FastQCFastQC Report
Fri 17 Jun 2016
SRR1527939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3271743
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA351.0203439E-746.00000446
CATAACG351.0203439E-746.00000438
TTACGAA351.0203439E-746.00000444
GCGCTAA551.8189894E-1246.00000434
CGATCTA351.0203439E-746.00000436
CTAGACG206.313172E-446.046
TCGTTTA301.8624651E-646.041
CGAAATT253.4185497E-546.027
GACGTAT301.8624651E-646.013
TGACGTA453.110472E-1046.029
TAACGGC253.4185497E-546.042
CGCGAAT453.110472E-1046.035
TTATCCG206.313172E-446.046
CGTTAGT206.313172E-446.036
CGAGGTA206.313172E-446.019
CATACGC206.313172E-446.023
GATTGCG2250.046.013
TCGAGTA253.4185497E-546.042
ACCGTAC206.313172E-446.031
CGGTATT206.313172E-446.028
Produced by FastQC (version 0.11.5)