FastQCFastQC Report
Fri 17 Jun 2016
SRR1527940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4215479
Sequences flagged as poor quality0
Sequence length59
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGAGATCGGGGAGTGCTGGAGATCGGGGGGCGTGGTGGCACAAGCCTAAAGCACTT65970.1564946711868331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGG307.014987E-753.050
TAGCGTA203.1392064E-453.038
ACCGATA203.1392064E-453.044
ACATTAC203.1392064E-453.041
ACTTATC150.006697553.036
TTATCGC150.006697553.031
CGTTAAT251.4794117E-553.018
CCGTATA251.4794117E-553.019
CATAATT150.006697553.033
ACGTAAT353.3449396E-853.053
CCAACTA353.3449396E-853.021
TAAATCG600.053.038
TATCGCG150.006697553.021
CAATACG203.1392064E-453.029
GCGTAAC650.053.031
CTAATAG307.014987E-753.049
CGGGATA20450.050.40831410
TAACGAG18050.049.7700841
TCGATAA4750.049.65263418
TTACGAG23350.049.368311
Produced by FastQC (version 0.11.5)