##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527942_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4248481
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64461156822874	31.0	31.0	34.0	30.0	34.0
2	32.26550289385783	34.0	31.0	34.0	30.0	34.0
3	32.50206932783741	34.0	31.0	34.0	31.0	34.0
4	36.013018770708875	37.0	35.0	37.0	35.0	37.0
5	35.96722240254811	37.0	35.0	37.0	35.0	37.0
6	35.81273236246084	37.0	35.0	37.0	35.0	37.0
7	35.93700171896732	37.0	35.0	37.0	35.0	37.0
8	35.679996685874315	37.0	35.0	37.0	33.0	37.0
9	37.74321622245692	39.0	38.0	39.0	35.0	39.0
10	37.663985551541835	39.0	37.0	39.0	35.0	39.0
11	38.18351523756373	39.0	39.0	39.0	37.0	39.0
12	38.16239380616272	39.0	39.0	39.0	37.0	39.0
13	38.10656655873005	39.0	39.0	39.0	37.0	39.0
14	38.71009685579387	40.0	39.0	40.0	36.0	40.0
15	38.13131681652807	40.0	38.0	40.0	34.0	40.0
16	37.6215120180601	40.0	37.0	40.0	33.0	40.0
17	37.62703869924333	40.0	37.0	40.0	33.0	40.0
18	37.669151868632575	40.0	37.0	40.0	33.0	40.0
19	37.775379482690404	40.0	37.0	40.0	33.0	40.0
20	37.76288207479332	40.0	37.0	40.0	33.0	40.0
21	37.61168897777817	40.0	37.0	40.0	33.0	40.0
22	37.62763867838882	40.0	37.0	40.0	33.0	40.0
23	37.53077040947105	40.0	37.0	40.0	33.0	40.0
24	37.515736565610155	40.0	37.0	40.0	33.0	40.0
25	37.63209580082858	40.0	37.0	40.0	33.0	40.0
26	37.622720685346124	40.0	37.0	40.0	33.0	40.0
27	37.57033372633654	40.0	37.0	40.0	33.0	40.0
28	37.496259957382414	40.0	37.0	40.0	33.0	40.0
29	37.47436366080018	40.0	37.0	40.0	33.0	40.0
30	37.40347832554741	40.0	36.0	40.0	33.0	40.0
31	37.33801563429376	40.0	36.0	40.0	33.0	40.0
32	37.2140703936301	40.0	36.0	40.0	33.0	40.0
33	37.096868504296005	39.0	35.0	40.0	32.0	40.0
34	36.95264566323822	39.0	35.0	40.0	32.0	40.0
35	36.84102788737904	39.0	35.0	40.0	32.0	40.0
36	36.657265973414965	39.0	35.0	40.0	32.0	40.0
37	36.4759169218363	38.0	35.0	40.0	31.0	40.0
38	36.259317859724455	38.0	35.0	40.0	31.0	40.0
39	36.08849939543098	38.0	35.0	40.0	31.0	40.0
40	35.79463036318157	37.0	35.0	40.0	30.0	40.0
41	35.60476414982202	37.0	35.0	40.0	30.0	40.0
42	35.37229000200307	37.0	35.0	40.0	30.0	40.0
43	35.14724580385319	36.0	34.0	40.0	29.0	40.0
44	34.92489503895628	36.0	34.0	40.0	29.0	40.0
45	34.727837314089435	35.0	34.0	39.0	28.0	40.0
46	34.49447014121047	35.0	34.0	39.0	28.0	40.0
47	34.29098235345762	35.0	34.0	39.0	27.0	40.0
48	34.08742371685315	35.0	33.0	39.0	27.0	40.0
49	33.92318148533558	35.0	33.0	38.0	27.0	40.0
50	33.71593706079891	35.0	33.0	37.0	26.0	40.0
51	33.518909464347374	35.0	33.0	37.0	26.0	40.0
52	32.854543070805775	35.0	33.0	36.0	24.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	8.0
14	16.0
15	25.0
16	67.0
17	191.0
18	407.0
19	965.0
20	1888.0
21	3668.0
22	6236.0
23	9874.0
24	14798.0
25	21169.0
26	29355.0
27	39369.0
28	52342.0
29	67098.0
30	84669.0
31	104548.0
32	128420.0
33	163335.0
34	230190.0
35	381426.0
36	516547.0
37	921737.0
38	1117927.0
39	352199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.371368260797208	19.088610729340676	35.689908934510946	15.850112075351166
2	19.325518932531416	22.912000783338797	36.64203747174579	21.120442812384002
3	22.2415964670667	22.74707124734699	35.99679508982152	19.01453719576479
4	24.087715115119966	22.483118083851615	35.34915184980232	18.080014951226097
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	0.0	100.0
11	99.34884962413625	0.45519798723355476	0.16255221572133663	0.03340017290885849
12	99.07752912158487	0.5918821338732596	0.17441527924921874	0.156173465292654
13	96.09446293863618	2.3770848922238326	0.5637073579945396	0.9647448111454423
14	58.12074951023671	25.17061980505503	6.201204618780219	10.507426065928033
15	33.677307254051506	25.99110129008462	17.964608997898306	22.366982457965566
16	27.193813506521508	18.034186807002314	28.870412742813258	25.901586943662924
17	23.42008826213416	17.52506366393071	30.17483189874216	28.880016175192967
18	24.134884915337977	18.893646929337805	29.174898040028896	27.796570115295328
19	25.151012797279783	16.97903321210569	28.024769323435834	29.845184667178692
20	28.616321927766652	17.107290817588687	25.01489355842712	29.261493696217546
21	33.10536165749593	18.6114284140614	19.72163698037016	28.5615729480725
22	32.10639284958553	18.727163896931636	20.973637401226462	28.19280585225637
23	30.26695894367893	19.683176175202384	20.81838191108775	29.231482970030935
24	31.351064062661454	20.165254357969353	20.609954475493712	27.873727103875478
25	32.90696133512189	18.88183094145884	20.67197664294603	27.539231080473233
26	32.876220936377024	18.982054998009875	18.600624552634223	29.541099512978874
27	31.926469719412655	20.886406223777392	17.920969871349314	29.266154185460643
28	31.572484377357462	21.132588329805404	19.389023982924723	27.90590330991241
29	30.30603173228267	19.369063907782568	20.298243066168826	30.026661293765937
30	32.03241346730749	18.60003610702272	19.57770318379675	29.789847241873034
31	32.36914558403345	18.345450997662457	20.007739236682475	29.27766418162162
32	33.36368928094535	20.104056014373135	19.479621069271584	27.05263363540993
33	31.754926054747568	20.024921848538334	19.25389333269938	28.966258764014714
34	30.673833777295933	21.766579631637757	19.989120817534552	27.570465773531765
35	34.45753905925436	21.27814623626656	17.847649548156152	26.41666515632293
36	33.298089364175105	21.002753690083587	19.674019961487414	26.025136984253898
37	33.35987615338282	21.617090908491765	19.866300449501832	25.15673248862358
38	31.87654599373282	23.460997001045786	21.053477701795064	23.60897930342633
39	33.77875527747447	22.331204964786238	20.67701373738049	23.213026020358807
40	33.02269681799213	22.435571678442248	19.10930047704109	25.432431026524537
41	34.34860600765309	23.95875137490317	17.056237276334766	24.636405341108976
42	34.018535095249334	23.628139092536838	17.70352744898706	24.649798363226765
43	33.90192871287409	22.92878325217884	17.852027583505727	25.31726045144135
44	31.329903558471837	23.359572515447287	19.95550880420555	25.355015121875326
45	30.024613503037912	21.82114030873623	20.046458957919313	28.107787230306545
46	30.541645355128104	20.101231475437928	21.569403276135635	27.787719893298334
47	31.185404854111386	20.37829520715757	22.35109442645501	26.085205512276033
48	30.28609519496498	20.269009088189403	22.161332485657816	27.283563231187806
49	30.970480978966364	20.020449661890922	20.99569234274556	28.013377016397158
50	30.28722501053906	20.828503175605587	21.373733341398964	27.510538472456393
51	29.41161794062396	22.317364724003706	21.46105867014587	26.809958665226464
52	30.035676280534148	21.282218279898157	21.974677537689352	26.707427901878344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	8.0
6	16.0
7	9.5
8	3.0
9	13.5
10	24.0
11	219.0
12	414.0
13	801.5
14	1719.5
15	2250.0
16	2374.5
17	2499.0
18	2593.5
19	2688.0
20	3273.5
21	3859.0
22	5269.5
23	6680.0
24	8473.0
25	10266.0
26	13473.0
27	16680.0
28	19496.5
29	22313.0
30	23297.0
31	24281.0
32	23314.0
33	22347.0
34	23903.5
35	25460.0
36	28122.0
37	30784.0
38	33208.5
39	37321.5
40	39010.0
41	42833.5
42	46657.0
43	56205.5
44	65754.0
45	70683.0
46	75612.0
47	92610.0
48	109608.0
49	125859.5
50	142111.0
51	169404.0
52	196697.0
53	225878.5
54	255060.0
55	296330.0
56	337600.0
57	362238.5
58	386877.0
59	404454.0
60	422031.0
61	433207.5
62	444384.0
63	417528.0
64	357387.0
65	324102.0
66	284858.5
67	245615.0
68	211240.0
69	176865.0
70	165664.0
71	154463.0
72	135697.5
73	116932.0
74	88950.0
75	60968.0
76	45139.5
77	29311.0
78	21693.0
79	14075.0
80	8992.5
81	3910.0
82	2575.5
83	1241.0
84	1279.0
85	1317.0
86	733.5
87	150.0
88	106.5
89	35.0
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	4248481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.769817423227323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.58825586448016	7.384834591361631
2	8.29307307936468	1.6204361972574903
3	3.755434103772563	1.1006971661645781
4	2.267861447681425	0.8862636914009412
5	1.418843047532254	0.6930918763302784
6	0.9538493009075459	0.5591360111483846
7	0.6617316359118807	0.4525498086222828
8	0.48534163884759346	0.37933593595447374
9	0.36966858325342283	0.3250435108940145
>10	2.4585513738014915	4.837181963703715
>50	0.6666860129293055	4.891663569864132
>100	2.8881143810441716	64.14426770716516
>500	0.18190360444468714	11.263690777414451
>1k	0.010443064073593522	1.3101905788881556
>5k	2.4286195519984932E-4	0.15161661383029962
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGGAGATCGGGGAGTGCTGGAGATCGGGGGGCGTGGTGGCACAAGCCTAA	6390	0.15040669829993356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.5787951034734532	0.0	0.0	0.0	0.0
7	0.5787951034734532	0.0	0.0	0.0	0.0
8	0.5787951034734532	0.0	0.0	0.0	0.0
9	0.5787951034734532	0.0	0.0	0.0	0.0
10	0.5787951034734532	0.0	0.0	0.0	0.0
11	0.5825376175626065	0.0	0.0	0.0	0.0
12	0.5926588820804424	0.0	0.0	0.0	0.0
13	0.6087116783622193	0.0	0.0	0.0	0.0
14	0.6418529352020169	0.0	0.0	0.0	0.0
15	0.6753707972331758	0.0	0.0	0.0	0.0
16	0.6903408535897889	0.0	0.0	2.35378244600835E-5	0.0
17	0.7084649784240532	0.0	0.0	2.35378244600835E-5	0.0
18	0.7378166455257773	0.0	0.0	2.35378244600835E-5	0.0
19	0.7621782938419638	0.0	0.0	2.35378244600835E-5	0.0
20	0.7888701867796984	0.0	0.0	2.35378244600835E-5	0.0
21	0.815515004068513	0.0	0.0	2.35378244600835E-5	0.0
22	0.8447254442234766	0.0	0.0	2.35378244600835E-5	0.0
23	0.8831627115667929	0.0	0.0	2.35378244600835E-5	0.0
24	0.9170571787893131	0.0	0.0	2.35378244600835E-5	0.0
25	0.953634958000283	0.0	0.0	2.35378244600835E-5	0.0
26	0.9856699370904566	0.0	0.0	2.35378244600835E-5	0.0
27	1.0141742425116176	0.0	0.0	2.35378244600835E-5	0.0
28	1.0484453149254993	0.0	0.0	2.35378244600835E-5	0.0
29	1.084128656806986	0.0	0.0	2.35378244600835E-5	0.0
30	1.125931833048094	0.0	0.0	2.35378244600835E-5	0.0
31	1.1684176061985447	0.0	0.0	2.35378244600835E-5	0.0
32	1.214175136948947	0.0	0.0	2.35378244600835E-5	0.0
33	1.246492569932642	0.0	0.0	2.35378244600835E-5	0.0
34	1.2894490995722943	0.0	0.0	2.35378244600835E-5	0.0
35	1.3180475562912957	0.0	0.0	2.35378244600835E-5	0.0
36	1.3546253355022655	0.0	0.0	2.35378244600835E-5	0.0
37	1.3887081053204664	0.0	0.0	2.35378244600835E-5	0.0
38	1.420484168341579	0.0	0.0	2.35378244600835E-5	0.0
39	1.4511068779641476	0.0	0.0	2.35378244600835E-5	0.0
40	1.4863900768298128	0.0	0.0	2.35378244600835E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	35	1.0204349E-7	46.000004	16
ACTAGGT	35	1.0204349E-7	46.000004	28
ACGATAA	35	1.0204349E-7	46.000004	18
TAACACG	35	1.0204349E-7	46.000004	33
TAGATAA	35	1.0204349E-7	46.000004	31
GCGTAAC	70	0.0	46.000004	31
AGGTATA	25	3.4187236E-5	46.0	15
AACCGAT	20	6.313387E-4	46.0	28
ATTAGTA	45	3.110472E-10	46.0	33
CGTATAG	25	3.4187236E-5	46.0	46
CGTATAC	30	1.8625979E-6	46.0	29
CCGATAT	20	6.313387E-4	46.0	45
ATAATAC	30	1.8625979E-6	46.0	16
ACTCTAT	40	5.6170393E-9	46.0	46
ACCGATA	20	6.313387E-4	46.0	44
TACGGTA	50	1.6370905E-11	46.0	33
CGACAAT	55	1.8189894E-12	46.0	16
CCTAACG	60	0.0	46.0	32
AATATCG	20	6.313387E-4	46.0	30
CGTTAAT	40	5.6170393E-9	46.0	18
>>END_MODULE