FastQCFastQC Report
Fri 17 Jun 2016
SRR1527942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4248481
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGAGATCGGGGAGTGCTGGAGATCGGGGGGCGTGGTGGCACAAGCCTAA63900.15040669829993356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA351.0204349E-746.00000416
ACTAGGT351.0204349E-746.00000428
ACGATAA351.0204349E-746.00000418
TAACACG351.0204349E-746.00000433
TAGATAA351.0204349E-746.00000431
GCGTAAC700.046.00000431
AGGTATA253.4187236E-546.015
AACCGAT206.313387E-446.028
ATTAGTA453.110472E-1046.033
CGTATAG253.4187236E-546.046
CGTATAC301.8625979E-646.029
CCGATAT206.313387E-446.045
ATAATAC301.8625979E-646.016
ACTCTAT405.6170393E-946.046
ACCGATA206.313387E-446.044
TACGGTA501.6370905E-1146.033
CGACAAT551.8189894E-1246.016
CCTAACG600.046.032
AATATCG206.313387E-446.030
CGTTAAT405.6170393E-946.018
Produced by FastQC (version 0.11.5)