168
58	26.864037540930486	23.939993403489222	22.053853221139725	27.142115834440574
59	29.50762171248018	24.61075895238171	20.261319980571326	25.620299354566782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	5.0
10	10.0
11	39.0
12	68.0
13	122.5
14	344.5
15	512.0
16	809.0
17	1106.0
18	1267.0
19	1408.5
20	1389.0
21	1281.0
22	1173.0
23	1404.0
24	1635.0
25	1979.0
26	3225.0
27	4127.0
28	5009.5
29	5892.0
30	6962.5
31	8998.0
32	9963.0
33	10929.5
34	11896.0
35	12499.5
36	14339.5
37	15576.0
38	16040.5
39	16505.0
40	16698.0
41	16891.0
42	15890.5
43	16635.5
44	18381.0
45	20758.5
46	23136.0
47	25956.5
48	30631.0
49	32485.0
50	34662.0
51	36839.0
52	36953.0
53	43051.0
54	49035.0
55	55358.5
56	61682.0
57	63184.5
58	77352.0
59	90017.0
60	95653.0
61	101289.0
62	111063.5
63	120838.0
64	115615.0
65	105751.5
66	101111.0
67	93671.0
68	86231.0
69	80720.5
70	67135.5
71	59061.0
72	50040.5
73	41020.0
74	40012.0
75	35191.0
76	31378.0
77	26895.0
78	22412.0
79	17402.5
80	12393.0
81	9398.0
82	5387.5
83	4372.0
84	3219.0
85	2066.0
86	1678.0
87	1064.5
88	839.0
89	751.5
90	664.0
91	528.0
92	302.0
93	212.0
94	167.5
95	123.0
96	100.5
97	39.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1385581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.96159835994835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.94183131099335	28.65741724351344
2	5.635915106651076	3.7153754027239647
3	2.215324724064204	2.1906193137440306
4	1.1438262803729888	1.508093697888325
5	0.736239778489697	1.2133819937597365
6	0.4790757643050641	0.9474661756205282
7	0.3199671897271096	0.7382640997302463
8	0.23273567753123164	0.6137071941451929
9	0.17739022601625568	0.5262358844635452
>10	1.3150156287002694	8.865986959171728
>50	0.24927280956149664	5.939167979384238
>100	0.5127965127023758	35.881698082745096
>500	0.03715290655424258	7.744007566237087
>1k	0.0034560843306272173	1.4585784068728551
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCGCGTGGCGGATTTGCCACTGTTGGCTGGGCGCAGTGGCTCACACCTGTAATCCCAG	1697	0.12247569792022263	No Hit
TGCGCGTGGCGGGAGCTTCTGTCAACTGTGTTGCGCCAATGGTGGAGAAGAAACCCGCC	1601	0.1155471964468335	No Hit
GTTACGTGGCGGGGCATTACCATTGTTGCTATCACCCTTAGTCTTCTGGAAAGGTTGAA	1591	0.11482547754335547	No Hit
GTCGCGTGGCGGGCATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCA	1495	0.10789697606996632	No Hit
GCTGCGTGGCGGGACGCAGAGTGGTACGTGGCGGGGTCTCGCAACCCAAGATGGCGGAT	1412	0.10190670917109862	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.006206782569911106	0.0	0.0	0.0	0.0
12	0.021579395213993262	0.0	0.0	0.0	0.0
13	0.024610614608601013	0.0	7.217189034780355E-5	0.0	0.0
14	0.0466230411646811	0.0	7.217189034780355E-5	0.0	0.0
15	0.07166668711536893	0.0	7.217189034780355E-5	0.0	0.0
16	0.09086440994788468	0.0	7.217189034780355E-5	0.0	0.0
17	0.10602050692092342	0.0	7.217189034780355E-5	0.0	0.0
18	0.1295485431743074	0.0	7.217189034780355E-5	0.0	0.0
19	0.15184965729177868	0.0	7.217189034780355E-5	0.0	0.0
20	0.17790370970733577	0.0	7.217189034780355E-5	0.0	0.0
21	0.20330821510976263	0.0	7.217189034780355E-5	0.0	0.0
22	0.22763014215697241	0.0	7.217189034780355E-5	0.0	0.0
23	0.2505086313972261	0.0	7.217189034780355E-5	0.0	0.0
24	0.2743253552120013	0.0	7.217189034780355E-5	0.0	0.0
25	0.30131764220207985	0.0	7.217189034780355E-5	0.0	0.0
26	0.3283099291921584	0.0	7.217189034780355E-5	0.0	0.0
27	0.36389067113362555	0.0	7.217189034780355E-5	0.0	0.0
28	0.39413069318935523	0.0	7.217189034780355E-5	0.0	0.0
29	0.42718541896864926	0.0	7.217189034780355E-5	0.0	0.0
30	0.47330325690089575	0.0	7.217189034780355E-5	0.0	0.0
31	0.5065744983512331	0.0	7.217189034780355E-5	0.0	0.0
32	0.5321233475343556	0.0	7.217189034780355E-5	0.0	0.0
33	0.5544966335421747	0.0	7.217189034780355E-5	0.0	0.0
34	0.5756429974140812	0.0	7.217189034780355E-5	0.0	0.0
35	0.592964251097554	0.0	7.217189034780355E-5	0.0	0.0
36	0.6119454582590264	0.0	7.217189034780355E-5	0.0	0.0
37	0.6314318686529333	0.0	7.217189034780355E-5	0.0	0.0
38	0.6546712173449261	0.0	7.217189034780355E-5	0.0	0.0
39	0.6792096600631793	0.0	7.217189034780355E-5	0.0	0.0
40	0.7023046649744764	0.0	7.217189034780355E-5	0.0	0.0
41	0.7257605293375126	0.0	7.217189034780355E-5	0.0	0.0
42	0.7440921894858546	0.0	7.217189034780355E-5	0.0	0.0
43	0.7630012247569792	0.0	7.217189034780355E-5	0.0	0.0
44	0.7809720254535822	0.0	7.217189034780355E-5	0.0	7.217189034780355E-5
45	0.7959116067555776	0.0	7.217189034780355E-5	0.0	7.217189034780355E-5
46	0.8127998290969637	0.0	7.217189034780355E-5	0.0	7.217189034780355E-5
47	0.8295437076576541	0.0	7.217189034780355E-5	0.0	7.217189034780355E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAAGT	75	0.0	53.000004	44
TGCGTAT	75	0.0	53.000004	21
AACCGTT	20	3.13847E-4	53.0	29
GATAATT	40	1.6007107E-9	53.0	19
CATATAC	40	1.6007107E-9	53.0	47
CATATAA	50	3.6379788E-12	53.0	32
ATTAGCG	35	3.342757E-8	53.0	47
ATAGGTG	40	1.6007107E-9	53.0	49
ACTGATA	30	7.011531E-7	53.0	17
AGATAAT	45	7.6397555E-11	53.0	14
CCGATAA	40	1.6007107E-9	53.0	48
CTATCAG	40	1.6007107E-9	53.0	33
TCCGAAT	55	0.0	53.0	52
TTATGTA	30	7.011531E-7	53.0	23
ATAATAT	15	0.006696556	53.0	45
GACGTAA	20	3.13847E-4	53.0	13
TATGGAC	40	1.6007107E-9	53.0	14
ATTACAT	30	7.011531E-7	53.0	48
CCAATTA	15	0.006696556	53.0	34
ATGCTTA	15	0.006696556	53.0	14
>>END_MODULE