FastQCFastQC Report
Fri 17 Jun 2016
SRR1527941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4211544
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGAGATCGGGGAGTGCTGGAGATCGGGGGGCGTGGTGGCACAAGCCTAA61130.14514866756704903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA206.3133787E-446.015
TTAGGCT453.110472E-1046.024
CGTATAG206.3133787E-446.046
ATAATTG301.8625924E-646.021
ATAATTA206.3133787E-446.039
CTATAGG750.046.025
CGATTTC253.4187186E-546.017
CGTTATA551.8189894E-1246.019
CGCCTAT206.3133787E-446.017
TTGCGAT253.4187186E-546.016
AATTAAG206.3133787E-446.041
CCAACTA206.3133787E-446.021
ATTATAC405.6170393E-946.046
ATACGTC301.8625924E-646.014
TATCGCG206.3133787E-446.021
CGATAAA301.8625924E-646.019
ACTATTA351.0204349E-745.99999624
CCTTACG351.0204349E-745.99999634
TAGCGAT351.0204349E-745.99999641
ACTCTAT351.0204349E-745.99999646
Produced by FastQC (version 0.11.5)