##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527939_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3271743
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72489862437239	33.0	31.0	34.0	30.0	34.0
2	32.331083156592676	34.0	31.0	34.0	30.0	34.0
3	32.49093923330775	34.0	31.0	34.0	30.0	34.0
4	35.99297255316203	37.0	35.0	37.0	35.0	37.0
5	36.0605139217842	37.0	35.0	37.0	35.0	37.0
6	36.02493624957706	37.0	35.0	37.0	35.0	37.0
7	36.11430665550442	37.0	36.0	37.0	35.0	37.0
8	36.10136034523494	37.0	36.0	37.0	35.0	37.0
9	37.672614260961204	39.0	38.0	39.0	35.0	39.0
10	37.687338216968755	39.0	38.0	39.0	35.0	39.0
11	38.06356397797749	39.0	38.0	39.0	37.0	39.0
12	38.05358153131221	39.0	38.0	39.0	37.0	39.0
13	37.97498367078343	39.0	38.0	39.0	35.0	39.0
14	38.35086649532069	40.0	38.0	40.0	35.0	40.0
15	37.343859221216334	39.0	36.0	40.0	33.0	40.0
16	37.253420882997226	39.0	36.0	40.0	33.0	40.0
17	37.2867835279238	40.0	36.0	40.0	33.0	40.0
18	37.413423976149716	40.0	36.0	40.0	33.0	40.0
19	37.52252728897105	40.0	36.0	40.0	33.0	40.0
20	37.49269334418993	40.0	36.0	40.0	33.0	40.0
21	37.34739739643364	40.0	36.0	40.0	33.0	40.0
22	37.40565961323979	40.0	36.0	40.0	33.0	40.0
23	37.333662210020776	40.0	36.0	40.0	33.0	40.0
24	37.39463827079327	40.0	36.0	40.0	33.0	40.0
25	37.51615667856552	40.0	36.0	40.0	33.0	40.0
26	37.494710006256604	40.0	36.0	40.0	33.0	40.0
27	37.489857852526924	40.0	36.0	40.0	33.0	40.0
28	37.41776447599949	40.0	36.0	40.0	33.0	40.0
29	37.4151255156655	40.0	35.0	40.0	33.0	40.0
30	37.364879515292	40.0	35.0	40.0	33.0	40.0
31	37.30592959165802	40.0	35.0	40.0	33.0	40.0
32	37.262055424279964	40.0	35.0	40.0	33.0	40.0
33	37.201924784434475	40.0	35.0	40.0	33.0	40.0
34	37.117554771264125	40.0	35.0	40.0	33.0	40.0
35	37.02886045756039	40.0	35.0	40.0	33.0	40.0
36	36.90714337892677	39.0	35.0	40.0	32.0	40.0
37	36.80624517268013	39.0	35.0	40.0	32.0	40.0
38	36.63532710240383	39.0	35.0	40.0	32.0	40.0
39	36.49576204487944	39.0	35.0	40.0	31.0	40.0
40	36.32908391643231	39.0	35.0	40.0	31.0	40.0
41	36.15157547521306	38.0	35.0	40.0	31.0	40.0
42	35.95477303687973	38.0	35.0	40.0	31.0	40.0
43	35.74843928756018	38.0	35.0	40.0	30.0	40.0
44	35.56069073885082	38.0	35.0	40.0	30.0	40.0
45	35.35581890142349	37.0	35.0	40.0	29.0	40.0
46	35.16363204567107	37.0	35.0	40.0	28.0	40.0
47	34.97680838623327	37.0	34.0	40.0	28.0	40.0
48	34.787893181096436	36.0	34.0	40.0	27.0	40.0
49	34.59875149117764	36.0	34.0	40.0	27.0	40.0
50	34.386008008575246	36.0	34.0	40.0	26.0	40.0
51	34.17193190296426	35.0	34.0	39.0	26.0	40.0
52	33.519455837454224	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	10.0
15	20.0
16	44.0
17	184.0
18	449.0
19	1007.0
20	1803.0
21	3233.0
22	5297.0
23	8106.0
24	11699.0
25	17170.0
26	23406.0
27	31287.0
28	40701.0
29	52448.0
30	65309.0
31	77495.0
32	93953.0
33	120209.0
34	189923.0
35	343277.0
36	263181.0
37	504110.0
38	948281.0
39	469133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.707236479148886	20.875417170602947	36.16121437411191	14.256131976136269
2	27.553661763775455	21.484939373294296	33.52708327029354	17.434315592636707
3	26.702678052646554	21.915168764783786	32.41727727391791	18.96487590865175
4	19.952881384631986	23.763113423028642	34.326901593432005	21.95710359890737
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	100.0	0.0	0.0
10	100.0	0.0	0.0	0.0
11	99.17050330664725	0.7071460074950875	0.08460322219685348	0.0377474636608071
12	98.79281471680386	0.9136720090789527	0.14133139430572633	0.15218187981146442
13	93.69877157221701	3.1910208106199054	1.5652818696334034	1.5449257475296807
14	41.221911378736046	34.936851702594005	9.681903499144035	14.159333419525922
15	28.91394586922017	31.051797161329603	20.832351440806935	19.201905528643294
16	32.23483629368199	20.524656123662524	24.37330805017387	22.867199532481617
17	22.586951358954536	21.215633379516667	27.414041995352324	28.783373266176472
18	22.55060987369729	25.341263051529417	28.84028482677276	23.26784224800053
19	27.642452356435086	23.384721843983467	26.751948426266974	22.22087737331447
20	26.59157519401738	24.51311120708442	26.674955826298092	22.22035777260011
21	30.47476528565966	23.401776973313613	22.24242552058643	23.8810322204403
22	28.076838553639448	26.068765181128224	23.80648480030369	22.04791146492863
23	24.401977783707338	25.232269160505577	25.579026225470642	24.78672683031644
24	25.198097772349477	29.71623993693881	21.600688073604804	23.484974217106906
25	26.708515919496122	23.594671097332522	25.520678121722884	24.17613486144847
26	26.941877769739246	25.84350298907952	21.59558376070492	25.61903548047631
27	28.28752136093819	27.598011212983415	20.356886222420282	23.757581203658113
28	28.266431684884786	27.664061633202852	20.332189906114266	23.7373167757981
29	29.291237117340817	26.01536856654083	19.27941772932654	25.413976586791808
30	29.685155588320967	26.115406986428948	19.703075700016782	24.49636172523331
31	26.381350857937193	26.824386878798244	19.558840654660223	27.235421608604344
32	26.2885257185543	29.796472400185465	19.621620646853984	24.293381234406247
33	28.18207298067116	29.901615132973465	19.67040198450795	22.245909901847423
34	26.539492863589835	30.099063404429995	20.286648431738065	23.074795300242105
35	27.47321534729348	29.964700772646264	19.353842890471533	23.20824098958873
36	26.816806821318178	29.618524437891363	22.022481594672932	21.542187146117527
37	26.320588139105055	30.104717882792137	21.31784189650593	22.256852081596872
38	26.001461606244746	30.734626772335112	22.33323338660769	20.930678234812454
39	26.13533520206202	31.904370239349483	21.099548466979222	20.860746091609272
40	25.58119632257179	32.81999839229426	19.851009079869662	21.74779620526429
41	26.533349349261236	32.25216650574327	19.97714979446735	21.237334350528144
42	25.890603265598795	31.923045300318513	20.76379471125941	21.422556722823277
43	26.699132541889753	31.998754180875455	18.820121262580834	22.48199201465396
44	25.31613882875275	31.13126550587867	19.57983863646992	23.972757028898663
45	25.671117810903855	32.47000146405142	19.626021970552088	22.232858754492636
46	27.505430591583753	29.204800010269754	19.364051516271296	23.925717881875197
47	27.675156636691817	28.53170313193915	20.9538768784712	22.839263352897827
48	28.010818698167917	29.007046091334193	19.999309236697382	22.98282597380051
49	26.949060485496567	29.73301998353783	20.354196524604777	22.96372300636083
50	26.005068246497352	30.810519041379475	20.030974315525395	23.153438396597778
51	24.911553260754282	31.278893238252515	21.093343823154814	22.716209677838386
52	25.46477519780741	30.23327321247421	21.31441864474074	22.987532944977644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	23.5
6	46.0
7	175.0
8	304.0
9	907.0
10	1510.0
11	2553.0
12	3596.0
13	4353.5
14	5056.0
15	5001.0
16	5048.5
17	5096.0
18	6096.0
19	7096.0
20	8854.0
21	10612.0
22	14693.0
23	18774.0
24	25805.5
25	32837.0
26	42099.5
27	51362.0
28	57866.0
29	64370.0
30	66862.0
31	69354.0
32	69187.0
33	69020.0
34	70977.5
35	72935.0
36	76089.5
37	79244.0
38	82935.0
39	90670.5
40	94715.0
41	106721.5
42	118728.0
43	129003.5
44	139279.0
45	150473.0
46	161667.0
47	175463.5
48	189260.0
49	200466.0
50	211672.0
51	215453.5
52	219235.0
53	229870.0
54	240505.0
55	237354.0
56	234203.0
57	242533.5
58	250864.0
59	235656.0
60	220448.0
61	204117.5
62	187787.0
63	160582.5
64	113712.5
65	94047.0
66	80696.0
67	67345.0
68	59085.0
69	50825.0
70	41388.5
71	31952.0
72	28816.5
73	25681.0
74	17212.5
75	8744.0
76	7037.5
77	5331.0
78	3442.0
79	1553.0
80	986.5
81	420.0
82	647.0
83	874.0
84	590.0
85	306.0
86	157.5
87	9.0
88	13.5
89	10.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3271743.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.26559823503725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70756285530419	9.910405139572577
2	7.708713486863358	2.0452139205148483
3	3.5857324291426176	1.4270065735005044
4	2.323902098799509	1.233118063209365
5	1.6420957924209056	1.089169152285043
6	1.158401868094836	0.9220136266117624
7	0.9048648822276918	0.8402501789238801
8	0.7088384465657538	0.7522532836551369
9	0.5781801381880054	0.6902914878612358
>10	3.909194108650956	9.9190677641912
>50	0.7710392430636228	7.5885754428408445
>100	1.8924050055742856	53.67111501384758
>500	0.1004466176067341	8.47385926308865
>1k	0.008623027497561858	1.4376610898973643
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.050279010301236986	0.0	0.0	0.0	0.0
10	0.050279010301236986	0.0	0.0	0.0	0.0
11	0.06171022601714132	0.0	0.0	0.0	0.0
12	0.07870422585148039	0.0	0.0	0.0	0.0
13	0.08793477971833362	0.0	0.0	0.0	0.0
14	0.15676659199698753	0.0	0.0	0.0	0.0
15	0.20946021738260004	0.0	0.0	0.0	0.0
16	0.2408807782273852	0.0	0.0	0.0	0.0
17	0.2647212815921055	0.0	0.0	0.0	0.0
18	0.31338036025445765	0.0	0.0	0.0	0.0
19	0.3435783311831033	0.0	0.0	0.0	0.0
20	0.37781084883500937	0.0	0.0	0.0	0.0
21	0.4124407082096607	0.0	0.0	0.0	0.0
22	0.44982139489562595	0.0	0.0	0.0	0.0
23	0.4875077290606261	0.0	0.0	0.0	0.0
24	0.533140897680533	0.0	0.0	0.0	0.0
25	0.5766039691992922	0.0	0.0	0.0	0.0
26	0.627433144962792	0.0	0.0	0.0	0.0
27	0.6788736156843616	0.0	0.0	0.0	0.0
28	0.7317200648094915	0.0	0.0	0.0	0.0
29	0.7834661830100959	0.0	0.0	0.0	0.0
30	0.8414169450351082	0.0	0.0	0.0	0.0
31	0.9026381350857937	0.0	0.0	0.0	0.0
32	0.9659988574897234	0.0	0.0	0.0	0.0
33	1.0222379936321404	0.0	0.0	0.0	0.0
34	1.077376798850032	0.0	0.0	0.0	0.0
35	1.1248132875962447	0.0	0.0	0.0	0.0
36	1.1786989381500932	0.0	0.0	0.0	0.0
37	1.2355188045026764	0.0	0.0	0.0	0.0
38	1.2960675700994853	0.0	0.0	0.0	0.0
39	1.366335925529603	0.0	0.0	0.0	0.0
40	1.4492275218438613	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	35	1.0203439E-7	46.000004	46
CATAACG	35	1.0203439E-7	46.000004	38
TTACGAA	35	1.0203439E-7	46.000004	44
GCGCTAA	55	1.8189894E-12	46.000004	34
CGATCTA	35	1.0203439E-7	46.000004	36
CTAGACG	20	6.313172E-4	46.0	46
TCGTTTA	30	1.8624651E-6	46.0	41
CGAAATT	25	3.4185497E-5	46.0	27
GACGTAT	30	1.8624651E-6	46.0	13
TGACGTA	45	3.110472E-10	46.0	29
TAACGGC	25	3.4185497E-5	46.0	42
CGCGAAT	45	3.110472E-10	46.0	35
TTATCCG	20	6.313172E-4	46.0	46
CGTTAGT	20	6.313172E-4	46.0	36
CGAGGTA	20	6.313172E-4	46.0	19
CATACGC	20	6.313172E-4	46.0	23
GATTGCG	225	0.0	46.0	13
TCGAGTA	25	3.4185497E-5	46.0	42
ACCGTAC	20	6.313172E-4	46.0	31
CGGTATT	20	6.313172E-4	46.0	28
>>END_MODULE